Infections by opportunistic fungi have traditionally been viewed as the gross result of a pathogenic automatism, which makes a weakened host more vulnerable to microbial insults. However, fungal sensing of a host's immune environment might render this process more elaborate than previously appreciated. Here we show that interleukin (IL)-17A binds fungal cells, thus tackling both sides of the host–pathogen interaction in experimental settings of host colonization and/or chronic infection. Global transcriptional profiling reveals that IL-17A induces artificial nutrient starvation conditions in Candida albicans, resulting in a downregulation of the target of rapamycin signalling pathway and in an increase in autophagic responses and intracellular cAMP. The augmented adhesion and filamentous growth, also observed with Aspergillus fumigatus, eventually translates into enhanced biofilm formation and resistance to local antifungal defenses. This might exemplify a mechanism whereby fungi have evolved a means of sensing host immunity to ensure their own persistence in an immunologically dynamic environment.
Commensal organisms and mammalian immune mechanisms continuously exchange information, and microbes have developed a contingency-based system during coevolution to guarantee their own persistence in a host by balancing the costs and benefits of expressing virulence. This implies considerable flexibility in virulence manifestation—not to terminate an ongoing symbiosis—and a potential for plasticity in gene expression in response to changes in the host, including the development of acquired immunity. As a consequence, microbes must possess specialized systems that sense and promptly respond to immune activation. Recent progress in our understanding of how fungal signalling circuits operate at the molecular level in sensing environmental factors suggests that this process is much more complex than previously appreciated1,2.
The diploid opportunistic fungal pathogen Candida albicans is a commensal of human mucosal surfaces. Candida infections have typically been thought to rely on an increased susceptibility of weakened hosts to microbial virulence mechanisms. In the case of C. albicans, the increased susceptibility to infection is reflected by fungal transition from a symbiotic to pathogen phenotype. This involves an ability to sense complex environmental signals as well as undergo changes in morphology and virulence3. Genome-wide expression profiling has revealed that the initial contact with the host as well as disease progression is associated with changes in transcriptional expression of C. albicans genes that control metabolic and stress adaptation responses. These adaptive responses occur in a niche-specific fashion2.
Several components of the Candida cell wall have been identified that are capable of binding host proteins and will, at the same time, affect growth, morphogenesis and virulence of the fungus4,5,6. In line with what is observed in humans7, Candida overgrowth occurs under conditions of defective interleukin (IL)-17F8 or IL-17RA signalling8,9,10, which suggests the importance of IL-17RA signalling in preventing infection. Under specific circumstances, IL-17A may, in fact, contribute to disease susceptibility in a host-autonomous fashion, perhaps by modifying the intrinsic virulence of the fungus8. In this study, we provide molecular evidence that fungal pathogens may exploit, via a conserved family of glycosylphosphatidylinositol (GPI)-anchored crosslinking proteins, host immunity mediators—specifically, IL-17A—for adaptation, not necessarily implicating virulence, and pathogenesis in mammalian hosts.
IL-17A promotes fungal aggregation and filamentation
We studied any possible direct effects of IL-17A on C. albicans. We cultured Candida cells in an amino-acid-rich liquid medium (RPMI-1640), which is generally considered to be a physiologically relevant environment11—at 37 °C for 24 h or under conditions of biofilm formation. IL-17A was added at concentrations of 1 or 10 ng ml−1, and cells were examined by light or scanning electron microscopy (SEM) (Supplementary Methods) at different times. IL-17A increased cell aggregation at 4 h (Fig. 1a) and germination at 24 h (Fig. 1b) in a dose-dependent fashion. The percentage of germinated cells was not increased at 4 h, a time when only 10–15% of the treated cells had germinated, as opposed to >40% germinated, control cells (Fig. 1c). IL-17A also enhanced biofilm formation, as proven by profuse growth and dense aggregation on SEM examination (Fig. 1d,e). IL-17A-treated cells were metabolically active, as shown by viable cell counts (Fig. 1f) and staining profiles with a fluorescent probe (FUN-1; Fig. 1g–l) (Supplementary Methods). By epifluorescence microscopy (590-nm excitation; 610-nm emission), C. albicans cells treated with IL-17A and stained with FUN-1 manifested bright red–orange fluorescent structures that correlated with fungal metabolic activity and viability. FUN-1 staining also indicated the presence of true hyphae.
