Table 1: Details of the models used in this study.

From: Growth-coupled overproduction is feasible for almost all metabolites in five major production organisms

Organism (model name)Internal metabolitesReactions (operative)Repressible reactions (operative)Irrepressible reactions (operative)Substrate uptake limit (mmolgDW−1h−1)ATP maintenance demand (mmolgDW−1h−1)Reference
E. coli (iJO1366)1,8052,582 (1,761)1,414 (1,154)1,168 (607)15 (glucose)3.1526
S. cerevisiae (iMM904)1,2281,577 (1,217)1,020 (822)557 (395)10 (glucose)127
C. glutamicum (iJM658)9841,065 (647)788 (510)277 (137)5.4 (glucose)3.228
A. niger1,0371,280 (1,094)961 (877)319 (217)2 (glucose)1.929
Synechocystis sp. PCC 6803518594 (594)584 (584)10 (10)100 (photons)30
  1. ATP, adenosine triphosphate.
  2. Operative reactions are those that can carry a flux under the considered constraints and growth conditions (minimal medium). Irrepressible reactions (for example, exchange reactions, transporters, technical or pseudo reactions) are not allowed to be knocked out. The substrate for all organisms is glucose, except for Synechocystis sp. PCC 6803, which absorbs photons. ATP maintenance is a technical reaction that represents the ATP requirements for non-growth-associated maintenance processes. Where such a reaction was provided by the respective models, the lower limit of its flux is set to the value shown in the table.