(a) RNA-seq data (UCSC browser) and RT–PCR assays (n=2 biological replicates) for AS events developmentally regulated (+ex: exon included; −ex: exon skipped). See Supplementary Fig. 3 and Supplementary Table 3. (b) Correlation between RNA-seq and RT–PCR ΔPSI values. P, Student T distribution. (c) Genes undergoing postnatal AS transitions (|ΔPSI|≥20%) were intersected with differentially expressed (DE) genes in ventricles (E17-adult). (d) AS transitions (|ΔPSI|≥20%; E17-adult) were plotted comparing their change between E17–PN28 (red) and PN28-adult (green). Diagonal line: no difference between time points. Dots above/below the diagonal: increased/decreased inclusion. AS, alternative splicing; bp, base pairs; PSI, percent spliced in.