Rapid identification of agronomically important genes is of pivotal interest for crop breeding. One source of such genes are crop wild relative (CWR) populations. Here we used a CWR population of <200 wild beets (B. vulgaris ssp. maritima), sampled in their natural habitat, to identify the sugar beet (Beta vulgaris ssp. vulgaris) resistance gene Rz2 with a modified version of mapping-by-sequencing (MBS). For that, we generated a draft genome sequence of the wild beet. Our results show the importance of preserving CWR in situ and demonstrate the great potential of CWR for rapid discovery of causal genes relevant for crop improvement. The candidate gene for Rz2 was identified by MBS and subsequently corroborated via RNA interference (RNAi). Rz2 encodes a CC-NB-LRR protein. Access to the DNA sequence of Rz2 opens the path to improvement of resistance towards rhizomania not only by marker-assisted breeding but also by genome editing.
Crop wild relatives (CWRs) are an invaluable genetic resource for crop improvement. CWR populations collected in their natural habitat not only provide access to agronomically important traits but can also serve to directly identify genes underlying these traits. Gene identification has been done so far mainly in three types of populations: germplasm collections, elite breeding materials, and synthetic populations such as biparental or multi-parent advanced generation inter-crossed populations1,2. Compared to those populations, CWR populations have usually undergone many generations of outcrossing and thus display low linkage disequilibrium and high population admixture.
Rhizomania is the most important sugar beet disease next to infection by beet cyst nematode (Heterodera schachtii). It is caused by the beet necrotic yellow vein virus (BNYVV) and reduces the yield of sugar beet by up to 80%. The BNYVV virus is transmitted by the soil-borne plasmodiophoromycete Polymyxa betae3. Starting from the first appearance in Italy in 1952, rhizomania has spread to almost all sugar beet-growing areas of the world4. The only way to control this soil-borne disease is through the cultivation of rhizomania-resistant varieties that have been made available since the mid-1980s (ref. 5). Resistance breeding has relied mostly on the resistance genes Rz1 and Rz2 as single loci or a combination of both6,7. The resistance conferred by Rz1 has been discovered in sugar beet germplasm, while Rz2 is derived from the wild beet Beta vulgaris ssp. maritima. Alleles that provide resistance show a dominant inheritance at both loci, and Rz2 confers a higher resistance level than Rz1 (ref. 8). Rz1 and Rz2 were mapped at a distance of about 20 cM on chromosome (Chr) 3 of the sugar beet6,9,10. A potentially additional rhizomania-resistance locus has been mapped to sugar beet Chr3 (BvChr3) and is most likely identical to Rz2 as it is derived from the same B. vulgaris ssp. maritima origin in Denmark11.
Here we demonstrate the potential of a CWR population for gene-level resolution mapping by identifying the sugar beet (B. vulgaris ssp. vulgaris) rhizomania-resistance locus Rz2 in a wild beet population of B. vulgaris ssp. maritima through a modified version of mapping-by-sequencing (MBS)12. The results not only confirm, in yet another case, the potential of CWR for crop improvement and the importance of preserving CWR in their natural habitats but also provide access to an agronomically important gene conferring resistance against rhizomania that encodes a CC-NB-LRR protein.
A CWR population to identify a candidate for Rz2 by MBS
Aiming at fine mapping and identification of the Rz2 gene, we sampled seeds from individual plants of the B. vulgaris ssp. maritima CWR population that is growing along the coast of Kalundborg, Denmark (Fig. 1). This population, referred to as Kalundborg population, is known to segregate for Rz2 (ref. 6). Given the allogamous flowering biology of this wild beet species, we assumed thorough population admixture and low linkage disequilibrium in the population, which are both features required for high-resolution mapping13.
We crossed 189 different parental wild beets with a rhizomania-susceptible sugar beet line and used the resulting 189 full sib families for a replicated progeny test. This test for rhizomania resistance consisted of four independent greenhouse assays, each including semiquantitative immuno-detection of BNYVV virions. The families showed segregation for rhizomania resistance and allowed inference of the genotype at the Rz2 locus of the respective parental wild beets (homozygous resistant (Rz2/Rz2), heterozygous (Rz2/rz2) or homozygous susceptible (rz2/rz2)). The results were explained by assuming a single locus, showing that Rz2 is the only rhizomania-resistance locus segregating in the Kalundborg population. Referring back to their original location, resistant genotypes appeared to be randomly distributed along the coastal line (Fig. 1, Supplementary Data 1). For most of the markers, the allele frequencies did not deviate significantly from Hardy–Weinberg equilibrium (HWE; Supplementary Table 1) and thus suggest a random mating population.