Morphological transition in Candida occurs in response to a variety of environmental factors, including amino-acid availability and pH changes2,12,13. To determine whether the effects of IL-17A on the fungus were unique to specific experimental settings, cells were exposed to IL-17A under conditions of limited (SPIDER medium) or rich (YPD medium) nutrient availability, nitrogen starvation (SLAD medium) or alkaline pH (M199 medium), in liquid or solid cultures. IL-17A increased cellular aggregation in SPIDER, SLAD and M199 but not in YPD media (Supplementary Fig. S1a). IL-17A also promoted filamentation in SPIDER and M199 media under liquid (Supplementary Fig. S1b) or solid (Supplementary Fig. S1c) culture conditions, suggesting that IL-17A may exploit pathways of cellular aggregation and filamentation under permissive conditions. The lack of filamentation observed in SLAD medium might indicate that aggregation and filamentation occur independently of each other.
To determine whether other cytokines could mimic the effects of IL-17A, we exposed C. albicans to murine IL-17F, IL-17A/IL-17F heterodimer, IL-17E, IL-12p70, IL-23, interferon (IFN)-γ, IL-4 or IL-10. Of these cytokines, only IL-17F and the IL-17A/IL-17F replicated the effects of IL-17A (Supplementary Fig. S1d). Consistent with previous data14, germination was inhibited by IFN-γ, an effect opposed by the co-presence of IL-17A, both in vitro (Supplementary Fig. S1d) and in vivo in C. albicans infection (Supplementary Fig. S1e–h). Thus, IL-17A at concentrations encompassing or exceeding those measured in infected organs of wild-type and, particularly, IL-17F-deficient mice (Supplementary Fig. S2a–i) may affect fungal growth and morphology. However, owing to rapid degradation, the concentrations of IL-17A measured in ex vivo samples might be considerably lower than those in actual tissue microenvironments15.
IL-17A affects the transcriptional profile of C. albicans
We focused on potential signalling events triggered by IL-17A in C. albicans. We examined the genome-wide transcriptional profile of the fungus exposed to 10 ng ml−1 IL-17A for 4 or 24 h at 37 °C in RPMI-1640 liquid medium. Gene expression analysis indicated that more than 60 transcripts were affected by short-term (4 h) exposure to IL-17A (Fig. 2a). Of these, 40 genes encoding components of the translational machinery were downregulated transcriptionally (Supplementary Data 1). Downregulated genes related to ribosome biogenesis, permease/transporter functions, filamentous growth, meiosis, rRNA-processing enzymes, transcription, glycolysis and protein synthesis. At the same time, short-term exposure to IL-17A doubled the expression of 21 genes (Fig. 2a). Among those were genes involved in autophagy, a major eukaryotic process by which bulk cytoplasmic components are degraded in the vacuole/lysosome in response to starvation (Fig. 2b and Supplementary Data 1). Those genes included RIC1, which is related to intracellular trafficking16 and proteins of the vacuolar protein sorting (Vps) system, such as the Candida orthologue (orf. 19.130) of the serine/threonine protein kinase Vps15. Vps15 has a role in yeast autophagy in response to nutrient starvation17, and functions as a membrane-associated complex with class III phosphoinositide 3-kinase17,18. Overall, the IL-17A-modulated transcriptional profile mirrored that induced in C. albicans by rapamycin4, suggesting a role for inhibition of the target of rapamycin (TOR) signalling pathway in the activation of autophagy by IL-17A. Consistently, the TOR inhibitor LPF18 and the downstream orf. 19.4325 gene—an orthologue of Saccharomyces cerevisiae MUC1 coding for an adhesion protein19—were upregulated (Fig. 2c).
