Understanding the function of the thousands of cellular proteins is a central question in molecular cell biology. As proteins are typically part of multiple dynamic and often overlapping macromolecular complexes exerting distinct functions, the identification of protein–protein interactions (PPI) and their assignment to specific complexes is a crucial but challenging task. We present a protein fragments complementation assay integrated with the proximity-dependent biotinylation technique BioID. Activated on the interaction of two proteins, split-BioID is a conditional proteomics approach that allows in a single and simple assay to both experimentally validate binary PPI and to unbiasedly identify additional interacting factors. Applying our method to the miRNA-mediated silencing pathway, we can probe the proteomes of two distinct functional complexes containing the Ago2 protein and uncover the protein GIGYF2 as a regulator of miRNA-mediated translation repression. Hence, we provide a novel tool to study dynamic spatiotemporally defined protein complexes in their native cellular environment.
Physical interactions between proteins are required for most cellular processes, and the identification and validation of protein–protein interactions (PPI) is a usual starting point when characterizing a novel protein. Various methods exist for the identification of potential PPI. A very common approach relies on the affinity purification (AP) of a bait protein from cell lysates, followed by a comprehensive identification of co-purifying proteins by mass spectrometry (MS). However, the analysis of such proteomics data is often complicated by the dynamic nature of protein complexes. Indeed, many proteins belong to multiple functional complexes with distinct or overlapping protein compositions. For instance, Argonaute (Ago) proteins, the central players involved in miRNA-mediated gene silencing1, are part of at least two functionally distinct complexes. In the miRNA-induced silencing complex (miRISC), Ago is involved in post-transcriptional repression of mRNA function, while in the RISC-loading complex (RLC), Ago gets loaded with miRNAs and interacts with factors stimulating this process. Multiple studies have performed AP-MS approaches using Ago as bait2,3,4. The corresponding data sets include proteins that play an important role at diverse steps of the pathway, such as the RLC components TRBP and Dicer, or the TNRC6 proteins that are core components of the miRISC. The AP-MS approaches, however, suffered from two main limitations: (1) important functional factors such as the CCR4/NOT complex, which is directly recruited by TNRC6 and is required for efficient miRNA-mediated silencing5,6,7 were notably absent from the AP-MS data sets, and (2) it is not possible to assign novel identified proteins to a specific step of the pathway as Ago is part of both RLC and miRISC.
To address the former, we decided to use the recently described BioID technique8. It is based on a variant of the E. coli biotin ligase BirA. BirA uses adenosine tri-phosphate and biotin to produce reactive biotinyl-5′-AMP that is tightly retained in its active centre, making it only accessible to a specific acceptor peptide. The BirA R118G variant, termed BirA*, has weaker affinity for biotinyl-5′-AMP allowing its release in the cytoplasm9, leading to the biotinylation of proximate proteins within an estimated 10 nm range10. Thus, fusion of BirA* to a bait protein enables biotinylation of vicinal proteins and their isolation on streptavidin-coupled beads. Side-by-side comparison of BioID- and AP-MS revealed that both methods identified relevant proteins but yielded moderately overlapping data sets due to the different bias of both techniques: AP detects rather stable interactions while BioID reflects close proximity within cells. Consequently, BioID proved to be better at detecting weak interactions or proteins with low expression levels11. With the different bias of BioID, we reasoned that it could be a viable alternative to identify additional PPI involved in miRNA-mediated silencing. However, since efficient biotinylation occurs over a time scale of 6–24 h (ref. 8), proteins identified by BioID integrate all potential interactions with the bait protein over this period of time. Hence, BioID-like AP-MS does not allow the analysis of specific complexes but rather gives an overview of all possible PPI in which a given protein may be involved.
A protein fragment complementation assay (PCA) is a powerful approach to validate known or putative binary interactions in a native cellular environment. Here a protein with a quantifiable activity is split into two poorly interacting non-functional fragments that can reassemble to restore activity when fused to two interacting proteins. We reasoned that a PCA based on BioID would be a unique assay for PPI that would address limitations of AP/BioID-MS approaches. Indeed, the conditional activation of BirA* on the interaction of two specific proteins allows harnessing the proximity-dependent labelling power of BioID in a much more spatially and temporally defined manner with the potential of selectively biotinylate-specific subcomplexes. We show here that it is indeed possible to split BirA* into two PCA-suitable fragments. The resulting split-BioID assay is a conditional proteomics method that allows validating in a single and simple assay binary PPI, and the concomitant labelling of additional vicinal proteins belonging to the corresponding complex in live cells. Using various examples, we demonstrate that our split-BioID technique is a bona fide PCA. Furthermore, focusing on PPI involved in the miRNA-mediated silencing pathway, we show that it allows very-high-resolution proteomics of functional complexes. Indeed, by selectively activating Ago with either Dicer or TNRC6 using split-BioID, we could specifically analyse the RLC or the miRISC and identify a previously unknown regulator of miRNA-mediated translational repression. Another study recently described an alternative split-BioID assay12, suggesting that BirA* can be split at multiple sites.
Design of split-BioID
To develop our split-BioID PCA, it was essential to identify two inactive fragments of BirA* that can reassemble into an active enzyme when brought in close proximity (Fig. 1a). To this end, we made use of the FKBP (12-kDa FK506-binding protein) and FRB (FKBP-rapamycin-binding domain) proteins that do not interact in the absence of rapamycin but form a tight ternary complex in its presence13. The E. coli BirA enzyme is made of three subdomains (Fig. 1b): an N-terminal domain interacts with DNA and mediates repression of the biotin operon, a central part binds biotin and contains the catalytic site14, a C-terminal domain interacts with the natural substrate protein and contributes to adenosine tri-phosphate binding15. According to published data, we split BirA* at three different sites, and the corresponding N-terminal (NBirA*) and C-terminal (CBirA*) fragments were fused to FKBP and FRB, respectively (Fig. 1a,b). Two constructs (N270/F271 and N273/R274) split BirA* in a way that separates the C-terminal domain that was previously described as essential for the activity of BirA15. Another construct segregates the N-terminal DNA-binding domain (Q65/L66), which was also reported to stimulate activity16. In addition, a fourth construct (E256/G257) was designed guided by structural analysis of the enzyme and splits BirA* before the last helix of its core catalytic domain. The corresponding protein fragments were then co-expressed in HeLa cells, and biotinylation analysed in the absence or presence of rapamycin (Supplementary Fig.1). The best results were obtained for the E256/G257 splitting site that produced two fragments with low activity, as demonstrated by low levels of biotinylation in the absence of rapamycin, that were efficiently reactivated on rapamycin-mediated interaction. This splitting site was thus used for all further experiments. Conveniently, NBirA* and CBirA* can be indifferently appended to the N- and C termini of FRB or FKBP as all combinations of fusion proteins tested (Fig. 2a) led to strong activation of biotinylation on addition of rapamycin (Fig. 2b,c). This suggests that split-BioID can be applied to any proteins provided they can be tagged either on their N- or C termini. Typical for a PCA, the re-assembled fragments showed a reduced relative activity (ca. 2.5%) when compared to BirA* (Fig. 2d). This lower activity was sufficient for performing BioID experiments (see below). A recent study also described a PCA based on two BirA* fragments using an alternative splitting site (E140/Q141)12. We wondered if this splitting site would perform similar to our PCA. To test this, we compared both split-BioID assays using our FRB/FKBP system. With this side-by-side comparison, our splitting site appears to perform better as it resulted in a much stronger re-activation on rapamycin-induced dimerization evidenced by the observed stronger biotinylation signal with similar expression levels of the NBirA* and CBirA* fusions (Supplementary Fig. 2).
