Figure 2 : Exploring the salt requirements for self-assembly of a panel of multi-layer DNA objects.

From: Magnesium-free self-assembly of multi-layer DNA objects

Figure 2

(a) Cylinder models of 6, 8, 10, 12, 18, 24, 42 and 100 helix (hlx) bundle objects. Four different versions of the 42-helix bundle object were designed. See Supplementary Fig. S5 for design details and Supplementary Data 1 for DNA sequences for all objects. (b,c) Grey triangles: minimally required magnesium chloride concentrations for obtaining fully folded particles of each of the indicated DNA objects in slow one-pot thermal annealing. Red circles: minimally required sodium chloride concentrations for obtaining fully folded particles in slow one-pot thermal annealing. See Supplementary Fig. S1 for gel electrophoretic data. Blue squares: fraction of DNA staple strands in each object that do not form double-helical DNA domains with the scaffold strand with melting temperatures above 45 °C (methods).