Figure 5: hiPSC-derived NR progenitors within the 3D RCs recapitulated the spatiotemporal pattern of NR differentiation in vivo. | Nature Communications

Figure 5: hiPSC-derived NR progenitors within the 3D RCs recapitulated the spatiotemporal pattern of NR differentiation in vivo.

From: Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs

Figure 5

(al) Cells within the RCs differentiated and migrated to their corresponding layers, with ganglion cells (GCs; BRN3-positive, (ag); TUJ1-positive, h where * indicates a developing nerve-fibre-like layer) appearing first, followed by photoreceptor cells (PRC; OTX2/recoverin-positive, ac, eg), amacrine cells (AC; AP2α-positive, il) and horizontal cells (HC; PROX1-positive, il). (mp) By W21–W23, RCs presented a well-established outer nuclear layer (REC-positive, m,n) delineated by a developing outer plexiform layer (SV2-positive, n) and containing rod opsin-positive photoreceptors (o) and a developing bipolar cell layer containing bipolar cells (BC; VSX2-positive/MCM2-negative, p) intermingled with the remaining retinal progenitor cells (RPC; VSX2-positive/MCM2-positive, p). (q) Timeline of retinogenesis in hiPSC-derived 3D RCs. Scale bars, 100 μm (ad); 20 μm (ep).

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