Monitoring a phosphorylation-dependent complex
Split-BioID was next validated on a physiological phosphorylation-dependent PPI that had been previously analysed with a split luciferase PCA17. Throughout the cell cycle interphase, the G2/M transition-regulating protein phosphatase Cdc25C is phosphorylated at S216, and subsequently binds 14-3-3ɛ. This binding sequesters Cdc25C in the cytoplasm, impeding access to its nuclear substrates18. We fused CBirA* with either Cdc25C WT or an S216A non-phosphorylatable variant, and fused NBirA* with either the Cdc25C WT-binding partner 14-3-3ɛ or green fluorescent protein (GFP) as a negative control. Transient co-expression of CBirA*-Cdc25C WT with NBirA*-14-3-3ɛ yielded a much stronger global biotinylation signal than the Cdc25C S216A/14-3-3ɛ combination (Fig. 3a,b), similar to the split luciferase PCA that was performed on the same proteins17. As expected, the Cdc25C WT/GFP pair produced only background levels of biotinylation. Importantly, immunofluorescence experiments showed that the fusion proteins are all localized in the cytosol (Fig. 4). Of note, we observed that NBirA*-14-3-3ɛ was much less expressed than endogenous 14-3-3ɛ, while CBirA*-Cdc25 fusion proteins were overexpressed when compared to endogenous Cdc25C (Supplementary Fig. 3). The same was true when stable cell lines were constructed using the same plasmids that were used for transient expression, albeit both fusion proteins were expressed at lower levels (Supplementary Fig. 3).
Although, the sequestering role of the 14-3-3ɛ/Cdc25C interaction may imply that no additional protein should be bound by this pair, we next performed MS analysis and label-free quantification (LFQ) on biological triplicates of the Cdc25C WT/14-3-3ɛ and Cdc25C S216A/14-3-3ɛ samples from stable cell lines. Significant enrichment was calculated over a pool of BioID runs on six unrelated proteins (see ‘Methods’ section) and we further deleted the resulting hits that had LFQ values lower than in the split-BioID GFP control sample. We then look at proteins enriched in the Cdc25C WT/14-3-3ɛ over the Cdc25C S216A sample. Nine such proteins were found (Fig. 3c and Supplementary Data 1). Consistent with the gain in affinity for 14-3-3 proteins of phosphorylated Cdc25C compared to the non-phosphorylated form (dissociation constant of 57 nM and 1.2 μM (ref. 19), respectively), 14-3-3ɛ was enriched ca. fourfold and Cdc25C 40-fold in the WT sample. The other seven proteins have no obvious link to the function of Cdc25C but we nevertheless tried to validate two potential interactions (CKAP5 and LMO7) with Cdc25C and 14-3-3ɛ by co-IP experiments. While we could not find evidence for an interaction with CKAP5, LMO7 was co-immunoprecipitated with (myc-tagged) NBirA*-14-3-3ɛ and with (FLAG-tagged) CBirA*-Cdc25C (Fig. 3d). Interestingly, during the revision of this paper, a study described an interaction of Lin11, Isl-1 and Mec-3 (LIM) proteins, including LMO7, with Cdc25C and 14-3-3ɛ. In this study, LIM proteins were shown to both positively regulate phosphorylation of Cdc25C and promote sequestering of Cdc25C by forming a ternary complex with Cdc25C and 14-3-3ɛ in the cytoplasm20. Hence, split-BioID correctly identified LMO7 as interacting with the phosphorylation-dependent Cdc25C/14-3-3ɛ dimer. It will be interesting to see if some of the other identified proteins are also previously unknown components of the cytoplasmic Cdc25C/14-3-3ɛ complex. Finally, it is noteworthy that, as predicted, the nuclear targets of Cdc25C, Cdc2 and cyclin B21 are absent from the MS data. Together, these data demonstrate that split-BioID is a bona fide PCA that can validate binary PPI under different conditions.
Monitoring maturation of the miRISC
We next explored the potential of the method for resolving functional subcomplexes involved in miRNA-mediated gene silencing. In the course of the formation of the miRISC, Ago is successively part of two distinct protein subcomplexes containing either Dicer or TNRC6 (Fig. 5a). Dicer processes pre-miRNAs to produce miRNAs that are then loaded onto Ago. When bound to Dicer, Hsp90/Hsc70-stabilized22 empty Ago is part of an RLC that contains a handful of proteins23. The Hsp90/Hsc70 machinery not only stabilizes empty Ago2, but also actively participates in stimulating the transfer of miRNAs to Ago24. Once loaded with miRNAs, Ago directly interacts with TNRC6 proteins to form the miRISC that represses translation and stimulates mRNA decay1, and partially localizes to RNA granules25,26. We analysed whether split-BioID could discriminate between these two steps of miRISC assembly. We first tested different fusion proteins of Dicer, and the Ago and TNRC6 paralogs Ago2 and TNRC6C (Fig. 5b and Supplementary Fig. 4a). We found that, when combined to various NBirA* fusion proteins, CBirA* fused to GFP consistently produced significant biotinylation signals that precluded its use as a negative control (Supplementary Fig. 4b). This is in contrast to CBirA* fused to CNOT8 (an indirect interacting partner of Ago2, used as a negative control for the PCA) that yielded background biotinylation signal when combined with NBirA*-Ago2. As a possible explanation, we noticed that CBirA*-GFP was expressed at much higher levels than any other fusion protein we used in this study. This might drive re-association with the co-expressed NBirA* fusions and lead to increase background. By contrast, a CBirA*-Ago2 fusion led to strong activation when combined to NBirA* fused to either Dicer or TNRC6C, and to background signals when combined to NBirA*-GFP (Fig. 5b). Hence, this combination was identified as the best and was used further. When compared to the endogenous proteins, we observed that CBirA*-Ago2 was less expressed than endogenous Ago2 while NBirA*-Dicer was clearly overexpressed (Supplementary Fig. 4c). The comparison could not be performed for NBirA*-TNRC6C as full-length TNRC6C does not seem to be expressed at significant levels in HeLa cells, which agrees with functional knockdown data performed on the TNRC6 paralogs in HeLa cells27.
