Fox odour 2,4,5-trimethyl thiazoline (TMT) is known to activate multiple glomeruli in the mouse olfactory bulb (OB) and elicits strong fear responses. In this study, we screened TMT-reactive odourant receptors and identified Olfr1019 with high ligand reactivity and selectivity, whose glomeruli are located in the posterodorsal OB. In the channelrhodopsin knock-in mice for Olfr1019, TMT-responsive olfactory-cortical regions were activated by photostimulation, leading to the induction of immobility, but not aversive behaviour. Distribution of photoactivation signals was overlapped with that of TMT-induced signals, but restricted to the narrower regions. In the knockout mice, immobility responses were reduced, but not entirely abolished likely due to the compensatory function of other TMT-responsive glomeruli. Our results demonstrate that the activation of a single glomerular species in the posterodorsal OB is sufficient to elicit immobility responses and that TMT-induced fear may be separated into at least two different components of immobility and aversion.
In the mammalian brain, sensory information is spatially encoded and forms neural maps that are fundamental for higher-order processing. In the mouse olfactory system, odour signals detected by olfactory sensory neurons (OSNs) in the olfactory epithelium (OE) are represented as an odour map in the olfactory bulb (OB)1,2,3. Because each glomerulus corresponds to a single odourant receptor (OR)3,4, and a single odourant can interact with multiple OR species5,6, odour signals received in the OE are converted into a topographic map of multiple glomeruli activated in varying magnitudes2,5,6,7,8,9. The main olfactory system in rodents is known to mediate innate aversive behaviours to spoiled foods, and fear responses to predator odours10,11,12,13,14. One predator odourant is 2,4,5-trimethyl thiazoline (TMT), a molecule secreted from the fox anal gland. TMT induces strong fear responses in rodents including not only aversion but also immobility that is not induced by other aversive odours11,12. We previously reported mutant mice, ΔD10, in which glomeruli were ablated by the zone-specific expression of diphtheria toxin in the dorsal OB, and demonstrated their failure to elicit innate fear upon exposure to TMT. Optical imaging of the dorsal OB has revealed that these TMT-responsive glomeruli in the dorsal domain are clustered to a posterior part of the DII subdomain9. Although multiple glomeruli are activated in both dorsal and ventral domains by TMT, it was uncertain whether individual glomeruli are functionally specialized to instruct particular responses, or whether a pattern of activated glomeruli as a whole is recognized to mediate behavioural decisions. It was also unclear whether the TMT-induced fear responses can be further divided into different categories, such as immobility and avoidance. To address these questions, we performed loss-of-function and gain-of-function experiments to determine the effect on mouse behaviour when a single glomerular species, responsive to the fox odourant TMT, is deleted or photoactivated.
Isolation of cDNAs for TMT-responsive ORs
For gene-targeting experiments, we searched for candidate ORs with high affinity and selectivity for TMT. Although TMT activates multiple glomeruli in both the dorsal and ventral OBs10, the glomeruli responsible for inducing innate fear appear to be confined to the DII subdomain of the OB9. We, therefore, analysed the dorsal OB by optical imaging, after exposure to varying concentrations of TMT (Fig. 1a and Supplementary Fig. 1a). A related odourant 4-methyl-thiazoline (4MT) that induces aversion but not immobility14 was examined in parallel. A large area of the dorsal OB was activated in the presence of high concentrations of TMT. However, the number of activated glomeruli narrowed as the TMT concentration was lowered. These glomeruli were located in the posterior part of the dorsal OB (Fig. 1a). To clone the OR genes associated with these glomeruli, we retrogradely labelled the connecting OSNs15,16 by injecting DiI solution into the single glomeruli activated by TMT, but not by 4MT. At 36 h after the DiI injection, we confirmed that the dye had specifically stained OSNs in the dorsal OE (Supplementary Fig. 1b). OE cells were dissociated and DiI-positive OSNs were subjected to single-cell PCR with reverse transcription for OR gene cloning5,15. After the completion of five independent experiments, 12 OR complementary DNAs (cDNAs) frequently and repeatedly isolated were used for further analyses (Supplementary Table 1a). Among them, we selected Olfr1019, Olfr57 and Olfr30 based on their high affinities for TMT measured by the cyclic AMP (cAMP)-induced luciferase assay6,17 (Supplementary Fig. 1c). Confirmation that these OR genes are indeed expressed in the dorsal OE was obtained by in situ hybridization (Supplementary Fig. 1f). Independent of this DiI screening, we analysed 266 previously reported ORs18 for their interactions to TMT by high-throughput screening in which TMT-responsive ORs were analysed not only in a posterior part of the DII subdomain but also in other areas of the OB (Supplementary Fig. 1d and Supplementary Table 1b). Combined with the three candidate ORs identified by the DiI experiment, a total of 18 ORs were further analysed for their dose responsiveness to TMT (Supplementary Fig. 1b). Six ORs demonstrating high TMT responsiveness were then subjected to ligand selectivity tests using 17 different odourants (Fig. 1c and Supplementary Fig. 1e). Among them, Olfr1019 showed the greatest specificity to TMT and was used for further gene-targeting experiments.