Although the patterns observed for some genes at 4 h occasionally persisted over time (such as the upregulation of MUC1), the transcriptional profile observed after prolonged exposure to IL-17A was considerably different. Candida exposure to the cytokine for 24 h resulted in the induction of 58 genes (Fig. 2d) involved in cell mitosis, ribosome biogenesis, transcription and cell wall remodelling (Fig. 2e and Supplementary Data 1). Of interest, classic hyphae-induced genes were increased in expression, such as BNI4 (orf 19.4457)20 and an orthologue of S. cerevisiae adenylate cyclase Cyr1 (orf. 19.5258) (Fig. 2f). Cyr1p is a key component of the cAMP/PKA signaling pathway directly involved in signal sensing and in controlling hyphal morphogenesis in C. albicans21.
To substantiate the global transcriptional profiling data, we performed western blotting, fluorescence microscopy, real-time quantitative reverse transcriptase PCR and enzyme-linked immunosorbent assays to measure the levels of proteins or monitor functions associated with the differentially regulated genes (that is, TOR1, autophagy and extracellular/intracellular cAMP). Similar to the effect of rapamycin, TOR1 expression was reduced after 4 h (Fig. 2g) but not 24-h (Fig. 2h) exposure to IL-17A and, accordingly, autophagy was induced, as revealed by ATG8 immunofluorescence (Fig. 2i–n), monodansilcadaverine (MDC) labelling (Fig. 2o–r) and increased expression of the autophagy-related genes (Fig. 2s). We used the C. albicans TOR1-1/TOR1 mutant4 to evaluate the role of the TOR pathway in IL-17A signalling. We found that the effects of IL-17A on autophagy-related gene expression (Fig. 2t) and cell aggregation (Supplementary Fig. S3a–d) could not be observed in this mutant. At variance with rapamycin, intracellular cAMP levels (Fig. 2u,v) and expression of the Cyr1 orthologue gene (Fig. 2w) were increased after 24-h exposure to IL-17A. Thus, although autophagic flux was not measured, the data suggest that the TOR pathway mediates autophagy and cell aggregation in response to IL-17A but is dispensable for hyphal induction. In fact, filamentation of the TOR1-1/TOR1 mutant was observed after exposure to IL-17A (Supplementary Fig. S3c,d).
IL-17A binds C. albicans
C. albicans binds a variety of host proteins that affect fungal morphology and virulence4,6. We examined whether IL-17A directly binds the fungus in vitro. An indirect immunofluorescence assay revealed the presence of Candida cells with bound IL-17A. Binding was affected significantly by growth phase of the fungus, as intense binding was observed on hyphal tips and not on parent blastospores (Fig. 3a–g). Not all hyphal tips stained with the same intensity. Thus, IL-17A binds C. albicans components expressed during hyphal growth. To assess whether ex vivo Candida cells also bind IL-17A, we used C. albicans isolates freshly obtained from different human anatomical sites. All of the analysed C. albicans isolates, and particularly those from the gut and vagina, were able to bind murine (Fig. 3h) and human (Fig. 3i) IL-17A. Notably, freshly isolated samples from vaginal fluids stained positive with an anti-IL-17A antibody (Fig. 3j–o), suggesting that binding to IL-17A may occur in vivo at the infected sites. Levels of IL-17A in human vaginal fluids ranged from 80 to 240 pg ml−1. Murine studies confirmed that IL-17A may bind to the fungus in the vagina as revealed by the presence of numerous hyphae in the vagina of IL-17F-deficient (that is, in conditions of high levels of IL-17A) but not IL-17A-deficient or wild-type mice intravaginally injected with C. albicans blastospores (Fig. 3p–r). Binding was also observed, albeit to a lesser extent, with other yeast species, such as C. glabrata, C. krusei and S. cerevisiae (Fig. 3s).