Split-BioID was then performed on transiently transfected cells and the resulting biotinylated proteins were isolated. To test for the general specificity of split-BioID, NBirA*-TNRC6C was immunoprecipitated. We observed that, in addition to CBirA*-Ago2, interaction-induced biotinylated proteins were also found in the precipitated material but not in the control IP (Fig. 5b), strongly suggesting that these proteins are bona fide interaction partners of TNRC6C or Ago2. To determine the resolution of the method, known components of the two different Ago-containing complexes, RLC and miRISC, were probed. TAR RNA-binding protein (TRBP) is a cofactor of Dicer that stimulates miRNA loading onto Ago2 within the RLC28,29, while the CCR4/NOT complex is recruited to the miRISC through direct interaction of TNRC6 with the CNOT1 subunit5,6. Strikingly, TRBP was specifically detected in the Ago2/Dicer split-BioID, while CNOT1 was only detected in the Ago2/TNRC6 split-BioID (Fig. 5c). Both proteins were absent from the Ago2/GFP-negative control sample as well as in the control samples where NBirA*-Dicer and NBirA*-TNRC6C were expressed in the absence of a CBirA* fragment, excluding residual biotinylation activity of both fusion proteins. Hence, in addition to validating binary interactions, split-BioID is also able to specifically identify additional PPI belonging to the corresponding subcomplexes. Finally, IF experiments showed that all fusion proteins were mainly localized to the cytosol and not segregated to different compartments (Fig. 6).
Probing the RLC and miRISC proteomes
MS analysis was then performed on biological triplicates of the split-BioID samples, significant enrichment was calculated over a pool of BioID runs on six unrelated proteins (see ‘Methods’ section) and over the split-BioID GFP control. Altogether, 68 proteins were enriched over control samples (Supplementary Data 2). We then looked at LFQ enrichment in the TNRC6C sample over the Dicer sample (Fig. 7a and Supplementary Data 2). The TNRC6C-enriched proteins (50 proteins, 40% known to be physically or functionally Ago2-associated, including 32% proposed to have miRISC-associated function) excluded RLC-associated components while miRISC-associated factors (Fig. 7b and Supplementary Data 2) were absent in Dicer-enriched proteins (14 proteins, 8 known to be physically or functionally Ago2-associated, including 7 proposed to have a RLC-associated function). As predicted, Dicer is absent from the Ago2/TNRC6C sample while TNRC6C is excluded from the Ago2/Dicer sample. As a comparison we generated a data set of potential interacting proteins by applying classical BioID to Ago2. Similar to AP-MS, BioID-MS (Supplementary Data 3) identified components of both RLC and miRISC including Dicer and TNRC6C. Hence, split-BioID considerably increases the resolution of the assay. Interaction network analysis shows that BioID identified PPI clustering around two main hubs (Supplementary Fig. 5). Hub1 is centred on the polyA-binding protein (PABPC) and comprises miRISC components. Hub2 is centred on Hsp90 proteins and comprises RLC components. When combined, the proteins identified from the Ago2/Dicer and Ago2/TNRC6C split-BioID show a similar network of PPI clustering around the same two main hubs (Supplementary Fig. 5a).
When analysed separately, Ago2/TNRC6C-enriched proteins comprised proteins of Hub1, coherent with the functional role of this complex as a regulator of mRNA function. Consistently, gene ontology analysis for these proteins showed that the most enriched terms are poly(A) RNA binding, ribonucleoprotein granule and post-transcriptional regulation of gene expression (Supplementary Fig. 6a). Ago2/TNRC6C split-BioID identified core components of the miRISC that were present (PABPC, DDX6) or absent (such as subunits of the CCR4/NOT complex) in AP-MS data sets (Fig. 7c). The two other TNRC6 paralogs, A and B, were the most abundant proteins detected by split-BioID, this may reflect the fact that mRNAs often harbour multiple miRNA sites and thus can be bound by multiple miRISC complexes that may come in close proximity, alternatively TNRC6 proteins might bind Ago as multimers. In addition, proteins that regulate miRISC action (such as RC3H2 (ref. 30), FMR1 (ref. 31) and ATXN2L (ref. 32)) were also identified. Interestingly, an importin (TNPO1) was found in this data set, which is in line with data showing that TNRC6 navigates Ago into the nucleus of mammalian cells33,34. Additional factors with no previously known direct connection to the miRNA pathway comprise several classes of proteins. Half of these proteins are RNA-binding proteins (RBPs) and thus some of them might be found in proximity to the miRISC because they bind to common target mRNAs. Yet, some of these RBPs are known to directly interact with miRISC-associated factors (for example, PATL1 binds to DDX6 (ref. 35) and HELZ binds to CNOT1 (ref. 36)) and thus may play a role in miRNA-mediated silencing. The other proteins may just reflect the direct proximal cellular environment of the miRISC without necessarily having any role in the miRNA pathway or may represent novel miRISC-associated factors.
Conversely, the Dicer-enriched proteins comprised proteins of the Hub2. The most enriched gene ontology terms are heat shock protein binding, cytosol and protein refolding (Supplementary Fig. 6b). Out of the 12 proteins identified (excluding Dicer and Ago2), eight are components of the Hsc70/Hsp90 chaperone machinery (Fig. 7c). The Hsc70/Hsp90 machinery was shown to stabilize empty Ago2 (ref. 22) but also to have an evolutionary conserved role in stimulating efficient miRNA loading37,38,39,40,41,42. Hence, by analogy to the role of the same chaperone machinery for the activation of steroid receptors43, Hsc70/Hsp90, and a cohort of co-chaperones, are proposed to actively participate in the process of miRNA loading into Ago24. The identity of the co-chaperones assisting Hsc70/Hsp90 in loading Ago2 has remained elusive. Split-BioID identifies five of them (Fig. 7b). While Cdc37 (ref. 39) and Hop (STIP1)44 were already shown to affect RISC loading, PP5 (PPP5C) was shown to associate with plant Ago1 and Hsp90 (ref. 37). RPAP3 acts as an Hsp90 co-chaperone within the R2TP complex, which helps the assembly of ribonucleoprotein complexes45 but a connection with miRISC assembly has not been described yet. The same goes for the last identified co-chaperone, UNC45A. Both will be subject of future studies.
