Figure 2 : Continuous in vitro culture established by increasing the number of young red blood cells.

From: Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans

Figure 2

(a) PEMR of P. knowlesi H strain in cultures with different proportions of very young red blood cells after one round of reinvasion, as calculated in Figure 1. Statistical significance was determined using Dunnett’s multiple comparison test. Ninety-five per cent confidence interval for five replicates are shown; *P<0.05. (b) Parasitemia of P. knowlesi H strain in cultures with different proportions of very young red blood cells, compared with rhesus blood (small circles), and human blood (large circles). H was continuously cultured in the different proportions by sub-culturing them every 2 days (dashed lines) and parasitemia monitored daily, before and after the split.