(a) Sucrose-banded particles from grapefruit juice (left) and EM visualization of nano-sized particles (middle). Size distribution of the particles was further analysed by Zetasizer Nano ZS (right). (b) Sucrose-banded grapefruit lipid-derived GNVs indicated by the arrow (left) were examined using EM and photographed (middle). Size distribution of the particles was further analysed using a Zeta potential analyser (right). (c) Pie chart with a summary of the putative lipid species in GNVs, reported as percentage of total GNVs lipids. The composition is reported in detail in Supplementary Table S1 in the Supporting Information. (d) GNVs embedded in polyBed 812 were sectioned and examined by electromicroscopy. Original magnification was × 50,000 (left) with enlargement of the indicated area shown in the right panel. (e) Size distribution of the GNVs before (left) and after (right) homogenization was further analysed using a Zeta potential analyser. Data (a,b,d,e) are representative of at least three independent experiments.