IL-17A binds GPI-anchored fungal proteins
To identify the putative fungal ligand(s) involved in IL-17A effects, we studied binding of IL-17A to purified proteins and polysaccharides from the fungal cell wall. IL-17A selectively bound members of the Crh and Gas GPI-anchored protein family, namely Crh11p and Gas1p, and did not bind fungal polysaccharides (Fig. 4a). No binding was observed with IL-17E (Fig. 4a) or other cytokines (data not shown). Anti-IL-17A or anti-Crh11p antibody inhibited binding (Supplementary Fig. S4a), and Crh11p inhibited binding of IL-17A to whole fixed hyphae (Supplementary Fig. S4b). A strong protein-to-protein interaction was observed in co-immunoprecipitation experiments with anti-IL-17A and anti-Crh11p (Fig. 4b). These data suggested that fungal Crh11p might act as a binding structure (that is, an acceptor rather than a signalling receptor) for host-derived IL-17A. Notably, IL-17A failed to bind mutant Candida strains in which all three genes of the CRH family (namely, UTR2, CRH11 and CRH12) had been deleted22, yet binding was restored by CRH11 or CRH12 reconstitution (Fig. 4c). Consistent with the notion that Crh11p, more than Crh12p, is cell cycle regulated and expressed under sporulation conditions23, of the two mutant strains, crh11Δ but not crh12Δ failed to respond to IL-17A with adhesion and filamentation (Supplementary Fig. S4c,d) (Supplementary Methods) as well as with internalization of bound IL-17A (Fig. 4d,e). Thus, IL-17A binds different members of the CRH family in Candida, and, specifically, Crh11p appears to be necessary for IL-17A effects on the fungus.
IL-17A sensing in vivo
As both adhesion and hyphal growth are considered to be important for pathogenicity24, we assessed whether the increased fungal adhesion and filamentation induced by IL-17A might have biological relevance in vivo. For this purpose, we evaluated whether binding to IL-17A could be demonstrated on fungi in vivo, the consequences in terms of fungal growth and morphology, and the involvement of the CRH family proteins. We found that IL-17A bound the fungus in the stomachs of wild-type (Fig. 5a–c), IL-17F-deficient (Fig. 5d–f) but not IL-17A-deficient (Fig. 5g–i) mice intragastrically infected with the fungus, and this was associated with germination in wild-type mice (Fig. 5j), hyphal formation in IL-17F-deficient mice (Fig. 5k) and neither one in IL-17A-deficient mice (Fig. 5l). Thus, despite the ability of IL-17F to mimic IL-17A effects in vitro (Supplementary Fig. S1d), a lower production of IL-17F in vivo (Supplementary Fig. S2g–i) might account for the lack of IL-17F activity on the fungus at sites of infection. We also evaluated the effects of pre-exposure of C. albicans to IL-17A in vitro on fungal growth and virulence in the orogastric as well as the oropharyngeal models of candidiasis. We found that IL-17A-treated Candida cells were more virulent in vivo, as indicated by increased fungal load (Fig. 5m) and local inflammatory pathology in the stomachs of intragastrically infected mice (Supplementary Fig. S5a,b). Consistent with the in vitro data, the crh11Δ, but not the crh12Δ, mutant was less virulent in vivo and failed to respond to IL-17A with increased virulence (Fig. 5n,o and Supplementary Fig. S5c–f). Similar results were obtained in the oropharyngeal model of candidiasis (Supplementary Fig. S5g–r). Thus, fungal sensing of IL-17A occurs in vivo via Crh1p, and affects fungal growth and morphology.
