Figure 4 : Sema4AF350C protein does not function in a dominant-negative manner.

From: A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration

Figure 4

(a) COS-7 cells were transfected with plasmid constructs encoding Sema4AWT-EGFP, Sema4AD345H-EGFP with or without co-transfection of Sema4AF350C-FLAG. After incubation for 48 h, the cells were stained with an anti-Sema4A antibody and analysed by flow cytometry. Control represents untransfected COS-7 cells with staining in the same conditions. (b) The same transfected COS-7 cells were lysed and subjected to immunoprecipitation (IP) with an anti-Sema4A antibody and subsequent Western blot analyses using anti-GFP (represents Sema4AWT-EGFP or Sema4AD345H-EGFP) or anti-FLAG (represents Sema4AF350C-FLAG) antibodies. Every Sema4A protein was sufficiently expressed in COS-7 cells.