For MBS, we selected only parental wild beet individuals with high confidence in phenotype classification and used them to create one bulk of only four homozygous-resistant wild beets and one bulk with eight homozygous-susceptible wild beets. The two bulks were each sequenced using the Illumina technology (Supplementary Table 2). For access to a useful reference sequence for read mapping, the Rz2 donor accession WB42 (ref. 6, 7) was sequenced and assembled to draft quality (Supplementary Table 3). The assembly was concatenated according to the sugar beet reference sequence version 1.2 (ref. 14) to yield pseudochromosomes. RefBeet1.2 covers 567 Mbp of the B. vulgaris ssp. vulgaris genome that has an estimated haploid genome size of about 730 Mbp in nine chromosomes. The initial assembly, which was designated WB42-v1, was subsequently corrected manually with regard to the positioning of WB42 scaffolds and contigs to BmChr3 to yield the optimized assembly WB42-v2.
MBS analysis15 with WB42-v2 resulted in the detection of four closely connected intervals within a genomic region of 0.72 Mbp on BmChr3 (Fig. 2a,b, Supplementary Figs 1 and 2), overlapping with 25 almost continuous scaffolds and contigs of WB42-v2. Four smaller intervals spanning altogether about 0.09 Mbp at three loci were found on three other chromosomes (Supplementary Fig. 1, Supplementary Table 4). In the case of the scaffolds and contigs that cause signals outside of BmChr3, even detailed manual inspection did not allow us to deduce a reliable position in the genome sequence. However, mapping with additional markers indicated that these sequences also belong to BmChr3 but are not closely linked to the resistance locus defined by the phenotype (Supplementary Table 5). This excludes these three loci as possible location for Rz2. Gene prediction was carried out using the WB42-v2 assembly of B. vulgaris ssp. maritima together with RNA-Seq read evidence generated from tissue derived from rhizomania-infected plants related to line WB42. A total of 33,922 genes with expression evidence were predicted, including 60 genes in the target region on BmChr3. DNA and deduced peptide sequences of these 60 genes, and of 14 additional genes from the same region without expression evidence, were compared to the NCBI NR protein database and the current B. vulgaris annotation BeetSet-2 (ref. 16) (Supplementary Data 2). We detected a candidate for Rz2 that (i) encodes a CC-NB-LRR protein, (ii) is absent from BeetSet-2 obtained from the susceptible genotype KWS2320 and (iii) was supported by 100% transcript evidence in the WB42-v2 gene prediction. The Rz2 candidate had been included in the initial B. vulgaris gene prediction14 with the ID Bv3_jumg as a truncated gene but omitted from BeetSet-2 due to the absence of detectable transcription in the reference genotype KWS2320. Apart from its truncation in the reference sequence genotype, Bv3_jumg was located in a region that displays strong synteny between WB42 and KWS2320. The region was well assembled, co-linear and displayed no indication of read coverage deviation (Supplementary Fig. 3), indicating that no duplicated or paralogous loci from elsewhere in the genome interfered.
Fine mapping of the Rz2 candidate to rhizomania resistance
A total of 33 single-nucleotide polymorphisms (SNPs) segregating in the Kalundborg population, and distributed over the 0.72-Mbp target region, were converted to molecular markers (see Supplementary Table 6) and used to genotype the complete panel of the 189 parental wild beets (Supplementary Data 1). By association analysis of the marker results with the corresponding resistance phenotype data, 19 markers were significantly associated with resistance in at least two independent rhizomania-resistance tests (Fig. 2c; Supplementary Table 7). The best association was observed for marker CAU3880, which detected an SNP within the gene Bv3_jumg that encodes the CC-NB-LRR candidate for Rz2. Notably, CAU3880 was also the only marker that displayed association with rhizomania resistance (single marker F-test, P value=6.1E-4, r2=0.17) in an independent second CWR population that was sampled in Brighton, France (see Supplementary Data 3). Therefore, we hypothesized that the CC-NB-LRR gene Bv3_jumg containing CAU3880 is the best candidate for Rz2.
To validate this hypothesis, the markers with the highest association in the CWR populations were tested using diversity panels of sugar beet inbred lines representing the germplasm of three breeding companies (Supplementary Table 8). Across this association panel, only markers CAU3880, CAU4188 and CAU3882 showed perfect co-segregation with rhizomania resistance. In addition, detailed analysis of the recombination events detected in the Kalundborg CWR population showed that neighbouring genes upstream of Rz2 were excluded by CAU3881, while genes downstream of Rz2 were excluded by CAU4188. In the Kalundborg CWR population, the marker CAU4188 also excluded an ankyrin repeat encoding gene that is located downstream of Rz2 and included in BeetSet-2 (Bv3_tftt, see Supplementary Data 2). We concluded that Rz2 is the only gene that is fully associated with resistance to rhizomania.
