(a–f) Hippocampal slices were prepared from adult rats and were treated with TEA (20 mM) for 10 min, and collected at the indicated times. TEA and mCalp-I (200 nM) were applied as shown in a and d. (b and e) Representative western blottings for SCOP, PTEN and Akt at the indicated times. (c,f) Quantitative analysis of the levels of SCOP and PTEN (normalized to the values of Akt). In all cases, results are means±s.e.m. of three experiments. *P<0.05, as compared with time point 0, one-way analysis of variance followed by Bonferroni test. (g,h) Effects of mCalp-I on TBS-induced LTP. mCalp-I (200 nM) was applied either before TBS (vertical arrow; blue circles) or 20 min after TBS (red circles). mCalp-I alone had no effect on baseline responses (blue triangles). (h) Summary of the amplitude of LTP measured within the grey area corresponding to 49–50 min after TBS. *P<0.01, as compared with TBS alone.