Figure 1 : PrPC selectively facilitates zinc uptake.

From: Prion protein facilitates uptake of zinc into neuronal cells

Figure 1

(a) Untransfected SH-SY5Y cells (Un; white bars) or SH-SY5Y cells expressing PrPC (blue bars) were exposed to Zn2+ for 6 h and then stained with 10 μM Zinpyr-1. Kruskal–Wallis, **P<0.01; ***P<0.001. Insert: detection of PrPC in the Un and PrPC-expressing cells with antibody 3F4, with actin as loading control. Molecular weight markers in kDa. (b) Kinetic analysis of the relative increase in zinc uptake using Newport Green in Un SH-SY5Y cells (black symbols) compared with SH-SY5Y cells expressing PrPC (blue symbols), exposed for up to 30 min to 32 μM Zn2+ (n=3). Kruskal–Wallis, P<0.05. The specificity of the Zinpyr-1 staining was determined by exposure of the cells expressing PrPC to (c) alternative divalent cations (100 μM) or the indicated combinations, or (d) specific cation chelators (1,10-phenanthroline (1,10 Phen) 160 μM; N,N,N,N-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) 16 μM; bathocuproine sulphonate (BCS) 16 μM). Data shown as mean (±s.e.m.). Zinpyr-1 fluorescence corrected against DNA content (n=8). Kruskal–Wallis, **P<0.01.