IL-17A induces fungal autophagy in vivo
In searching for an explanation for the increased fungal outgrowth, we determined whether IL-17A would favour fungal evasion by assessing susceptibility to killing by effector phagocytes in vitro. We observed that IL-17A increased fungal adhesion on phagocytes, yet it reduced phagocytosis and susceptibility to killing of C. albicans SC5314 (Fig. 6a). Although C. albicans may not depend on autophagy for virulence25,26, we found that adhesion to phagocytes and susceptibility to killing of the mutant strains TOR1-1/TOR1 (Fig. 6b) and the atg9Δ/atg9Δ, blocked in autophagy5,26 (Fig. 6c), were not similarly affected by IL-17A. Thus, much like in Cryptococcus neoformans18, the TOR-dependent autophagy pathway might be involved in virulence in vivo. To clarify this issue, we evaluated whether autophagy could be demonstrated at sites of infection, and the impact on fungal adaptation and virulence. Autophagy (Fig. 6d–f) and upregulated expression of autophagy-related genes (Fig. 6g) were observed in ex vivo Candida SC5314 cells from IL-17F-deficient but not IL-17A-deficient or wild-type mice. Similar to that observed with the crh11Δ mutant, a reduced fungal growth and inflammatory pathology were observed with the TOR1-1/TOR1 or the atg9Δ/atg9Δ mutant strains but not the wild-type or the atg9Δ/atg9Δ+ATG9 revertant strains under conditions of IL-17A production, such as in wild-type (Fig. 6h and Supplementary Fig. S6a–d) and IL-17F-deficient mice (Fig. 6i and Supplementary Fig. S6e–g), but not in the condition of IL-17A deficiency (Fig. 6j and Supplementary Fig. S6h–j). Thus, IL-17A sensing via fungal Crh proteins affects fungal fitness in vivo through adaptation mechanisms involving TOR-dependent autophagy and filamentation.
A. fumigatus senses IL-17A
To assess whether A. fumigatus—a ubiquitous fungus that, although not a commensal in the strict sense, is nevertheless in continuous contact with the human upper respiratory tract27—also senses IL-17A, we performed binding studies and morphological assays in vitro and in vivo. IL-17A strongly bound to the fungus. Binding was dependent on growth phase of the fungus, being undetectable in resting conidia, but intense in swollen conidia (Fig. 7a–f). The fungus responded to IL-17A with biofilm formation (Fig. 7g,h) and, similar to rapamycin (Fig. 7i) with autophagy (Fig. 7j,k). As levels of lung IL-17A ranged from 1.5 (wild-type) up to 9 ng ml−1 (IL-17F-deficient mice) during infection, these data suggest that IL-17A can affect Aspergillus morphology in vivo.
Orthologues of the GPI CRH family are present in Aspergillus28. We assessed whether IL-17A would bind those proteins. Of the two orthologous proteins, Crf1p but not Gel1p bound IL-17A (Fig. 7l). As in the Candida setting, no binding was observed to Aspergillus-derived polysaccharides (Fig. 7l). IL-17A failed to bind an Aspergillus mutant (Fig. 7m) in which the CRF1 gene had been deleted (Supplementary Fig. S7a,b). The mutant strain failed to respond to IL-17A in vitro with extensive hyphal growth after 24 h of exposure (Fig. 7n,o), as opposed to the wild-type strain (Fig. 7p,q). In vivo, the virulence of the mutant strain was not affected upon IL-17A pre-exposure in vitro, as indicated by fungal growth (Fig. 7r) and histopathology (Supplementary Fig. S7c,d) as opposed to the wild-type strain (Fig. 7s and Supplementary Fig. S7e,f). Thus, IL-17A sensing is a feature shared by human commensal and airborne fungi.
This study provides molecular evidence that opportunistic pathogens, such as C. albicans and A. fumigatus, may exploit host immunity mediators—specifically, a cytokine—for adaptation to their hosts. Thus, in common with certain opportunistic bacteria—such as Pseudomonas aeruginosa29—human fungi might have evolved a contingency-based mechanism to mount an effective countermeasure to immune activation by their host. Although it was previously recognized that IFN-γ inhibits yeast-to-hyphal transition in C. albicans14, little progress had been made on the mechanistic details of this early observation. Our data show that a conserved family of GPI-anchored crosslinking enzymes, highly conserved in Candida and Aspergillus, and known to be exposed at growing sites—such as bud emergences or hyphal tips22,23—and detectable in mice28,30,31 as well as in humans32, mediates IL-17A sensing by fungi. Thus, in addition to their involvement in adhesion and virulence6,12, these proteins are immunologically reactive. Intriguingly, our study also suggests that Th1 and Th17 cytokines may have disparate effects on Candida in vitro (Supplementary Fig. S1d), as well as in vivo (Supplementary Fig. S1e–h), a finding highlighting the complex cross-regulation between Th1 and Th17 responses that would also include an action on fungal cells.