Other previously found Ago2-associated chaperones, such as Fkbp4/5 and p23 (refs 38, 39), as well as the RLC proteins TRBP28,29 and PACT46 were absent from the Dicer-enriched data set. Similarly, not all subunits of the CCR4/NOT complex were found in the TNRC6C-enriched data set. One possible explanation may be that these proteins were out of the labelling range of split-BioID or may not have accessible acceptor lysine residues for biotinylation. Another explanation may be the detection limit of the assay. In the Ago2/Dicer sample, TRBP was detected by western blot. Conversely, PACT and CNOT2 were detected by MS but showed enrichment below our significance threshold. Given the exceptional strength of the streptavidin/biotin interaction, directly performing trypsin digestion on the streptavidin-coupled beads rather than first eluting biotinylated proteins, as we did, may lead to deeper identification coverage. Nonetheless, split-BioID allowed us to specifically probe the proteomes of the miRISC and RLC.
Identification of a novel miRISC-regulating factor
We then asked whether split-BioID could identify novel factors associated with the miRNA pathway. As we are interested in novel regulators of the miRISC and the Ago2/TNRC6C split-BioID yielded more unknown proteins, we focused on this data set. We decided to study the protein GIGYF2 as previous data indicated that it may be part of a translation repression complex47. GIGYF2 was previously neither found as associated with Ago2 or TNRC6 proteins by AP-MS nor described to play a role in miRNA-mediated repression. We first analysed by western blot, the presence of GIGYF2 in the split-BioID samples. As it was clearly detected in the Ago2/TNRC6C but not in the Ago2/Dicer sample (Fig. 5c), split-BioID assigns GIGYF2 to the miRISC rather than the RLC. Immunofluorescence microscopy was performed to detect endogenous Ago2 and GIGYF2, while Ago2 was detected both in the nucleus and cytosol as previously reported48, GIGYF2 was mainly found in the cytosol where it partially co-localized with Ago2 (Fig. 8a). To address if Ago or TNRC6 interact with GIGYF2, co-IP experiments were performed. For these experiments, the TNRC6A paralog was studied due to the availability of suitable antibodies. Endogenous Ago2 and TNRC6A could be detected in immunoprecipitation performed with antibodies directed against endogenous GIGYF2 (Fig. 8b). RNAse treatment abolished interaction with Ago2 but a weak signal was still observed with TNRC6A. However, no signal for GIGYF2 was observed in the reverse co-IP performed with an antibody directed against Ago2 while a very faint but reproducible signal was observed in the TNRC6A immunoprecipitation (Fig. 8b). Altogether, these weak signals may explain why GIGYF2 was never found in AP-MS approaches applied to Ago2. TNRC6 proteins harbour a proline-proline-glycine-leucine (PPGL) sequence, which is a typical recognition motif for GYF domains. Accordingly, TNRC6A has been found in pull-down experiments performed with the isolated GYF domain of GIGYF2 fused to GST49. To test if the PPGL motif of TNRC6C mediates a direct interaction with the GYF domain of GIGYF2, we expressed recombinant maltose-binding protein (MBP)- and His6-tagged fragments corresponding to the C-terminal effector domain (CED) of TNRC6C (ref. 6) harbouring the PPGL motif, or an AAGL variant thereof (Fig. 8c). In addition, we expressed two GST-tagged fragments of GIGYF2: (532–740) comprised the GYF domain while (607–740) did not (Fig. 8c). Strikingly, after mixing and immobilization on Ni-NTA beads, MBP-CED-WT-His6 could be co-eluted with GST-GIGYF2 (532–740) comprising the GYF domain of GIGYF2 (Fig. 8d). Binding was considerably reduced on mutation of the PPGL motif to AAGL within the CED, or when the (607–740) fragment of GIGYF2 without GYF domain was used. Altogether, GIGYF2 shows an interaction with Ago and TNRC6, mediated by a direct interaction of the PPGL motif of TNRC6 with the GYF domain of GIGYF2. Within the cellular context, this interaction seems stabilized by RNA and is probably transient as it is well detected by split-BioID but hardly by co-IP. We next tested if GIGYF2 modulates the activity of miRISC. We and others50,51,52 have previously shown that miRNA-mediated repression is established through three successive steps: translational repression, followed by mRNA deadenylation, which is tightly coupled to mRNA decay. Previously, we have described inducible cell lines expressing luciferase reporters of miRNA action that allow studying either the translation repression or the mRNA decay component of miRNA-mediated repression. Indeed, when the reporters are analysed 2 h post induction, they are essentially repressed at the translation level, while at later time points mRNA decay mostly accounts for the observed repression51. Using a pool of specific siRNAs, GIGYF2 was depleted from these cell lines (Fig. 9), and luciferase activity was analysed at various time points (Fig. 9a). Strikingly, at 2 h post induction, miRNA-mediated repression was alleviated while at later time points repression was unaffected. By contrast a knockdown of the TNRC6 proteins that are necessary for all steps of miRNA-mediated silencing alleviates repression at all time points, while knockdown of subunits of the CCR4-NOT complex (involved in the mRNA destabilization steps and partially in the translation repression steps) also affects the later time points.
Altogether, split-BioID correctly identified GIGYF2 as a miRISC-associated factor and allowed identifying a novel regulator of miRISC activity, at least for the reporter we studied. Our data suggest that GIGYF2 directly and transiently associates with TNRC6 proteins and specifically favours the translational repression component of miRNA-mediated silencing but does not modulate mRNA decay. Interestingly, in zebrafish, a conserved PPGL motif within TNRC6A had been described as mediating translational repression independent of mRNA deadenylation and decay53. This has led to the hypothesis of a yet-to-discover factor that binds to TNRC6 proteins through this motif54. Our data reveal that GIGYF2 is that missing factor and further experiments will aim at deciphering its precise mechanism of action. In that vein, a split-BioID applied to the TNRC6/GIGYF2 pair will be of upmost interest.
