Figure 1 : The general procedure used to merge solution-based small molecule HTS with microarray-based selection of RNAs that bind ligands.

From: Identifying the preferred RNA motifs and chemotypes that interact by probing millions of combinations

Figure 1

(a) Ligands are identified that bind to RNA motif libraries by using a TO-PRO-1 dye-displacement assay. (b) The RNA motifs that bind to each arrayed ligand are identified by microarray-based selection20,21. Briefly, ligands identified that bind RNA from the dye-displacement assay are conjugated to microarray surfaces and hybridized with RNA motif libraries under highly stringent conditions. Bound RNAs are collected from the array by manual excision and sequenced.