Knock-in and knockout mice for Olfr1019
For the loss-of-function and gain-of function experiments of the Olfr1019 glomeruli, we generated mutant mice in which the Olfr1019 gene was either deleted or linked to the coding sequence of channelrhodopsin (ChR) (Fig. 2a, and Supplementary Fig. 2a,b). For the knock-in (KI) experiment, ChR wide receiver (ChRWR) was used in place of conventional ChR2. ChRWR is a chimeric molecule of ChR2 and ChR1 and possesses advantages over ChR2, such as improved expression in the plasma membrane and enhanced photocurrent with smaller desensitization19. In the KI, ChRWR was fused with a fluorescent marker, Venus, so that OSN axons expressing the KI allele of Olfr1019 could be visualized. Venus-positive OSNs were all confined to the dorsal OE, and no Venus signal was found in the vomeronasal organ. As expected, a pair of fluoresced glomeruli was detected for Olfr1019 in the posterior DII subdomain of the OB (Fig. 2b,c and Supplementary Fig. 2c,d). Although the locations of Olfr1019-KI glomeruli varied among animals, they were primarily located within the posterior DII subdomain as identified by optical imaging upon exposure to TMT (Supplementary Fig. 2c). The KI glomeruli were rarely found in the lateral aspect of the dorsal OB where the 4MT-responsive glomeruli were clustered. We also analysed the knockout (KO) mice for the expression of the deleted Olfr1019 allele (Fig. 2c,d). Extended yellow fluorescent protein (EYFP)-positive OSN axons expressing the KO allele did not converge to a specific glomerulus, but were dispersed across the dorsal OB (Fig. 2d). When the OB sections were analysed by immunohistochemistry, OSN axons expressing the Olfr1019 KO allele were scattered and detected within various D-domain glomeruli. In situ hybridization revealed that OSNs expressing the Olfr1019 KO allele had activated another OR gene member to maintain the one neuron-one receptor rule4. These coexpressing secondarily chosen OR genes were not necessarily from the Olfr1019 cluster or from our gene list for TMT-responsive ORs (Fig. 2d).
Photoinduced spike-discharges in M/T cells
Mitral/tufted (M/T) cells are secondary neurons receiving odour signals from connecting glomeruli (Supplementary Fig. 3a) and send odour information to various regions in the olfactory cortex (OC)20,21,22,23,24,25. To determine whether stimulation of a single glomerular species could induce spike discharges in M/T cells, the KI mice were photoilluminated and action potentials of M/T cells were analysed by single-unit recording (Fig. 3a–c and Supplementary Fig. 3c). Layer locations of the tip of the recording microelectrode in the OB were examined by monitoring the configuration of lateral olfactory tract (LOT)-evoked field potentials (Supplementary Fig. 3a,b)9,24,25. We first studied the responsiveness of cells in the KI-OB to different dose intensities of photo exposure (Fig. 3b). In the external plexiform layer (EPL) unit, spike responses were saturated at 0.1 mW mm−2, whereas in the mitral cell layer (MCL) unit, responses were gradually increased and saturated at 1.0 mW mm−2. Consistent with a previous study demonstrating that tufted cells are more sensitive than mitral cells to lower concentrations of the TMT20, spike responses of the EPL unit were more sensitive to weaker light than the MCL unit (Fig. 3b). We then examined response profiles of M/T cells to different durations of stimulation with the light intensity of 1.3 mW mm−2 (Fig. 3c and Supplementary Fig. 3c). The EPL unit generated initial burst discharges of over 100 Hz immediately after the onset of 20 ms or longer durations of illumination (Fig. 3c and Supplementary Fig. 3c). In contrast, the MCL unit demonstrated spike responses to 50 ms or longer illumination (Fig. 3c). When durations of illumination were sufficient to induce spike responses, the photoilluminated cells yielded burst discharges at 50 Hz with delayed onset (Fig. 3c and Supplementary Fig. 3c). These results indicate that over 200 ms of photoillumination induces the plateau of spike rates in both the EPL and MCL units (Fig. 3a,c and Supplementary Fig. 3c).