In summary, we have shown that it is possible to split BirA* in two PCA-suitable fragments. Like other already available PCAs, Split-BioID can be used to validate binary interactions in their native cellular environment. Split-BioID is rather not suited for a high-throughput assay; however, the innovation that singles it out when compared to other PCAs is the possibility to identify additional factors associated with the pair of interacting proteins. As biotinylation of vicinal proteins only happens when and where two proteins interact, split-BioID is a conditional proteomics approach that identifies spatially and temporally defined dynamic complexes. Global proteomics approaches for identifying protein complexes, such as protein correlation profiling55 or co-elution profiling56, were successfully applied to get a general overview of cellular protein complexes. These approaches are particularly strong at identifying complexes with defined localization and composition, however they seem to fail at identifying dynamic complexes as many centrosomal proteins were missed by protein correlation profiling and co-elution profiling failed to identify the miRISC or CCR4/NOT complexes, possibly because both methods require cell lysis and lysate fractionation. Split-BioID is an ideal complement to these global approaches as it focuses on specific dynamic complexes in their native cellular environment. A potential limitation of the approach comes from the speed of labelling. In BioID, efficient biotinylation has been reported to require 6–24 h. Though the conditional activation of split-BioID ensures it labels proteins involved in temporally defined interactions, proteins with very short half-lives will likely be difficult to detect. Identifying a more active variant of BirA* or designing a PCA based on the engineered peroxidase APEX2 that mediates strong biotinylation within a minute57 may circumvent this limitation.
Another potential caveat is the level of expression of the fusion proteins. We have co-expressed NBirA* and CBirA* from the same plasmid both under inducible CMV promoters. This has resulted in having one fusion protein being more expressed than its endogenous counterpart. In this study, we focused on establishing the technique and have not explored tuning down the induction of the fusion proteins. However, in future studies, using stable rather than transient transfection is advisable. Indeed, transient transfection may lead to heterogeneous populations of cells that exhibit a wide range of expression levels. In those cells where the fusion proteins are highly overexpressed interactomes may not faithfully reflect physiological interactions and complicate the interpretation of BioID data. Using stable cell lines for the Cdc25C/14-3-3ɛ pair led to lower expression of both fusion proteins but CBirA*-Cdc25C was still overexpressed. Rather than a strong discrepancy in the co-expression of both fusion proteins, we believe it probably reflects that Cdc25C is expressed at much lower levels than 14-3-3ɛ (ref. 58). Hence, if relative low expression of both fusion proteins is needed, one may turn to a dual plasmid system or a single plasmid in which co-expression is driven by two promoters of different strength. Alternatively, tagging through genome editing would ensure physiological expression levels. Of note, as we observed stronger re-activation with our split-BioID assay based on the E256/G257 splitting site than with the alternative E140/Q141 splitting site12, it will most probably be better suited when low expression levels of the fusion proteins are desired.
As we observed that the extent of biotinylation was depending on which protein was appended to the NBirA* or CBirA* fragment, we recommend testing both iterations when trying split-BioID on a pair of proteins. Similarly, N- and C-terminal fusion may be also tested if the proteins of interest can be tagged on both ends. The long flexible linkers we have used in our assay were taken from another PCA17 and worked for all the proteins we tested, hence they are a good starting point when testing the assay. To modulate the labelling range of the assay, one may envisage using shorter or longer linkers. However, as for any assay making use of fusion proteins, the length and nature of the linkers may need to be optimized for each protein pair studied.
To demonstrate the usefulness of the technique, we have applied split-BioID to the miRNA silencing pathway. Remarkably, activating the same protein (Ago2) with two different interacting partners (Dicer or TNRC6C) led to the labelling and identification of two very distinct sets of proteins reflecting two different functional steps in the life cycle of Ago2 and miRISC maturation. Importantly, as opposed to AP-MS, split-BioID was able to identify components of the major downstream effectors of Ago such as the CCR4/NOT deadenylation complex. As Ago2 is also reported to act at various defined cellular locations, such as the nucleus, the endoplasmic reticulum or multi-vesicular bodies59, we plan to exploit the power of split-BioID to analyse the elusive composition of the corresponding protein complexes.
Two other techniques have properties comparable to split-BioID. A conditional proteomics approach was recently used to specifically identify proteins involved in zinc homoeostasis60. However, it necessitated the chemical synthesis of a custom Zn2+-responsive labelling reagent, which makes the approach difficult to generalize. Moreover, as in AP-MS, the proteins identified are not assigned to specific complexes. A second approach, applied in yeast, proposes a non-radioactive pulse chase epitope labelling for the time-resolved AP of nascent protein complexes61. It relies on the incorporation of a non-natural amino acid and a riboswitch-controlled translation arrest. Whether this can be applied to another organism than yeast, notably for the identification of a suitable riboswitch and to obtain sufficient quantities of sample for MS analysis, remains to be addressed. In addition, as the bait must be purified at different time points following chase, the method can only be applied to monitor a relatively slow maturing complex and requires tightly timed handling to capture a specific maturation step. By contrast, split-BioID is activated in the native cellular environment only when two proteins interact, whenever the interaction takes place. With its ability to unbiasedly probe, in a single and simple assay, complexes building around a pair of interacting proteins, split-BioID is thus unique in the toolbox of methods for the analysis of PPI.
Altogether, we present a technique that will complement existing methods for the study of dynamics PPI networks and the assembly of protein complexes. split-BioID is a readily available method that only necessitates standard lab equipment and reagents. With the possibility to control the spatiotemporal activation of BirA*, we expect split-BioID will also be a very valuable tool for the otherwise challenging study of organelle contact sites or the protein composition of dynamic RNA granules, and hence contribute to the definition of high-resolution subcellular maps.
Plasmids and antibody
The plasmids, primers and antibodies used in this study are provided in the Supplementary Tables 1–5
Low passage HELA 11ht cells, a subclonal HeLa-CCL2 cell line, stably expressing the reverse tetracycline-controlled transcription activator rtTA-M2 and containing a locus for Flp-recombinase-mediated cassette exchange62 were obtained from Dr Kai Schönig (ZI Mannheim). Cells were regularly tested for mycoplasma contamination. Cells were grown in DMEM medium (Sigma) containing 10% tet-free bovine serum (Biowest), 200 μg ml−1 HygromycinB (Sigma) and 200 μg ml−1 G418 (Sigma).
Construction of plasmids
The different split-BioID constructs were designed for co-expression using the pSF3 backbone that harbours a bidirectional tetracycline-responsive promotor51. NBirA* and CBirA* fragments were amplified by PCR using a pSF3-BirA* plasmid as a template, and introducing either SalI and AscI or PacI and BglII as cutting sites, respectively. CBirA* fragment amplification was done using the C66, C271 or C274 forward primer together with the BirA* reverse primer. NBirA* fragment was amplified using the BirA* forward primer together with the N65, N270 or N273 reverse primer. FRB was amplified with additional restriction sites for FseI and PacI while FKBP was designed with AscI and BamHI. The glycine–serine linkers were either integrated between FRB and CBirA* using PacI (linker 1) or NBirA* and FKBP using AscI (linker 2) restriction sites.