Validation of Rz2 as the rhizomania-resistance gene by RNAi
To finally corroborate Rz2 (Bv3_jumg) as the rhizomania-resistance gene, an RNAi experiment was performed using the resistant sugar beet genotype 6921_RR. An RNAi construct was designed to inactivate the intact allele of Rz2KD-R, and this construct was transformed into 6921_RR. The transgenic line displays sensitivity to rhizomania to an extent comparable to the susceptible control (Supplementary Fig. 4).
Sequence analysis of the available Rz2 alleles revealed that alleles associated with susceptibility display defects in the CC-NB-LRR gene Bv3_jumg. The susceptible allele from the Kalundborg population (rz2KD-S) contained a premature stop codon, and the allele from the susceptible sugar beet reference genotype KWS2320 contained a transposon insertion (rz2KWS2320; Fig. 2d,e, Supplementary Table 9). We concluded that an intact allelic version of Bv3_jumg represents Rz2 and that susceptibility to rhizomania is caused by the absence of a functional allele of this gene. This conclusion is further supported by the similarity of Rz2 and the encoded CC-NB-LRR protein to other identified plant virus-resistance genes of the same type17.
The CC-NB-LRR family of the disease-resistance genes, which includes Rz2 on the basis of high amino acid sequence similarity, has been named after the domains that these resistance-conferring receptors typically contain: a nucleotide-binding site (NB) domain, a leucine-rich repeat (LRR), and a coiled-coil (CC) domain. CC-NB-LRR proteins can recognize a wide variety of taxonomically unrelated pathogens, including viruses, bacteria, fungi and even insects18. Activation of these genes results in a hypersensitive response characterized by the rapid death of cells in a local region surrounding an infection site, restricting the growth and spread of pathogens to other parts of the plant19.
The identification of Rz2 in a CWR population of an allogamous diploid species demonstrates the potential for high-resolution mapping of major genes. The small population size (N<200) drastically reduces the cost for phenotyping and marker assays. In comparison, when working with synthetic populations, 8,283 (ref. 20) and 5,457 (ref. 21) F2 plants were required for identifying sugar beet bolting time genes in a map-based cloning approach. In contrast to multi-parent advanced generation inter-crossed populations that required a minimum of eight crop generations2, it took only one step of seed increase to obtain a CWR-derived mapping population. Potentially, this step could even be skipped if the trait has high single plant heritability, such as bolting20,21 or other monogenic traits22. Although no direct comparison within B. vulgaris is available, the advantage of CWR populations over populations that are derived from germplasm collections or elite material13,23,24 is the high population admixture.
The confirmation of the correct identification of the candidate gene Bv3_jumg as Rz2 might have the complication that potentially existing additional sequence-related genes could be off-targets of the RNAi construct. However, read coverage analyses of the Bv3_jumg region as well as the correlation of the inactivated alleles (rz2) with susceptibility argue strongly against the existence of such genes in the B. vulgaris genome. Considering also the marker CAU3880 that is located within the candidate gene, we are confident that Bv3_jumg is Rz2.
Our approach relies on naturally occurring variation and is, therefore, independent of the production of a mutant population, unlike the recently published MutRenSeq method25. MutRenSeq combines chemical mutagenesis with targeted exome capture sequencing of resistance gene analogues (RGAs). The two approaches are distinct and complementary for the molecular identification of causal resistance genes. However, our approach also allows access to any other type of genes causal for a clear phenotype.
In the work presented, a CWR natural population was explored by application of MBS technology and allowed direct identification of a major locus of high agronomic importance. This was facilitated by the allogamous flowering biology of B. vulgaris ssp. maritima that led to an accumulation of recombination events over generations of reproduction. The process can be streamlined to fit into 1 year, including material development, phenotyping, sequencing and data evaluation given that sufficient personnel is available. Likewise, our approach could also be applied efficiently to other crops with allogamous CWRs, such as rye, sunflower and tomato26, highlighting the importance of protection and conservation of CWR27 in their natural habitats.
Natural population sampling and other plant material
Our study was based on a natural wild beet population located in Kalundborg, Denmark, that was reported to segregate for resistance to rhizomania6. From this CWR population, the rhizomania-resistant genebank accessions WB41 and WB42 had been collected and each had been used for mapping a major rhizomania-resistance quantitative trait locus in biparental crosses. The quantitative trait loci had been named Rz2 (WB42) and Rz3 (WB41) and had been mapped to the same genomic region. The mapping data also indicated that the Kalundborg population segregates for only one resistance locus. Therefore, we assumed Rz2 and Rz3 to be identical, referring to them as Rz2 (refs 6, 7, 28, 29).
