Figure 5 : iPALM measurements of clathrin shape and density.

From: Imaging the post-fusion release and capture of a vesicle membrane protein

Figure 5

(a) Cartoon of a single clathrin triskeleon with a light chain labelled with a fluorescent protein. The triskeleons assemble together to form clathrin-coated structures. (b) Conventional TIRF optical image and (c) iPALM measurement of MLC-mEOS2 in a PC12 cell. Scale bar equals 5 μm. In b, the blue arrows point to gold particles used for alignment. In both cases, a magnified view of the white box is shown below. Scale bar equals 1 μm. (d) Histogram of clathrin spacing in PC12 cells measured with iPALM. (e) Histogram showing the width of the same structures. (f) Plot of width versus depth of the clathrin objects measured with iPALM. Error bars are s.e.m.