The optimal split-BioID plasmids (four combinations of E256/G267) were also designed with the pSF3 backbone but CBirA* and NBirA* were ordered as gBlocks (IDT), fused to a sequence coding for the same glycine–serine linkers (QISYASRGGGSSGG and GGGSSGGQISYASRG) that were used in a split luciferase-based PCA17, and to specific restriction sites to insert the fusion proteins of interest. Most fusion proteins used were either integrated via PmeI and PacI restriction sites or ClaI and MluI depending on the fusion to the CBirA* part or the NBirA* part, respectively. Fusion proteins where these restriction sites could not be used were designed using the NEBuilder tool. All fusion proteins were amplified by PCR.
E140/Q141 construct was designed by replacement of the E256/G267 sBirA* parts in construct 2 with PCR amplified FKBP-NBirA*E140 and CBirA*Q141 using ClaI/BamHI and EcoRI/BglII restriction sites, respectively.
For expression of recombinant proteins, the CED domain of TNRC6C (amino acids 1369–1690) was amplified by PCR and transferred using the restriction sites BamHI and NheI to a modified pMal-c2x plasmid (New England Biolabs) that allows N-terminal tagging with MBP and C-terminal tagging with six histidines. The corresponding AAGL coding mutant was obtained by site-directed mutagenesis. The two fragments of GIGYF2 (amino acids 532–740 and 607–740) were amplified by PCR and transferred using the restriction sites MfeI and NotI into a pGEX-6p plasmid (GE Healthcare) that allows N-terminal tagging with GST.
For each sample, 35 μl slurry of protein-coupled magnetic beads (CST or NEB (25 μl slurry)) was used. Beads were coupled to 2.5–5 μg antibody at 4 °C for 1 h in wash buffer (50 mM Tris pH 7.4, 300 mM NaCl, 1 mM MgCl2, 0.5% NP-40). Cell lysates were prepared as described above (Screening for biotinylation). Same protein amounts (100–250 μg) were loaded on pre-coupled beads, the samples were then adjusted to 250 μl with lysis buffer, and incubated overnight at 4 °C on a rotating wheel. On the next day, the beads were washed four times with IP wash buffer and elution was done by either boiling (in case of anti-Myc IP) or incubation with FLAG peptide (150 ng μl−1 in TBS) for 30 min at 4 °C. The resulting samples were analysed by western blot.
For co-IP of endogenous proteins HeLa 11ht cells were grown in a 10 cm dish and collected in 1.4 ml lysis buffer, which was incubated for 30 min at 4 °C. Cleared lysates where treated with or without microccocal nuclease (14 gel units per μl, NEB) and 1.5 mM CaCl2 for 25 min at room temperature (RT). Samples were afterwards loaded on pre-coupled beads using either anti-Ago2, anti-TNRC6A, anti-GIGYF2 or anti IgG control antibodies (each 5 μg). Incubation, washing and elution were performed as described above.
Screening for biotinylation
HeLa 11ht cells were seeded at a concentration of 1 × 105 cells per well of a six-well plate or 8 × 105 cells per 10 cm dish the day before transfection. Transfection was performed using polyethylenimine (Polysciences, Inc.) in a 2:1 (w/w) ratio to the added DNA amount. Plasmid DNA (3 μg for six-well and 6 μg for 10 cm), polyethylenimine and DMEM (without serum) were mixed in a total volume of 500 μl, incubated for 5 min at RT and added to the cells. The media of the cells was changed directly before transfection. The day after transfection, biotin (Sigma) was added to the medium (50 μM) and the cells were induced with 200 ng ml−1 doxycycline (Sigma). In addition, for FRB/FKBP constructs, rapamycin (Invivogen) was added to 100 nM. Lysates were prepared 24 h after induction as follow: cells were washed once with PBS and scraped with 100 μl lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT and complete protease inhibitor (Roche)) followed by a centrifugation step at 14,000g for 10 min at 4 °C. Protein amounts were determined with a Bradford assay (Expedeon) and equal protein amounts were loaded on SDS–PAGE gels and analysed by western blot.
Western blot analysis
After SDS–PAGE, proteins were transferred to a low fluorescence PVDF membrane (Millipore) using the high molecular weight programme of the Trans-Blot Turbo Transfer System (BioRad) or overnight transfer (80 mA, 4 °C) in a Mini Trans-Blot cell (BioRad). Membranes were blocked for 1 h in 5% skimmed milk in PBS at RT and then incubated with the appropriate primary antibodies (Supplementary Table 4). After three washes in PBS-T, membranes were incubated with IRDye-coupled secondary antibodies. After two washes in PBS-T, and one wash with PBS, fluorescent signals were detected by scanning the membranes with an Odyssey CLx imaging system (LI-COR). Quantification was performed with the LI-COR Image Studio software according to the distributor’s instructions. Relevant parts of the resulting images were cropped, and linear adjustment of lightness and contrast levels were applied to the whole cropped areas to optimize visualization of the bands without compromising the information of the original picture. Full scans of the blots used to assemble the figures are shown on Supplementary Fig. 7.
Construction of stable cell lines
For the construction of stable cell lines (Cdc25C/14-3-3ɛ constructs for split-BioID, and BirA*-Ago2 and BirA*-control proteins for Ago2 and control BioID, the control proteins were Rab11a, Lamp1, TGN38, GRASP65, RHD4 and Sec61β), HeLa 11ht cells that harbour a stably integrated Hygromycin-TK cassette flanked by flippase recognition target (FRT) sites were used. The split-BioID constructs were designed with the pSF3 backbone51, which also harbours the same FRT sites and are therefore compatible with Flp-recombinase-mediated cassette exchange in HeLa 11ht cells. Cells were seeded at a concentration of 1 × 105 cells per well of a six-well plate on the day before transfection, which was done using Lipofectamine 3000 (LifeTechnologies) following the manufacturer’s protocol (cotransfection of BirA* construct and Flp coding plasmid pPGKFLPobpA (addgene 13793)). Cells were transferred to a 10 cm dish 24 h after transfection and 50 μM ganciclovir (Sigma) was added 72 h after transfection to start the selection procedure. Approximately one week later, colonies had formed and could be picked and propagated. Cells were in total treated for at least 3 weeks with ganciclovir.