Host IL-17A sensing occurs via the TOR nutrient sensing pathway, a conserved mechanism among yeasts and filamentous fungi33 that regulates autophagy34, cell growth and virulence4,18,35. Autophagy is an evolutionary conserved response to stress that has been linked to important protective roles in mammalian cells36. In mammalian host–pathogen interactions, the role of autophagy has mostly favoured the host, as induction of autophagy was found to be crucial in the control of some infecting pathogens and in the ensuing immune response37,38. This study highlights how the TOR nutrient sensing pathway could be exploited by fungi to sense host IL-17A for survival in an immunologically reactive environment, consistent with the view that Th17 immunity develops to maintain mutualism39. However, prolonged exposure to IL-17A further affects fungal fitness by inducing filamentation and virulence in a TOR-autonomous fashion. Thus, IL-17A may contribute both to fungal survival, not necessarily implicating virulence, and to pathogenesis in mammalian hosts. Despite a plethora of mechanisms by which autophagy and virulence can be regulated in different fungal infections, our study highlights the unique feature of the amino-acid sensing pathway, a survival strategy under evolutionary pressure40, in the interaction of fungi with their mammalian hosts.
Strains and growth media
A complete list of the strains used in this study is reported in Supplementary Table S1. C. albicans strains were propagated on Sabouraud's glucose agar 37 °C (Sigma Aldrich, Milan, Italy). Isolation of C. albicans from infected stomachs was done on a sucrose gradient, as described41. Viable Aspergillus conidia (>95%) were obtained by growth on potato dextrose agar (PDA) with chloramphenicol 0.05% [w/v], (Difco Laboratories, Detroit, MI, USA) after 4 days at room temperature (RT). Freshly harvested (unpassaged) C. glabrata, C. krusei and S. cerevisiae isolates, obtained from clinical specimens, and C. albicans clinical isolates, derived from blood, urine, vagina and gastrointestinal tract, were kindly provided by the Mycology Section, S. Maria della Misericordia Medical Center, Perugia, Italy. Study approval was provided by the local ethics committee (Umbria Regional Hospital Ethics Committee, CEAS Umbria), and informed written consent was obtained from all participants in accordance with the Declaration of Helsinki. The CAG31A C. albicans mutant strain expressing the GFP gene was obtained as described42. When observed by fluorescence microscopy, both yeasts and hyphae of the CAG31A strain were intensively fluorescent43.
IL-17A binding to fungi by indirect immunofluorescence
Localization of IL-17A binding on C. albicans and A. fumigatus was assayed by indirect immunofluorescence using a PE-rat IgG2a raised against mouse IL-17A (clone eBio17B7, e-Bioscience, Prodotti Gianni Milan, Italy). Fungal cells were collected, washed, resuspended in RPMI-1640 medium, placed (1×106 cells ml−1) on microscope glass slides and incubated overnight at 28 °C for Aspergillus (AF293) and for 4 h at 37 °C for Candida GFP (CAG31A). After adhesion, cell cultures were fixed with 1% formaldehyde. Slides were washed with PBS. Nonspecific binding sites were blocked with PBS/bovine serum albumin (BSA) 2.5% and incubated for 1 h at RT. Cells were incubated with IL-17A (10 ng ml−1) for 4 h at RT and then incubated with anti-IL-17A-PE (0.5 μg ml−1) in PBS/BSA 2.5% for 1 h at RT. Slides were washed with PBS and mounted on a cover slip with a few drops of Vectashield mounting medium (Vector Laboratories, DBA Italia S.R.L. Milan, Italy). For staining of fungi isolated from the stomachs of Candida GFP-infected mice, tissues were harvested 2 days post infection and processed in RPMI-1640. For staining of Candida from human vaginal fluids, cells recovered after clarifications were stained with Con A-FITC conjugate (25 μg ml−1). After blocking of nonspecific binding sites with PBS/BSA 2.5%, the cytospins were incubated for 1 h at RT with PE-anti-human IL-17A (0.5 μg ml−1, clone 41802, R&D System). Stained cells were observed by fluorescence microscopy at ×40 magnifications using the Olympus BX51 upright microscope.
