(a,d,g) Experimental protocols: hippocampal slices were prepared from adult rats and were treated with BDNF (100 ng ml−1) for the indicated periods of time in the absence or presence of CI-III (10 μM), as indicated. (b,e,h) Representative western blots for SCOP, Akt, p-ERK and ERK under various experimental conditions. (c,f,i) Quantitative analysis of the levels of SCOP (normalized to the values of Akt) and p-ERK/ERK ratios under various experimental conditions. In all cases, results are means±s.e.m. of three experiments. *P<0.05, as compared with time point 0, one-way analysis of variance followed by Bonferroni test.