For each split-BirA* condition, three biological replicates were performed and analysed via MS. In addition, BioID runs were performed with stable cell lines expressing six BirA*-tagged unrelated proteins (Sec61β, RHD4, GRASP65, TGN38, Lamp1 and Rab11a), these six runs were used as controls for the general background of the technique. Three to four 10 cm dishes per condition (Dicer/TNRC6C/Ago2 constructs) were transfected with the appropriate split-BioID constructs as described above. On the next day, the cells from each 10 cm dish were transferred to 15 cm dishes in medium containing 50 μM biotin, and 200 ng ml−1 doxycycline to induce production of the CBirA* and NBirA* fusion proteins. For stable cell lines, 8 × 15 cm (Cdc25/14-3-3ɛ constructs) or 2 × 15 cm dishes (BirA*-Ago2 and BirA*-controls) per condition were seeded with a cell amount of 2.6 × 106 cells per dish in biotin (50 μM)-containing medium, and directly induced with 200 ng ml−1 (Cdc25/14-3-3ɛ constructs) or 25 ng ml−1 doxycycline (BirA*-Ago2 and BirA*-controls). Twenty-four hour post induction, cells were washed twice with PBS, and then scraped in 1.5 ml PBS. Cells were then pelleted (1,200g, 5 min), snap frozen in liquid nitrogen and stored at −80 °C. For cell lysis, cell pellets were resuspended in 1 ml lysis buffer (50 mM Tris pH 7.4, 500 mM NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1 × complete protease inhibitor (Roche)) at RT and then mechanically disrupted by 10 passages through a 25 G needle followed by sonication (Bioruptor plus sonification device (Diagenode), four cycles at high intensity, 30 s per cycle).
After sonication, Triton X-100 concentration was adjusted to 2% and sodium chloride concentration to 150 mM. Lysates were then centrifuged at 4 °C 16,000g for 10 min. Ten per cent of each supernatant was kept as input material and the rest was incubated in equal amounts (3–3.5 mg per sample depending on the experiment) with 200 μl Dynabeads MyOne Streptavidin C1 (Invitrogen, catalogue number 65002) at 4 °C overnight on a rotating wheel. Magnetic beads were equilibrated for 10 min at RT in equilibration buffer (50 mM Tris pH 7–4, 150 mM NaCl, 0.05% Triton X-100, 1 mM DTT) prior use. All washing steps were performed at RT on a rotating wheel each for 8 min. Beads were washed with four different washing buffers each two times. Wash buffer 1 (2% SDS in water), wash buffer 2 (50 mM HEPES pH7.4, 1 mM EDTA, 500 mM NaCl, 1% Triton X-100, 0.1% Na-deoxycholate), wash buffer 3 (10 mM Tris pH 8, 250 mM LiCl, 1 mM EDTA, 0.5% NP-40, 0.5% Na-deoxycholate), wash buffer 4 (50 mM Tris pH 7.4, 50 mM NaCl, 0.1% NP-40). The biotinylated proteins were eluted from the beads by boiling them for 15 min at 98 °C in 30 μl elution buffer (10 mM Tris pH 7.4, 2% SDS, 5% β-mercaptoethanol, 2 mM Biotin). Beads were then immediately removed and the samples stored at −20 °C.
Sample preparation for MS
For MS analysis, eluted samples were run on 4–20% RunBlue SDS precast gels (Expedeon) until they migrated 2–3 cm into the gel. The whole lane was sliced after staining with colloidal Coomassie Brilliant Blue G250 (ref. 63), excluding the streptavidin band. The samples were sent for analysis to FingerPrints proteomics (University of Dundee, UK). There, the samples were processed to overnight (16 h) trypsin digestion (Modified Sequencing Grade, Roche). The peptides were extracted from the gel and dried in a SpeedVac (Thermo Scientific). The peptides were then resuspended in 50 μl 1% formic acid, centrifuged and transferred to HPLC vials.
Liquid chromatography/MS analysis
The samples were loaded (15 μl injection volume) on an Ultimate 3000 RSLCnano liquid chromatography system (Thermo Scientific, running dual column set-up) coupled to a LTQ OrbiTrap Velos Pro (Thermo Scientific). The peptides were initially trapped on an Acclaim PepMap 100 (C18, 100 μM × 2 cm) trap column, and then separated on an Acclaim PepMap RSLC C18 column (75 μM × 50 cm) followed by a transfer line (20 μM × 50 cm) attached to an easy-spray emitter (7 μM ID) (Thermo Scientific) to the MS via an easy-spray source with temperature set at 50 °C and a source voltage of 2.5 kV. Peptides were resolved in a gradient of acetonitrile in 0.08% formic acid, increasing the percentage of acetonitrile from 2 to 40% within 120 min, and to 98% within an addition 25 min.
Mass spectra were acquired in a data-dependent mode with automatic switching between MS and MS/MS scans using a top 15 method. Full MS scans were acquired in the Orbitrap mass analyzer over an m/z 350–1,800 range with a resolution of 60,000 and a target value of 106 ions. Peptide fragmentation was performed with the collision-induced dissociation mode. MS/MS spectra were acquired with a target value of 5,000 ions. Ion selection threshold was set to 5,000 counts.
MS data analysis
Raw MS files were analysed by MaxQuant64 version 18.104.22.168. MS/MS spectra were searched with the built-in Andromeda search engine against the Uniprot-human database (downloaded in March 2016) to which common contaminants and reverse sequences of all entries had been added. The search included variable modifications of methionine oxidation, N-terminal acetylation and lysine biotinylation, and fixed modification of carbamidomethyl cysteine. Minimal peptide length was set to seven amino acids and a maximum of two miscleavages was allowed. The false discovery rate (FDR) was set to 0.01 for peptide and protein identifications. For comparison between samples, we used LFQ65 with a minimum of two ratio counts to determine the normalized protein intensity. We activated the ‘match between run’ option.
The data were then processed using Perseus version 22.214.171.124 (ref. 66). Identifications from the reverse database, common contaminants and proteins only identified through a modification peptide were removed. Label-free intensities were then logarithmized (base 2) and the samples were then grouped according to the replicates with the six BioID runs on unrelated proteins defined as control group. At least two valid values across the three replicates were required for each identified protein. Following the Perseus analysis pipeline, empty values were imputed with random numbers from a normal distribution to simulate low abundance values below the detection limit of the instrument. For each split-BioID condition, a two-sample t-test based on permutation-based FDR statistics was then applied (250 permutations; FDR=0.07; S0=0.1) to identify specific hits over control. To further filter the data, we kept the resulting hits that had a median LFQ across the three biological replicates at least twice as high as GFP split-BioID sample.
Protein tables are given as Supplementary Data 1–3.
