Figure 1 : Exemplary self-assembly of a multi-layer DNA object in the presence of either magnesium or sodium chloride.

From: Magnesium-free self-assembly of multi-layer DNA objects

Figure 1

(a) Schematic illustration of templated molecular self-assembly with DNA origami using one-pot thermal annealing. (b,d) Photographs of ethidium-bromide-stained 2% agarose gels with products obtained from 12-day-long thermal annealing (Supplementary Tables S5) of a multi-layer DNA object in magnesium chloride (MgCl2) (b) and in sodium chloride (NaCl) (d). Saturated high-mobility bands are caused by excess staple strands. The object is designed as 42 DNA double helices packed in parallel on a honeycomb-type lattice. Arrows marked with 'u' indicates unfolded species, whereas 'f' indicates the folded particle species. M, 1 kb marker; ref, scaffold DNA. (c,e) Representative average negative-stain TEM micrographs of folded particles. Particle micrographs were obtained by imaging unpurified folding products directly after thermal annealing in (c) 20 mM magnesium chloride and (e) 2.4 M sodium chloride. Flat view: sum of 300 particles each, side view: sum of 50 particles each. See Supplementary Fig. S2 for individual particles. Scale bar, 20 nm.