Seeds were sampled in the coastal area of Kalundborg from 423 open pollinated wild beets and used for one seed increase. Single wild beet plants were grown each in isolation with plants of a cytoplasmatic male sterile sugar beet inbred line that was susceptible to rhizomania. Full sib family seed was harvested from the cytoplasmatic male sterile line plants, separately from the wild beet individuals that provided pollen. Seed production was carried out by the sugar beet breeding companies KWS SAAT SE, Syngenta Agro GmbH and Strube Research GmbH & Co. KG. A total of 189 full sib families with sufficient seed quantity and quality were selected for phenotyping, each derived from a different wild beet individual (Supplementary Data 1). About 40 offsprings were evaluated for each of the families to deduce the genotype of the respective Kalundborg parent individuals.
To obtain an independent population, 382 wild beets of a second CWR population were sampled in the coastal area of Brighton, France. This population has also been identified as a source for rhizomania resistance. Seed was increased as described above for the Kalundborg population by the sugar beet breeding company SESVanderHave N.V., and 129 full sib families derived from the Brighton population with sufficient seed quantity and quality were selected for resistance phenotyping (Supplementary Data 3).
For validation of the association of molecular markers in the Rz2 genomic region with resistance, a panel of 279 sugar beet inbred lines was used. This panel represented the breeding germplasm of KWS, Syngenta and Strube Research. The material covered rhizomania-susceptible and -resistant inbred lines that were phenotypically characterized for the status of Rz1 (ref. 6) and Rz2.
The full sib families derived from the Kalundborg population were phenotyped as follows: 10 single plants from each of 189 full sib families were evaluated by enzyme-linked immunosorbent assay (ELISA). Each ELISA plate held two samples of sap from healthy plants, plus samples for creating a standard curve. The standard curve was based on a series of dilutions: 400, 200, 100, 50, 25, 12, 6, 3, and 1 and, as the end point, a virus-free standard of 0 ng ml−1 was used. The 400-ng ml−1 standard was made from the sap of sugar beet roots with a high titre of BNYVV. The absolute virus content in that sample was determined using ELISA by comparing it to a purified BNYVV preparation control. Purified BNYVV and ELISA kits were bought from Adgen Ltd. in Scotland. The ELISA protocol described by the manufacturer was followed11. Phenotyping was carried out in four independent tests, with two tests using B-type and two tests using P-type BNYVV-containing soil30,31. The results are summarized in Supplementary Data 1. As controls, 100 plants of each of three different sugar beet lines were used in each test: an Rz2 line, an Rz1 line, and one rhizomania-susceptible line.
In addition, 129 full sib families developed from the Brighton population were phenotyped, as described above, in one test using B-type BVYVV30,31. As controls, 20 plants of an Rz2 line, an Rz1 line and one rhizomania-susceptible line were used in each test. The results are summarized in Supplementary Data 3.
Molecular marker analysis
Leaf samples were taken from each wild beet plant that was grown for production of full sib families. Extraction of genomic DNA was performed using the standard CTAB method32. For fine mapping, a total of 33 SNPs distributed within the target region identified by MBS of 0.72 Mbp in length were converted to Kompetitive Allele-Specific PCR (see Supplementary Table 6) or cleaved amplified polymorphic sequence markers and used to genotype the complete panel of 189 families. Kompetitive Allele-Specific PCR genotyping assays were performed in a total volume of 5 μl containing 0.1 ng of genomic DNA according to the manufacturer’s guidelines (LGC Genomics LLC, Beverly, MA, USA). Cleaved amplified polymorphic sequence genotyping was used for CAU4188 (WB42 allele: A; alternative allele: C; position in BmChr3: 8,694,501). An amplicon of 457 bp was amplified using the primers 5′-TGGAAGATTGTGCTGAGGAGA-3′ and 5′-GGACCTTCAGATGGCTTTGC-3′ by PCR. After PCR, an enzyme digestion using SspI was performed according to the manufacturer’s guidelines (ThermoFisher Scientific, Southampton, UK). Markers CAU4220, CAU4021 and CAU4022 (Supplementary Table 5) were evaluated by direct Sanger sequencing of amplicons generated by PCR.
For the markers in the 0.72 Mbp target region, deviations from the HWE expectations were estimated using the marker results listed in Supplementary Data 1. The P values for deviation from HWE are listed in Supplementary Table 1.
Genetic association analysis
Genotyping data from SNP markers targeting the 0.72 Mbp region on B. vulgaris ssp. maritima chromosome 3 (BmChr3) were used in the analysis. Association analysis for rhizomania resistance was performed in accordance with a Generalized Linear Model method using the TASSEL software (version 3.0). For assuming an association, an adjusted P value (Bonferroni correction) of <0.0015 was required, and r2 (correlation coefficient r2>0.1) was used to evaluate the magnitude of the marker effects. The results are summarized in Supplementary Table 7.