TMT-responsive OC regions are partly activated by Olfr1019
To examine whether TMT-responsive brain regions are also activated by photostimulation of Olfr1019 glomeruli in the KI mouse, we studied the expression of Egr1, an immediate-early protein induced by neuronal activity, in the OB and in the following brain regions: anterior olfactory nucleus (AON), anterior piriform cortex, olfactory tubercle (OT), cortical amygdala (CoA), medial amygdala (MeA) and bed nucleus of stria terminals (BNST) (Fig. 4a–c and Supplementary Fig. 4a–h). To set up the condition of photoillumination, we examined Egr1 expression with three different illumination conditions: 2 Hz light in 20 ms pulses at 1.3 mW mm−2 for 30 min, 2 Hz light in 250 ms pulses at 1.3 mW mm−2 for 15 min, and 2 Hz light in 250 ms pulses at 1.3 mW mm−2 for 30 min. With the 20 ms stimulation, Egr1 signals were faint and difficult to detect (Supplementary Fig. 4b–h). In the experiment using the illumination condition of 250 ms pulses with 250 ms interval for 15 min, the result was basically the same as that with the stimulation for 30 min (Supplementary Fig. 4b–f). However, the signals were much weaker in the MeA and BNST (Supplementary Fig. 4g,h). We, therefore, chose to use the illumination condition of 250 ms pulses with 250 ms interval for 30 min. As a positive control, TMT (1 and 10%) absorbed onto a filter paper was presented to the wild-type (WT) mice (Fig. 4a–c and Supplementary Fig. 4b–h). We also analysed TMT-exposed Olfr1019-KO mice, vanillin-exposed WT mice and photoilluminated M72-ChR2-YFP mice26 for comparisons (Supplementary Fig. 4c–h). In the photoilluminated KI mice, Egr1 signals were found in periglomerular cells surrounding the Olfr1019 glomerulus and in the M/T cells right underneath (Supplementary Fig. 4b). In contrast, in the TMT-exposed WT mice, activated cells were detected in the broader region surrounding the Olfr1019 glomerulus (Supplementary Fig. 4b).
To identify the OC regions specifically activated in the photoilluminated KI mice, we compared the distribution patterns of Egr1 in the KI mice with those in the Olfr1019-KO and M72-Ch2-YFP mice (Fig. 4a–c and Supplementary Fig. 4c–h). Egr1 signals were found to be prominent in some cortical regions of the photoilluminated KI mice: in the AON, signals were notably high in the posterodorsal region (Fig. 4a, Supplementary Fig. 4c and Supplementary Movie 1); in the lateral OT, the anterior part of cap compartments was intensively labelled among three different components, cortical zone, clusters of interneurons in cap compartment and islands of Calleja (Fig. 4b, Supplementary Fig. 4e and Supplementary Movie 2). Previous tracing experiments revealed topographic correlations between the CoA and dorsal-region glomeruli that appear to be important in inducing innate aversive responses14,21. In the CoA and MeA, Egr1 signals were high in the anterior parts (Fig. 4c, and Supplementary Fig. 4f,g). In contrast, the signals were much reduced in these areas in the TMT-exposed KO mice (Fig. 4a–c and Supplementary Fig. 4c–g). The amygdalopiriform transition area (AmPir) was reported to be responsible for inducing stress responses27. Egr1 expression was not enhanced in the AmPir in the photoilluminated KI, although 10% TMT fully activated the AmPir in the WT (Supplementary Fig. 4f). In the BNST, Egr1 signals were high in the anterodorsal to medial regions in the photoilluminated KI mice. However, signals were high in the posterior region in the TMT-exposed WT mice (Supplementary Fig. 4h). In the TMT-exposed KO, Egr1 signals were reduced but still remained in the fear-responsive OC regions and in the BNST28 (Supplementary Fig. 4c–h). It is likely that in the KO, other TMT-responsive glomeruli remaining in the posterior DII subdomain had activated the fear circuit to compensate for the function of deleted Olfr1019 glomeruli.
