Images show a series of experiments, wherein in utero electroporation was done at E12.5 and the brains harvested and analysed at E18.5. In utero electroporation of DCC shRNA and Venus-GFP do not seem to have a major effect on the formation of the CC in wild-type embryos. In utero electroporation of two different DCC shRNAs (shRNA2 and shRNA4) along with Venus-GFP shows a reproducible partial rescue of the CC in Satb2−/− embryos. In utero electroporation of a scrambled shRNA and Venus-GFP cannot rescue the CC in Satb2−/− embryos. Arrows point towards the dorsal midline. Scale bar, 450 μm for low-magnification images and 100 μm for high-magnification images.