Figure 3 : Rab9 is involved in the trafficking pathway from endosomes to the TGN.

From: Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control

Figure 3

(ac) Confocal images of dextran-injected embryos (a,b) and S2 cells (c) expressing GFP-Rab9. White arrowheads in a and c indicate the endocytic dextran puncta surrounded by GFP-Rab9 at 30 min after dye injection. The boxed region in a was observed by time-lapse imaging, which shows the co-trafficking of the dextran and GFP-Rab9 signals (white arrows in b). (di) Subcellular localization of fluorescent protein-fused Rab9 in the tracheal cells. Stage 16 embryos co-expressing RFP-Rab9 with GFP-Rab5 (d), YFP-Rab7 (e), GalT-CFP (f), GFP-LAMP1 (g) and GFP-KDEL (h) driven by btl-GAL4. (i) Confocal images of the tracheal cells expressing GFP-Rab9 and immunostained with Rab11 antibody. Insets in d, e, f and g show RFP-Rab9 endosomes colocalized with early endosomes, LEs and TGN markers, respectively, and partially colocalized with the lysosome marker (g). No colocalization of fluorescent Rab9 with endoplasmic reticulum (h) or the recycling endosome marker (i) was observed. Scale bar, 2 μm for b and 10 μm for a, ci.