Photoactivation of Olfr1019 glomeruli induces immobility
As photostimulation of Olfr1019-ChRWR glomeruli was able to activate TMT-responsive brain regions, we next examined whether fear responses could be induced in the photoilluminated KI mice. WT and KI mice were analysed in parallel for various types of behaviour (Fig. 5a,b,d and Supplementary Fig. 5b,d). The KI mouse whose head had been shielded with an aluminum foil was also analysed as a negative control. Mice (7–12 weeks old) were kept without light for the first 3 min of experiment, and then given optical stimulation. We first examined immobility of KI mice with the different pulse conditions (20, 50, 100 and 250 ms pulses at 1.3 mW mm−2) and intensities (250 ms pulse at 0.3 and 1.3 mW mm−2) of photoillumination (Supplementary Fig. 5a,e). Our results indicated that 250 ms pulse at 1.3 mW mm−2 intensity induced immobility more effectively than other pulse conditions. We then performed two sets of experiments with different durations of illumination. In one set, 5 s pulse was given every minute (Fig. 5a–d, Supplementary Fig. 5e and Supplementary Movie 3), whereas in the second set, 1 s pulse was given every 20 s (Supplementary Fig. 5b,d and Supplementary Movie 4). As shown in the video (Supplementary Movies 3 and 4), the KI mice demonstrated prolonged immobility responses by holding whisker movement and crouching the body down. In contrast, control mice (WT and shielded KI) continued to demonstrate various natural behaviours, for example, walking, stopping, exploring and grooming, despite photoillumination (Fig. 5a–c, Supplementary Fig. 5b,c and Supplementary Movies 3 and 4). To further exclude the possibility that photoactivation of any single glomerulus, regardless of the ligand specificity, could induce immobility responses, we analysed M72-ChR2-YFP mice26 in which the ChR2 gene was introduced into the M72 OR-gene locus. It has been shown that photostimulation of this mice generates sufficient signals in M72 glomeruli that can be transmitted to the central brain for memory-based go/non-go task26. As M72 glomeruli are localized to the dorsal surface of the OB, we photoilluminated and analysed M72 mice in the same manner as the KI mice of Olfr1019-ChRWR. We found that M72 mice did not show any immobility responses to photoillumination and behaved the same as the WT control (Supplementary Fig. 5e).
We then examined whether the photostimulation of Olfr1019 glomeruli in the KI mice of Olfr1019-ChRWR could cause any stress reactions. After photoillumination, adrenocorticotropic hormone (ACTH) levels were not so much increased in the KI as in the TMT-exposed WT mice (Supplementary Fig. 5g), indicating that photoillumination did not induce stress responses in the KI. This observation is consistent with the low Egr1 expression in the AmPir in the photo-illuminated KI mice but high in the TMT-exposed WT (Supplementary Fig. 4f). It is interesting, in this connection, that KI mice became immobile, but did not exhibit obvious avoidance behaviours upon photoillumination (Fig. 5d and Supplementary Fig. 5d). We also analysed Olfr1019-KO mice for TMT responsiveness (Fig. 5e,f, and Supplementary Fig. 5f). When subjected to 10% TMT, the WT demonstrated clear fear responses including immobility. However, the KO mice behaved differently upon stimulation with TMT, demonstrating lower immobility than that of the WT mice (Fig. 5e and Supplementary Fig. 5f). Interestingly, immobility responses towards TMT were not entirely abolished in the KO mice. This is likely due to the compensatory function of the remaining glomeruli in the posterior DII subdomain that are also TMT responsive. It should be noted that in this KO, aversive responses to TMT were not significantly affected (Fig. 5f).
In this study, we screened TMT-responsive ORs and identified Olfr1019 with high ligand affinity and selectivity. Our present data collectively demonstrate that stimulation of a single glomerular species for Olfr1019 is sufficient to elicit innate immobility. TMT usually activates multiple glomeruli and induces both avoidance and immobility responses in rodents10,11,12,14. In our optogenetic experiments, however, photoactivation of a single glomerular species for Olfr1019 induced immobility, but not clear aversion, indicating that this OR is specialized for only an immobility response. In this connection, Egr1 experiments demonstrated that photoactivated areas in the AON, OT, CoA, MeA and BNST were restricted to smaller areas compared with those activated by 10% TMT. Although the posterior regions of CoA are reported to be responsible for TMT-induced aversive responses, and AmPir is reported to be responsible for TMT-induced stress hormone responses14,27, these regions are less activated in the photoilluminated KI mice. These results are consistent with our behavioural analyses that the photoilluminated KI mice did not demonstrate aversive responses. It is likely that TMT-induced fear responses can be divided into at least two distinct components, immobility and avoidance, whose functional domains in the glomerular map may be separate. Because TMT activates not only the dorsal domain of immobility but also in other OB areas including the ventral region9,10, these nondorsal glomeruli may also contribute to the activation of OC, MeA and BNST.
