Figure 6: ERK inhibition prevents and mTOR inhibition mimics calpain inhibition-induced LTP enhancement. | Nature Communications

Figure 6: ERK inhibition prevents and mTOR inhibition mimics calpain inhibition-induced LTP enhancement.

From: A molecular brake controls the magnitude of long-term potentiation

Figure 6

Hippocampal slices were prepared from adult rats and LTP was induced by TBS as in Fig. 1. The MEK inhibitors PD98059 (5 μM) (a) or U0126 (5 μM) (b) were applied together with CI-III (10 μM) for 30 min. The data for the grey symbols in a and b, which were used as the reference, are from Fig. 5a (blue symbol). In all cases, the slopes of the field excitatory postsynaptic potentials (fEPSPs) are expressed as per cent of the average values recorded during the 10-min baseline, and are means±s.e.m. of five to ten slices prepared from three to five animals. (c) Effects of rapamycin (1 μM) applied for 30 min (horizontal bar) starting at 10 min after TBS on hippocampal LTP. (d) Summary of the amplitude of LTP measured within the grey area corresponding to 49–50 min after TBS (note that the data for TBS and TBS+CI-III are from Fig. 5a). *P<0.01, as compared with TBS alone; §P<0.01, as compared with TBS+CI-III (one-way analysis of variance followed by Bonferroni test). The slopes of the fEPSPs are expressed as per cent of the average values recorded during the 10-min baseline, and are means±s.e.m. of five to ten slices prepared from three to five animals.

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