Generation of a WB42 draft genome sequence
A genome sequence of the Rz2 donor line WB42 was generated. DNA from a single plant was sequenced with the Illumina technology. Data generated from one paired-end (PE) library with an insert size of 600 bp and from two mate-pair (MP) libraries with span sizes of 2.5 and 5 kb, respectively, were combined. PE sequencing on an Illumina HiSeq-2000 instrument was performed using a 2 × 100 cycle protocol for the PE library and a 2 × 50 protocol for the MP libraries. Illumina sequencing reads were quality filtered and trimmed prior to assembly. Reads were removed if they did not pass the chastity filter, had at least one uncalled base, contained at least six bases of the Illumina adaptor sequence in both ends or had less than two-thirds of the bases with quality value Q≥30 within the first half of the read. Additionally, low-quality read ends marked as ‘B’ in the quality string (‘B-tails’) were trimmed off,14,33 resulting in 390 million high-quality PE read-pairs equivalent to 100-fold coverage of the beet genome. Quality filtering, trimming and redundancy elimination resulted in 30 million MPs for the 2.5 kb span size library and 35 million MPs with span size of 5 kb. Sequence read data were submitted to Sequence Read Archive (SRA). The reads were assembled using SOAPdenovo V1.05 (ref. 34) with standard parameters and a kmer-size of 49. Gaps were filled using SOAP GapCloser v1.12. The final assembly size was 531,940,822 bp in 57,361 scaffolds and contigs >500 bp. The largest scaffold had a length of 899,438 bp, and N50 size was 59,342 bp (see Supplementary Table 3). We refer to this initial assembly as WB42-v0 and to the 57,361 sequences in this assembly as ‘WB42-v0 contigs’.
Concatenation of contigs to yield WB42 pseudochromosomes
For MBS, we generated WB42 pseudochromosomes from the WB42-v0 assembly. Contigs were ordered according to the sugar beet reference genome sequence RefBeet-1.2 (ref. 14) assuming strong synteny between the two subspecies B. vulgaris ssp. vulgaris and B. vulgaris ssp. maritima. We used BLAST+ (ref. 35) to determine sequence similarity of the WB42-v0 contigs to RefBeet-1.2. The WB42-v0 contigs were sorted according to their RefBeet-1.2 mapping position as follows: (i) the WB42-v0 contigs were aligned to the reference with the dust filter turned off, an e-value cutoff of 1e-50 and a culling limit of 1; (ii) high scoring pairs were combined and the best overall alignment for each WB42-v0 contig was kept; (iii) WB42-v0 contigs were ordered by their mapping position; hits in minus orientation were included as reverse-complement, (iv) the contigs mapping to each chromosome were concatenated to pseudochromosomes with stretches of 50 N’s between contigs. We refer to this initial sorted and concatenated assembly as WB42-v1.
MBS was performed by applying a workflow15 that has been modified to fit the CWR setting, initially using WB42-v1 as a target genome sequence and an adapted interval detection (see below). MBS for Rz2 was based on the determination of allele frequencies in two DNA pools that were built from four resistant (R1/B2444) and eight susceptible (S1/B2446) individuals. Equal amounts of genomic DNA isolated from freeze-dried leaf samples32 of single plants were pooled according to the resistance phenotype and sequenced using the Illumina technology.
Library preparation and sequencing of pooled DNA
Library preparation for the DNA pools R1/B2444 and S1/B2446 was performed according to the Illumina TruSeq DNA Sample Preparation v2 Guide. DNA from each pool was fragmented by ultrasound shearing. After end repair and A-tailing, individual indexed PE adaptors were ligated to the DNA fragments, which allow a multiplexed PE sequencing run. The adaptor-ligated fragments were size selected on a 2% low melt agarose gel to a size of 350–650 bp. After enrichment, PCR of fragments of the final libraries that carry adaptors on both ends were quantified with a Qubit. The average fragment size of each library was determined on a BioAnalyzer High Sensitivity DNA chip. The samples were sequenced on a HiSeq-2000. Cluster generation for a high output run was done on a cBot using the TruSeq PE Cluster Kit v3, and 2 × 101 bp reads were generated using the TruSeq SBS Kit v3. Sequence read data of both pools were submitted to SRA. After completion of the sequencing runs, basecalling, demultiplexing and fastq file generation was performed using the CASAVA-1.8.2 programs. The results are summarized in Supplementary Table 2.