For microarray analysis, cultures of C. albicans SC5314 cells were grown in RPMI-1640 medium and treated with either 10 ng ml−1 IL-17A (R&D Systems) or drug vehicle, and incubated for 4 and 24 h at 37 °C. Total RNA was extracted with mortar and pestle in the presence of liquid nitrogen. The resulting powder was resuspended in TRIZOL (Invitrogen S.R.L., Milan, Italy) and transferred to a 1.5-ml Eppendorf tube, and gently vortexed and inverted two or three times to homogenize the sample. RNA was then amplified, biotin-labeled (MessageAmp II Biotin-Enhanced kit, Ambion Applied Biosystems, Monza, Italy) and purified on an affinity resin (RNeasy, Qiagen SpA, Milan, Italy). Hybridization was done automatically overnight (16 h) at 45 °C to Affymetrix yeast GeneChips according to the protocol in the Affymetrix GeneChip Expression Manual using Febit's RT Analyzer. Starting from raw data files, analysis was performed using both the Affymetrix MAS4 algorithm (at the service provider) and the PROP algorithm (at Cebit, Germany). Microarray data were generated from two biological replicates. Complementary DNA generated from cells exposed to drug vehicle alone was used as reference. Significantly modulated genes were selected from those whose expression was above twofold higher or lower than control samples (accession code GSE35153).
Gene Ontology annotations
Protein function was assigned using a standard set of Gene Ontology (GO) Slim biological process term sets available at the Candida Genome Database (http://www.candidagenome.org).
Analysis of autophagy
ATG8 localization by indirect immunofluorescence
Localization of ATG8 in Candida SC5314 cells was assayed by indirect immunofluorescence using a rabbit polyclonal antibody against ATG8 (Abcam, Prodotti Gianni, Milan, Italy). Localization of ATG8 was performed on cells grown in RPMI-1640 supplemented with glutamine and 2% dextrose, and buffered with 0.165 M MOPS, pH 7. Cells (1×106 ml−1) were placed on microscope glass slides, incubated for 4 h at 37 °C for adhesion, and treated with 10 ng IL-17A or drug vehicle for additional 4 h at 37 °C. Nonspecific binding sites were blocked with PBS/BSA 2.5%. Slides were then sequentially incubated with Con A for 45 min at 37 °C, with 1:1,000 dilution of a rabbit polyclonal antibody to ATG8 in PBS/BSA 2.5% at 4 °C and finally with 1:50 dilution of anti-rabbit IgG secondary antibody conjugated to tetramethyl rhodamine isothiocyanate (TRITC) (Sigma Aldrich). Cells were visualized with a 40×0.75 magnification objective on the Olympus BX51 upright microscope.
MDC labelling was performed on Candida SC5314 grown as above and placed on microscope glass slides in the presence of 1 and 10 ng ml−1 IL-17A. A stock solution of 25 mM MDC in ethanol–DMSO (1:1—vol:vol) was prepared. After 4 h, cells were stained with the fluorescent dye MDC (Sigma Aldrich) at a final concentration of 50 μM for 20 min in the dark. 4′-6-Diamino-2-phenylindole (DAPI, Molecular Probes, Invitrogen S.R.L.) was used to detect nuclei. After washing with water, cells were visualized with a 40×0.75 magnification objective on the Olympus BX51 upright microscope.