Based on our previous studies2,8,9,10, we propose that the olfactory map is not merely a projection screen for pattern recognition of activated glomeruli but is also composed of functional subdomains for innate odour responses. As seen in the fly29, olfactory circuits in mice appear to be hard-wired for their innate responses. However, during evolution, the olfactory system could freely move out or move in a particular glomerulus from or to a specific functional domain in the OB by changing the expression levels of axon guidance molecules30,31. In this scenario, animals could easily adapt their olfactory system during evolution to the changes in the odour environment, such as appearance of new predators. It is generally thought that an activation pattern of a specific set of glomeruli is transmitted to the OC to discriminate and identify a particular odourant2,20,21,22,23. However, the olfactory system uses a different strategy to elicit innate fear responses than that of odour discrimination. Our present study clearly demonstrates that photoactivation of a single glomerular species for Olfr1019 is capable of inducing immobility responses in the ChRWR-KI mice, although multiple glomeruli are activated by TMT in both the dorsal and ventral regions. It appears that innate immobility could be induced once the M/T cells within the fear domain in the posterior DII region were activated even by a single glomerular species. Our KI mice will serve as an excellent model system to precisely identify the neural circuit from single glomeruli to specific areas in the brain regions.
Optical imaging and DiI staining
For optical imaging, odourants were presented to mice in a glass test tube after diluting them in mineral oil. For each recording trial, data were collected for 8 s with a frame length of 0.5 s (16 frames per trial). Each odourant was tested at least three times per animal. After imaging, 3% DiI in dimethylformamide was pressure-injected into the OB at the centre of an odour-activated glomerulus8,15,16. Odourants were obtained from Contech Inc., Sigma-Aldrich and Wako Pure Chemical Industries Ltd.
At 36 h after DiI injection, mice were anaesthetized, the OE was dissected and cells were dissociated with dispase (50 μg ml−1, Invitrogen) and collagenase (1 g ml−1, Invitrogen) at 37 °C for 10 min. DiI-stained cells were collected using a micromanipulator under a fluorescence microscope and used for single-cell PCR with reverse transcription5,15. To avoid contaminations, all samples were reexamined by PCR cloning. Primer sequences for PCR were 5′-ATGGCCTACGACCGGTACGTNGCNATHTG-3′ and 5′-TGGGAGGCGCAGGTGSWRAANGCYTT-3′.
Dual-Glo Luciferase Assay System (Promega) was used in our study6,17. We used CRE-luciferase (Stratagene) to measure an increase in the intracellular cAMP; Renilla luciferase driven by a constitutively active SV40 promoter (pRL-SV40; Promega) served as an internal control for cell viability and transfection efficiency. Hana3A cells were plated on poly-D-lysine-coated 96-well plates (BioCoat; Becton Dickinson). We transfected expression vectors for ORs with Rho tag in a Hana3A cell line along with vectors for RTP1S, CRE-luciferase and pRL-SV40 using Lipofectamine2000 (Invitrogen). Transfected cells were incubated with odourants for 12–16 h. The data were analysed using GraphPad Prism 4.
Gene targeting for the Olfr1019 was performed following the standard procedure32. Briefly, DNA fragments containing2 Olfr1019 were isolated from C57BL/6 BAC clones (RP23-384L16 BACPAC) and used for targeting. To generate KI (Accession No. CDB1182K) and KO mice (Accession No. CDB1008K: http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and KO mice, ires-ChRWR-Venus-loxP-neo-loxP and Tau-YFP-loxP-neo-loxP were inserted into the BAC clones just downstream and upstream of the Olfr1019 sequence, respectively. A 13 kb of DNA fragment with 5.2 and 3 kb of 5′- and 3′-arms was subcloned into the pBAD vector. The RED-ET recombination kit (Gene Bridges) was used for inserting the gene cassette and subcloning the targeting vector. The neomycin selection cassette was obtained from Gene Bridges (A003). The ChRWR plasmid of a C-terminal fusion construct with Venus was made as previously described19. M72YFP-ChR mice were purchased from the stock (021206) in the Jackson Lab26. Animal experiments were performed in accordance with the guideline of Animal Care Committees in the University of Tokyo, University of Fukui, Azabu University and RIKEN Kobe Branch.