Targeted reordering of the WB42 sequence assembly
The allele frequency values (AFe) determined from sequence data (see below) of the two DNA pools indicated several genetic intervals with highly significant separation between the resistant (R1/B2444) and the susceptible (S1/B2446) pool. When using the WB42-v1 assembly as genome sequence, these intervals were located mainly on BmChr3 but in addition also on four other pseudochromosomes as well as in the unassigned assembly fraction. We concluded that the region of interest was assembled incorrectly and that the WB42-v1 assembly had insufficient quality for MBS and thus set out to improve the WB42 assembly to obtain a better mapping resolution. We focussed on BmChr3 for targeted reordering based on data from genetic markers that place Rz2 on BmChr3. We manually inspected scaffolds and contigs from RefBeet-1.2 that caused localization of WB42-v0 contigs to BmChr3 and analysed WB42-v0 contigs that might be incorrectly localized to other parts of the genome although the correct localization might be BmChr3.
We used data from a genotyping-by-sequencing experiment15 to identify true SNPs in regions of BvChr3 that displayed very low variation between the parents of the sugar beet mapping population that was used to build RefBeet-1.2 (ref. 14) and also data from BeetMap-3 (ref. 36). The regions that displayed very low variation between the parents turned out to contribute significantly to the wrong localization of contigs in WB42-v1. The respective RefBeet-1.2 scaffolds were addressed by marker-based genetic anchoring. Validated SNPs were genotyped by direct Sanger sequencing and manual SNP calling from the tracefiles in the KWS1 population36 and genetically anchored between 62.3 and 68.1 cM on BvChr3 (cM values according to BeetMap-3). Some sections within RefBeet-1.2 scaffolds were identified to be potentially misplaced based on sudden changes of AFe values in the corresponding WB42 scaffolds. To further verify the improved scaffold order on BvChr3, fosmid end sequences14,37 were evaluated, resulting in support for the proposed scaffold order. Additionally, sections of WB42-v0 scaffolds that (i) displayed a mean allele frequency in the R1 pool differing significantly from 0 (cutoff>0.05), (ii) corresponded to exactly one WB42-v0 contig and (iii) had a better BLAST hit elsewhere in the B. vulgaris reference sequence RefBeet-1.2 were moved to the position indicated by the best BLAST hit. The refined WB42 genome sequence assembly was designated WB42-v2.
Calculation of allele frequencies from sequencing data
All operations from postprocessing and mapping of reads over InDel realignment, base quality score recalibration to variant calling and calculation of AFe values were performed on the compute cluster of the CeBiTec. The reads of the sequenced pools R1/B2444 and S1/B2446 were processed by adapter trimming, quality filtered by removing reads with stretches of four consecutive bases with a mean quality value <30 and removal of bases at the read heads and tails with quality values <25 (ref. 15). The remaining reads were mapped to the ordered and concatenated WB42 sequences v1 and v2 using BWA-MEM. SNPs and InDels were called using GATK38. AFe and delta-AFe values were calculated from the proportion of reads for the two states of a given sequence variant within and between pools, respectively15.
Interval identification and prediction of causal variations
For visual inspection of the results, the delta-AFe values of detected variants were plotted along all WB42-v2 pseudochromosomes or for selected regions (see Fig. 2a and Supplementary Figs 1 and 2). To reduce background noise, only variants with a read coverage between 0.75 and 2.5 times the average coverage of uniquely mapped reads were plotted. Additionally, an established algorithm15 for automatic interval detection was adapted to the CWR scenario. An interval was defined as a genomic region containing a follow-up of variants with AFe values of ≤0.1 in the susceptible (S1/B2444) pool. Interruptions of the follow-up were ignored if they consist of at most 1 variation with an AFe >0.1, flanked on each side by a variant with AFe values of ≤0.1. Interval detection was started at seed variants with a delta-AFe value close to 1. The exact, lowest delta-AFe value of valid seeds (X) was calculated depending on the sequencing error rate and coverage to tolerate a small number of non-supportive reads. X=(P−100 × E/C) with phenotypic difference of the pools P=0.9, the coverage of both pools combined for the variant C and an estimate for errors introduced by HiSeq sequencing E=0.01. Adjacent intervals were merged into one if the gap between them was <1% of the length of the resulting interval after the merge. Finally, intervals <10 kbp were discarded. The resulting eight intervals detected in WB42-v2 were designated intervals 1–8 and are listed in Supplementary Table 5. Intervals 1–4 were located on BmChr3 and are shown in Fig. 2b and Supplementary Fig. 2.