Mice and infections
Mice were housed in specific pathogen-free barrier facilities at the Animal Facility of Perugia University, Perugia, Italy and were used in accordance with protocols approved by the Animal Welfare Assurance A-3143-01. Female C57BL/6 mice, 8- to 10-week-old, were purchased from Charles River laboratories (Calco). Breeder pairs of IL-17A−/− and IL-17F−/−, generated as described44 were kindly provided by Dr Burkhard Becher, University Hospital of Zurich, CH-8057 Zürich, Switzerland. For Candida infection, 108 cells of C. albicans wild-type SC5314 strain and its mutant strains atg9Δ/atg9Δ, the revertant atg9Δ/atg9Δ+ATG9 and TOR1-1/TOR1, or the MKY 378 strain and the derived mutants, crh11Δ and crh12Δ were injected intragastrically. The infections with the two wild-type strains were similar, and the growth rate of the mutant strains was comparable to that of the corresponding wild-type strains in vitro (data not shown). For vaginal infection, estrogen-treated mice (0.1 mg of estradiol benzoate (Sigma Aldrich) in 0.1 ml oil subcutaneously every other day) were intravaginally inoculated with 5×104 stationary phase wild-type or crh11Δ blastoconidia as described45. Two days post infection, lavage fluid cytospins were observed for fungal growth by light microscope, after May–Grünwald–Giemsa staining. For pulmonary aspergillosis, mice were anesthetized by intraperitoneal injection of 2.5% avertin (Sigma Chemical) before instillation of a suspension of 2×107 conidia per 20 μl saline intranasally of the Aspergillus AF293 wild-type and the crf1Δ mutant strains. Cells were exposed to IL-17A (10 ng ml−1) or drug vehicle for 4 h at 37 °C in RPMI-1640 before injection. Mice were monitored for fungal growth and histology. Fungal growth was expressed as colony-forming units per organ, mean±s.e., obtained by serially diluting homogenates and plating them in triplicate on Sabouraud agar plates. For histology, paraffin-embedded tissue sections (3–4 μm) of stomach and lung tissues were stained with periodic acid-Schiff reagent. Photographs were taken using the Olympus BX51microscope. For SEM, the stomachs were removed at 2 days post infection and processed for SEM staining.
For multiple comparisons, P-values were calculated by using one-way analysis of variance (Bonferroni post-test), as specified for individual P-values described in figure legends. For single comparison, P-values were calculated by using two-tailed Student's t-test. The data reported are either from one representative experiment out of three to five independent experiments or are compiled from three to five experiments. At least six mice per group per experiment were used, as computed by power analysis to yield a power of at least 80% with an α-value of 0.05.
Accession codes: Microarray data have been deposited in Gene Expression Omnibus under the accession code GSE35153.
How to cite this article: Zelante, T. et al. Sensing of mammalian IL-17A regulates fungal adaptation and virulence. Nat. Commun. 3:683 doi: 10.1038/ncomms1685 (2012).
Gene Expression Omnibus
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Robert J. Bastidas provided reagents for this work. We thank Joseph Heitman for critical discussion. This work is supported by the Specific Targeted Research Project ALLFUN (FP7 – Health – 2010–single–stage, contract number 260338) and by the Fondazione per la Ricerca sulla Fibrosi Cistica (Research Project number FFC#21/2010 to L.R. with the contribution of funded Francesca Guadagnin, Coca Cola light Tribute to Fashion and Delegazione FFC di Belluno). We thank Cristina Massi Benedetti from the University of Perugia for editorial assistance.
The authors declare no competing financial interests.
Supplementary Figures S1-S7, Supplementary Tables S1-S2, Supplementary Methods and Supplementary References (PDF 4851 kb)
Genes upregulated/downregulated in Candida albicans SC5314 cells grown in RPMI 1640 medium at 37°C in the presence of IL-17A for 4h or 24h. (XLS 63 kb)
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Zelante, T., Iannitti, R., De Luca, A. et al. Sensing of mammalian IL-17A regulates fungal adaptation and virulence. Nat Commun 3, 683 (2012). https://doi.org/10.1038/ncomms1685
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