Sixteen male mice (7–12 weeks old) were used for the single-unit recordings. For extracellular single-unit recordings from M/T cells, ∼1 mm diameter of the skull above the ChRWR-Venus-positive glomeruli was removed using fine forceps. A glass micropipette (50–100 MΩ) filled with 4 M NaCl was set right above or adjacent to the Venus-positive glomeruli20. Single-unit discharges were recorded from the EPL or MCL. Signals of single-unit discharges were amplified (MEZ-8301, Nihon Kohden, Tokyo, Japan), filtered (150-3 kHz; AB-610J, Nihon Kohden) and stored in a computer via an AD converter with the Spike2 software (Cambridge Electronic Design, Cambridge, UK). We identified one photoactivated M/T cell after 10–60 trials. We performed at most ∼20 trials for each glomerulus due to the damage caused by the electrode insertion.
The LOT-evoked field-potentials were recorded in the OB to determine the layer specificity of the cells24,25. A concentric electrode was inserted into the LOT (2.0 mm anterior to the bregma, 2.3 mm lateral from the midline and ∼5.5 mm from the brain surface) for electrical stimulation. Square current pulses (100 μs duration, 20–100 μA) were used to electrically stimulate the LOT24,25. Mitral cells were identified by their antidromic spike responses to the LOT stimulation.
Photo- and odour-induced Egr1 expression in the OB and OC
For the Egr1 histology experiments, 16 male mice (7–12 weeks old) were analysed. The photoillumination conditions are 20 ms pulse with 250 ms interval for 30 min, 250 ms pulses with 250 ms interval for 15 min and 250 ms pulses with 250 ms interval for 30 min. After photostimulation at the Olfr1019-KI glomeruli, animals were gently left for 60 min and transcardially perfused with saline followed by a fixative containing 4% paraformaldehyde. For odour exposure, mice were exposed to pieces of filter paper immersed with 1% TMT, 10% TMT or 10% Vanillin for 30 min, gently left for 60 min and perfused with 4% paraformaldehyde. Brains sections were prepared using a cryostat (HM500-M; Carl Zeiss) and immunostained by anti-Egr1 antibodies.
Immunohistochemistry and in situ hybridization
Anti-Egr1 and anti-GFP antibodies were purchased from Cell Signaling Technology (cat no. mAb4153) and Abcam (cat. no. ab13970), respectively. The secondary antibodies, Alexa Fluor 647-conjugated donkey anti-rabbit (Invitrogen A31573), Alexa Fluor 488-conjugated goat anti-chicken (Invitrogen A11039) and Alexa Fluor 555-conjugated donkey anti-rabbit (Invitrogen A31572) were used for visualizing signals at dilutions of 1:700 (anti-Egr1), 1:2,000 (anti-GFP) and 1:350 (Alexa Fluor antibodies), respectively. Frozen brain sections were incubated in primary and secondary antibodies overnight at 4 °C and for 2 h at room temperature, respectively. For OR expression in the nasal cavity, OE sections were analysed by in situ hybridization17. Signals were detected with the Tyramide signal amplification kit (Invitrogen Molecular probes TSA kit 40). All slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI) or Nissl.
Images of brain sections stained with anti-Egr1 and DAPI were collected using a fluorescence microscope, Model IX70 (Olympus), coupled to a cooled CCD camera, C4742-95-12ERG (Hamamatsu Photonics). Brain structures were identified microscopically and in digital photos according to the Paxinos and Franklin’s mouse brain atlas33. To count the number of Egr1+ neurons, Egr1-positive signals were distinguished from background by binarizing the images using a software, Adobe Photoshop.
Three-dimensional computer graphics for Egr1 expression
Pictures of fluorescence-labelled Egr1 expression in the 20 μm sections were taken every 100 μm, and volume rendering software (VCAT5), developed by the Image Processing Research Team at RIKEN, was used to incorporate the pictures and to process the images (http://logistics.riken.jp/vcat/vcat/en/). Detailed methodology and procedures for the imaging analysis by VCAT5 have been previously described34,35. To adjust the XYZ resolution, pictures with the value of pixel 0 were inserted into the blank space.