Generation of RNA-Seq data from rhizomania infected beets
Selfing progenies of KD_091343, a rhizomania-resistant plant line derived from WB42, were grown in infected soil using the B-type BNYVV. Seeds were sown in sterile sand. Seven days after sowing, the seedlings were transplanted to 0.25-litre containers filled with a mixture of rhizomania-infected soil and sterile sand in 1:1 proportions. Before transplantation, the soil in the containers was thoroughly soaked with nutrient solution11. Root samples of KD_091343 were collected 3, 6, 9, 12, 15 and 20 days after transfer to the infected soil. The infection efficiency of the soil was confirmed by assaying plants of the rhizomania-susceptible line KD_091115, which were grown in parallel.
Total RNA was extracted from the infected root tissue using the peqGOLD Plant RNA Kit (PEQLAB) according to the manufacturer’s instructions. After on-column DNase treatment with the peqGOLD DNase I Digest Kit, the RNA was quantified. RNA-Seq (2 × 100 bp) was performed with TrueSeq technology on a HiSeq-1500 by using the Illumina RNA Sequencing Kit complemented with reverse transcriptase according to the instructions of the manufacturer. One barcoded library was created for each of the six time points. Raw RNA-Seq reads were processed as described39 and mapped to the WB42-v2 genome sequence (see below). In total, RNA-Seq yielded 177 million read pairs (335 million reads) with an average length of the single reads after trimming of 86 bp. Sequence read data of the six libraries were submitted to SRA. For expression detection and gene structure validation, the resulting BAM files were analysed with ReadXplorer2 (ref. 40).
WB42 ORF prediction
All scripts were provided by AUGUSTUS41 and used following AUGUSTUS’ instructions. Ab initio gene prediction on the WB42-v2 genome sequence was carried out using AUGUSTUS version 2.7 with the optimized sugar beet parameter set16 and extrinsic hints. The options to predict and print untranslated regions were switched on, the gene model was set to complete and no in-frame stop codons were allowed. Hint files were created based on RNA-Seq data derived from infected root material of KD_091343 (see above). Trimmed paired reads were used for a Tophat (v2.1.0)42 mapping allowing a maximum fragment length for valid PE alignments of 5,000. The BAM files were quality filtered to minimum mapping quality of 20. For merging and sorting, SAMtools43 were used. The resulting single BAM file was used as input for bam2hints.pl provided by AUGUSTUS to create intron part hints (option --I). To create exon part hints, BAM files were converted to WIG files using bam2wig and used to generate the hint file using wig2hints.pl. The structural annotation and information on transcript evidence of the 53,190 predicted WB42 genes is available in the file WB42_v2.gff3 (see below). Out of the 53,190 predicted WB42 genes, 33,922 displayed at least 1% transcript evidence.
Functional annotation of WB42 genes in intervals
The deduced 74 peptide sequences of the predicted WB42 genes located in the 0.72 Mbp target region on BmChr3 (60 with expression evidence and 14 without) containing the four main MBS intervals (see Supplementary Table 4) were analysed to collect functional annotation information for these genes. Note that the termini of the genetic intervals were determined by the position of variants within WB42-v2 scaffolds, which places these termini within scaffolds. As a result, the sum of the length of the scaffolds that overlap the four genetics intervals was longer than the total length of the four genetic intervals adding up to 0.55 Mbp (see Fig. 2b). For functional annotation, a BLASTp analysis of the WB42-v2 peptide sequences was carried out against the non-redundant protein sequence database (NCBI nr protein, update date: 2016/06/01 containing 88,499,796 sequences, E-value cutoff limit e-10), and functional annotation as well as the accession nos. of the best BLASTp hit were extracted. Second, the peptide sequences were analysed by BLASTp for their best hit in the set of all genes from RefBeet14, including those without transcript evidence (protein sequences extracted from RefBeet.unfiltered_genes.1302.gff3, which is available at http://bvseq.molgen.mpg.de/, E-value cutoff limit e-40). Third, sequences were used as query in a Blast Like Alignment Tool (BLAT) analysis44 against all peptide sequences deduced from BeetSet-2 (ref. 16). BLAT hits >50% query coverage are listed in Supplementary Data 2. BLAT hits with query coverage >80% and an identity of >85% were considered as an indication for the detection of true homologues of WB42 genes in BeetSet-2. Finally, we searched for known resistance gene domains in the WB42-v2 peptide sequences of the target region by using HMM models14 that had been established for sugar beet. A domain was considered as present if it was at least 95% complete with an E-value of ≤1e-5. We identified two RGAs; both RGA genes are highlighted in Supplementary Data 2. One of the two, namely, Bv3_073070_zfyr, has a true intact homologue in BeetSet-2 from the susceptible reference genotype KWS2320, whereas only one, Bm_jumg, is unique to WB42. The protein encoded by this WB42 gene, which received its designation in the initial B. vulgaris gene prediction14, contains the complete functional domains of a CC-NB-LRR protein (CC domain: cd14798; NB-ARC domain: pfam00931; LRR_8 domain: pfam13855).
