Figure 7: Tailless affects aggression through a neuropeptide-based mechanism. | Nature Communications

Figure 7: Tailless affects aggression through a neuropeptide-based mechanism.

From: Tailless and Atrophin control Drosophila aggression by regulating neuropeptide signalling in the pars intercerebralis

Figure 7

(a) Fighting frequencies of flies expressing UAS-NaChBac in the pars intercerebralis with 50Y-GAL4 and control lines containing only one component of the GAL4/UAS system (Kruskal–Wallis ANOVA, *P=0.0059, n=minimum of 90 pairs for each genotype). (b) Fighting frequencies of flies expressing UAS-tllRNAi and/or UAS-amonRNAi in the pars intercerebralis with 50Y-GAL4 and control lines containing only one component of the binary expression system. Knockdown of tll in the PI induces aggression, which is fully suppressed when amon is simultaneously knocked down (black bar, Kruskal–Wallis ANOVA, ***P<0.001, n=minimum of 60 pairs for each genotype). (c) Fighting frequencies of flies expressing UAS-tllRNAi in the pars intercerebralis with 50Y-GAL4 crossed into a homozygous transposon insertion into Caps, CapsMB03912. Knockdown of tll in the PI induces aggression, which is fully suppressed by a homozygous mutation in Caps (black bar, Kruskal–Wallis ANOVA, ***P<0.001, n=minimum of 60 pairs for each genotype). (d) Co-expression of UAS-nRFP and the heterologous neuropeptide reporter UAS-ANF-GFP (the prepro form of atrial natriuretic factor fused to emerald GFP) in PI neurons with 50Y-GAL4 shows strong expression in the PI and its projections. (e) Co-expression of UAS-tllRNAi and the heterologous neuropeptide reporter UAS-ANF-GFP in PI neurons with 50Y-GAL4 strongly decreases staining in the cell bodies of the PI but not its projections, suggesting increased release. (f) Schematic diagram depicting the regulation of aggression mediated by a Tailless/Atrophin complex acting in the pars intercerebralis on neuropeptide release. Scale bar=100 μm.

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