Behavioural analyses of freely moving KI mice
The 7- to 12-week-old male WT C57BL/6J and ChRWR-Venus KI mice were used for each experiment. At 2 days before the experiment, mice were anaesthetized with ketamine, followed by domitor or isoflurane. The skull above the dorsal OB was carefully thinned not to damage the OB surface with a handheld drill to achieve optical clarity. A custom-built head post was installed to gain access to the top of the skull for photo illumination. For the behavioural experiment, mice were put into a 20 cm × 17 cm transparent box with three cameras (SONY HDR-AS30V) on the top, side and front of the cage. Behaviour was observed for 3 min without photoillumination, and then a 473 nm wavelength of optical stimulation was delivered by an Omicron LuxX laser. A 500 μm plastic optical fibre (LUCIR COME2-DF1-1000) was set to sufficiently illuminate 1.3 mW mm−2 light on the dorsal OB. A 2-Hz 250 ms pulse width of 1.3 mW mm−2 light stimulation was given for 1 s every 20 s, or for 5 s every minute. As controls, mice whose heads were shielded with aluminum foil to block the light were analysed. Mouse behaviour was analysed with the software, Kinovea, running at a one-fifth reduced speed. AviUtl software was used for video editing. For aversion experiments, KI mice were placed in a 20 cm × 17 cm transparent two-chamber box. Photostimulation of 2 Hz for 1 or 5 s was given every 20 s in one of the two chambers. The time spent in the photostimulation room was measured within 30 min. For immobility experiments, two researchers independently analysed the video in blind and confirmed that the data are over 95% confident.
Odour-induced behavioural assay of KO mice
The 7- to 10-week-old KO and WT mice were placed in a 20 cm × 17 cm transparent box and their behaviours were observed for 10 min without odour stimulation. Mice were then given the odour stimulation with 100 μl of 1% or 10% TMT on 2 × 2 cm filter paper. The box was covered with a lid to fill the box with the odourants. For aversive behaviour experiments, mice were placed in 20 cm × 17 cm transparent two-chamber box. A piece of filter paper immersed with 10% TMT was placed in one particular room out of two in a cage. The time spent in the odour-exposure room was measured for the WT and KO mice within a period of 5 min. Data were analysed by GraphPad Prism 4.
Male WT C57BL/6J and ChRWR-Venus KI mice, 7 to 12 weeks old, were used for each experiment. After an optic fibre was connected to the head post, mice were left to acclimate for 2 h in clean cages. Then, 5 s photoillumination of a 2 Hz 250 ms pulses with a 10 s interval was repeatedly given for 10 min. For TMT exposure, WT mice were exposed to 10% TMT for 10 min. Mice were immediately decapitated. Plasma ACTH of each sample was analysed by the ACTH EIA kit (MD Bioproduct, cat. no. MO46006) and measured by the Bio-Rad Benchmark Microplate Reader (E15-1233).
The data that support the findings in this study are available from the corresponding author on request.
How to cite this article: Saito, H. et al. Immobility responses are induced by photoactivation of single glomerular species responsive to fox odour TMT. Nat. Commun. 8, 16011 doi: 10.1038/ncomms16011 (2017).
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This research was supported by Specially Promoted Research Grants (Hi.S.) and Grants-in-Aid (Ha.S., H.N., T.K. and K.M.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and grants from Urakami Foundation (H.N.) and Hayashi Memorial Foundation for Female Natural Scientist (Ha.S.). This work was also supported by the CREST Program (K.M.) and Japan-Israel Cooperative Scientific Research (Ha.S., K.M. and Hi.S.) sponsored by Japan Science and Technology Agency. We thank T. Ishizuka and H. Yawo (Tohoku U.) for providing us with ChRWR plasmid, A. Miyawaki (RIKEN Inst.) for Venus cDNA, K. Tamura (U. Tokyo) for his advice on laser illumination and S. Itohara (RIKEN Inst.) and his lab members for their advice on the behavioural experiment.
The authors declare no competing financial interests.
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Saito, H., Nishizumi, H., Suzuki, S. et al. Immobility responses are induced by photoactivation of single glomerular species responsive to fox odour TMT. Nat Commun 8, 16011 (2017) doi:10.1038/ncomms16011
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