Sequence analysis of Rz2 and rz2 alleles
For access to sequences of the Rz2 gene from different sources, we manually assembled the sequences of the gene from NGS read data and annotated the resulting sequences using CLC Main Workbench (version 6.9.1). The sequences of the two alleles from the Kalundborg population (designated Rz2KD-R and rz2KD-S, see Fig. 2e) were assembled from the sequence data of R1/B2444 and S1/B2446, respectively. The sequence of the allele rz2KWS2320 was taken directly from RefBeet-1.2 and required only re-annotation since the transposon insertion interfered with automatic structural gene prediction (see also Supplementary Data 2). The sequences of the rz2 alleles from YMoBv, YTiBv, KDHBv and UMSBv were constructed from their respective assembly data downloaded from http://bvseq.molgen.mpg.de/. Results are summarized in Supplementary Table 9. The presence of a premature stop codon or the insertion of a transposon were observed only in rhizomania-susceptible genotypes.
Verification of gene identification by RNAi
For a functional proof of Bv3_jumg, a resistant standard sugar beet genotype (line 6921_RR) was transformed with a DNA construct encoding a double-stranded hairpin RNA resulting in 6921_RNAi. The dsRNA was aimed at post-transcriptional gene silencing of the resistant Bv3_jumg allele. In order to provide a suitable DNA construct, a defined target sequence region of the resistant Bv3_jumg allele of 434 base pair length (nt 247–680 of the mRNA) was selected, amplified by PCR and cloned both in sense and antisense direction in the vector pZFN, a modified version of pLHBA, which is suitable for the synthesis of hairpin structures. This vector contains a double CaMV 35S promoter, two multiple cloning sites separated by intronic sequences, an intron from the Arabidopsis thaliana gene AtAAP6 and the nos terminator region. Transformation of sugar beet was performed in accordance with the established protocol45 and by using kanamycin as selection drug. Following a number of selection steps, successful transformation was examined on transgenic shoots via PCR by detection of the nptII gene, the AtAAP6 intron sequence, the two T-DNA border sequences (LB/RB) and the absence of vir. Positive shoots were clonally multiplied in vitro to 30 shoots in each case, rooted and transferred into soil in the greenhouse. Approximately 2 weeks later, the transgenic sugar beet plants (6921_RNAi) were transplanted into a rhizomania assay as described above. As controls, the non-transformed-resistant genotype (6921_RR) that was used for RNAi transformation and the susceptible line D108_ss were included (Supplementary Fig. 4).
The Illumina sequence read data were submitted to the NCBI SRA. Reads used to create the WB42-v0 assembly received accession no. SRP078074, and reads of the two DNA pools from the Kalundborg population received accession nos. SRX1922267 (R1/B2444) and SRX1940996 (S1/2446). Reads from the RNA-Seq experiment with infected root tissue received accession no. SRX1924131.
The contig and scaffold sequences that were used to build the WB42-v2 assembly were collected in FASTA format (WB42_v2.fasta). The sorting and concatenation of these sequences into pseudochromosomes for WB42-v2 is described in an AGP file (WB42_v2.agp). The three files WB42_v2.fasta, WB42_v2.agp and WB42_v2.gff3 are available from http://dx.doi.org/10.5447/IPK/2017/3.
The authors declare that all other data supporting the findings of this study are available from the corresponding authors upon request.
How to cite this article: Capistrano-Gossmann, G. G. et al. Crop wild relative populations of Beta vulgaris allow direct mapping of agronomically important genes. Nat. Commun. 8, 15708 doi: 10.1038/ncomms15708 (2017).
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This work was supported by the German Ministry of Food and Agriculture (BMEL) grant FKZ 2814505310 to F.J.K.-O. and M.V. and the German Ministry of Education and Science (BMBF) grant FKZ 0315957D (Acronym: NuGGET) to B.W. We thank Monika Bruisch for help with plant sampling, as well as Meike Pfeiler and Prisca Viehöver for excellent wet lab work. In addition, we thank Christian Jung for providing laboratory infrastructure and support.
The table summarizes data about details on geographical positions, resistance tests results (ELISA mean and standard deviation), and marker analysis results of individuals from the Kalundborg population. In addition, the column "MBS Selection" identifies the individuals selected for the MBS pools.
Results of BLAST and BLAT similarity analysis of Beta vulgaris spp. maritima WB42 protein sequences in the target region on BmChr3 to transfer functional annotation. The candidate gene for Rz2 is marked orange, genes on scaffolds "north" or "south" of the target region are marked grey.
Geographical positions and resistance tests results of 129 wild beet plants from the Brighton population.
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In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms
Plant Cell, Tissue and Organ Culture (PCTOC) (2018)