Figure 3: Consequences of corticosteroid induced obesity in TrkbCck-KO. | Nature Communications

Figure 3: Consequences of corticosteroid induced obesity in TrkbCck-KO.

From: Ablation of TrkB signalling in CCK neurons results in hypercortisolism and obesity

Figure 3

(a,b) Serum leptin levels in young-lean (a) and older-obese (b) TrkbCck-KO and controls measured by EIA. TrkbCck-KO (ratio of control, 2.5 M, TrkbCck-KO, 2.85±1.59 (n=3) versus control 1.00±0.85 (n=3); mean±s.d.; 4.5 M, TrkbCck-KO, 4.65±0.95 (n=6) versus control 1.00±0.13 (n=8), P=0.0008, unpaired Student’s t-test, mean±s.e.m.). (c,d) Serum insulin levels in young-lean (c) and old-obese (d) TrkbCck-KO and controls measured by EIA. TrkbCck-KO (ratio of control, 2.5 M, TrkbCck-KO, 1.10±0.24 (n=3) versus control 1.00±0.46 (n=3), mean±s.d.); 4.5 M, TrkbCck-KO, 1.87±0.30 (n=4) versus control 1.00±0.19 (n=7), P=0.027, unpaired Student’s t-test, mean±s.e.m.). Hormone measurements were replicated 2–3 times. (e,f) Glucose tolerance test in young (e) and old obese (f) TrkbCck-KO and control mice on SC. Glucose (mg dl−1) at 3 M (n=3/4) (0 min, TrkbCck-KO versus control P=0.9; 15 min, TrkbCck-KO versus control P=0.4; 30 min, TrkbCck-KO versus control P=0.2; 60 min, TrkbCck-KO versus control P=0.4; 120 min, TrkbCck-KO versus control P=0.1); at 9 M (n=5/6) (0 min, TrkbCck-KO versus control P=0.3; 15 min, TrkbCck-KO versus control P=0.04; 30 min, TrkbCck-KO versus control P=0.03; 60 min, TrkbCck-KO versus control P=0.1; 120 min, TrkbCck-KO versus control P=0.8, P values by unpaired Student’s t-test. All values are mean±s.e.m.). (g) Liver sections of 11-month-old mice stained with haematoxylin/eosin (upper panels), and Oil-Red-O (lower panels). TrkbCck-KO mice show increased lipid accumulation in TrkbCck-KO mice compared with controls. (h,i) The increase in liver fat content in old TrkbCck-KO mutants is apparent even in freshly dissected liver (liver weight expressed as % of body weight (BW), TrkbCck-KO, 6.7±0.5% (n=3) versus control 4.9±0.2 (n=3), P=0.005, unpaired Student’s t-test, values are mean±s.d.). Livers were analysed in two independent mouse cohorts. Scale bars: (g) upper panel, 50 μm; lower panel, 100 μm; (h) 1 cm.

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