Knockdown experiments were performed in 96-well plates using previously described stable inducible HeLa cell lines co-expressing a firefly luciferase control reporter and either a renilla luciferase reporter appended to the 3′UTR of the let-7 miRNA target Hmga2 or a mutant thereof with disrupted miRNA-binding sites51. Transfection was performed with jetPrime (Polyplus Transfection) transfection reagent and 50 nM of GIGYF2 esiRNA (Sigma), 16.67 nM of each TNRC6A, B and C siRNA, 16.67 nM of each CNOT1, CNOT 7 and 8, or 50 nM of control siRNA (Qiagen, 1027281) following the manufacturer’s protocol. The siRNA targeting the TNRC6 proteins and CNOT subunits were previously described51. Twenty-four hour after transfection, media was changed and knockdown was analysed 48 h after transfection via western blot and luciferase assay.
Immunofluorescence of sBirA* constructs was performed with transient transfected cells detecting the FLAG- and Myc-tag and biotinylated proteins. Cells were fixed with 4% formaldehyde for 15 min at RT followed by three washing steps with PBS each 5 min. Permeabilization was performed with 0.2% Triton X-100 in PBS for 20 min at RT. Before incubation of primary antibodies over night at 4 °C, the cells were blocked in 5% BSA/2% goat serum/2% donkey serum in 0.2% Triton X-100/PBS for 1 h at RT. Next day, cells were washed 4 × with 0.2% Triton X-100/PBS for 5 min. Incubation with secondary antibodies was done for 2 h at RT followed by three washing steps in 0.2% Triton X-100/PBS and one with PBS each 5 min. DAPI staining was done for 20 min and cells were afterwards washed again two times with PBS prior fixation with Fluoromount-G (Southern Biotech). Antibodies and dilutions are listed in Supplementary Table 4. Pictures were taken with the × 100 objective of a Perkin Elmer ERS-6 spinning disk confocal microscope controlled by the Velocity software at the Heidelberg Nikon imaging centre. Minimum and maximum displayed values can be found in Supplementary Table 5. Cutoffs for transfected cells were set against untransfected HeLa 11ht cells for all IF pictures and additionally against cells which had not been treated with primary antibodies (not depicted).
Expression and purification of recombinant proteins
GST-tagged GIGYF2 fragments and MBP-His6-tagged TNRC6C fragments were expressed in BL21(DE3) cells (New England Biolabs). Cells were grown in 500 ml LB medium at 37 °C until OD600 reached 0.6. Thereafter, isopropyl β-D-1-thiogalactopyranoside was added to 0.1 mM to induce protein expression and the cells were grown overnight at 16 °C. The cells were then collected by centrifugation and resuspended in lysis buffer (for GST fusions: PBS+1 mM DTT, for MBP-His6 fusions: 50 mM Na2HPO4, pH=8, 500 mM NaCl, 20 mM imidazole, 0.5% Triton X-100, 1 mM DTT). Cells were lysed using a French press and processed for protein purification. GST fusions were purified on glutathione-coupled beads (glutathione sepharose 4B, GE Healthcare) following the vendor’s instructions (washing buffer, PBS, 1 mM DTT; elution buffer, 20 mM Tris pH 8, 150 mM NaCl, 20 mM glutathione, 1 mM DTT). MBP-His6 fusions were first purified on Ni-NTA-coupled beads (Ni sepharose high performance GE Healthcare) by incubation of the lysates for 1 h at 4 °C. Followed by two washing steps with wash buffer 1 (100 mM HEPES, pH=7.4, 500 mM NaCl, 50 mM imidazole, 0.5% Triton X-100) and one with wash step buffer 2 (100 mM HEPES, pH=7.4, 500 mM NaCl, 50 mM imidazole, 0.1% Triton X-100). Elution (100 mM HEPES, pH=7.4, 200 mM NaCl, 250 mM imidazole) was performed for 5 min at 4 °C. The eluted sample was then diluted in column buffer (50 mM Tris-HCl, pH=7.4, 200 mM NaCl, 1 mM EDTA, 1 mM DTT) and then incubated with 250 μl pre-equilibrated amylose-coupled beads (Amylose resin, New England Biolabs). The beads were washed three times with column buffer and elution performed 0.5 ml elution buffer (column buffer plus 10 mM maltose).
In vitro binding assay
MBP-CED (WT or AAGL)-His6 and GST-GIGYF2 fragments (600 pmol each) were mixed together in 100 μl binding buffer (50 mM HEPES, pH=7.4, 150 mM NaCl, 20 mM imidazole, 0.05% Triton X-100, 1 mM DTT) for 30 min at RT. A measure of 10 μl of Ni-NTA-coupled beads were then added to each reaction and incubation resumed for 30 min. The beads were then washed five times with 400 μl cold binding buffer. Bound material was then eluted with 50 μl binding buffer containing 250 mM imidazole.
Following doxycycline-mediated induction, cells were lysed using cytoplasmic lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA pH 8, 0.5% NP-40) for 20 min at 4 °C. FL and RL activities were measured from the lysates on a Xenius XL microplate luminometer (SAFAS Monaco) using the Dual-Luciferase Reporter Assay System (Promega).
GO enrichments analysis
For GO enrichment analysis, we used the VLAD application67 with the human proteome as background. VLAD uses the hypergeometric test for determining significance. To adjust P values, accounting for multiple testing, VLAD calculates a q value that is based on the concept of the positive false discovery rate.
STRING interaction networks analysis
On Fig. 9, data were tested for normality using the Shapiro–Wilk test. The null hypothesis for this test is normal data. Statistical significances were calculated on the normally distributed data using a two-tailed paired Student’s t-test. All the compared data had similar variance.
The MS proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository69 with the data set identifier PXD005005. The data that support the findings of this study are available from the corresponding author on request.
How to cite this article: Schopp, I. M. et al. Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nat. Commun. 8, 15690 doi: 10.1038/ncomms15690 (2017).
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Jonas, S. & Izaurralde, E. Towards a molecular understanding of microRNA-mediated gene silencing. Nat. Rev. Genet. 16, 421–433 (2015).
Frohn, A. et al. Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells. Mol. Cell Proteomics 11, 1442–1456 (2012).
Landthaler, M. et al. Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. RNA 14, 2580–2596 (2008).
Li, S. et al. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc. Natl Acad. Sci. USA 111, 6970–6975 (2014).
Braun, J. E., Huntzinger, E., Fauser, M. & Izaurralde, E. GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Mol. Cell 44, 120–133 (2011).
Chekulaeva, M. et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat. Struct. Mol. Biol. 18, 1218–1226 (2011).
Fabian, M. R. et al. miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT. Nat. Struct. Mol. Biol. 18, 1211–1217 (2011).
Roux, K. J., Kim, D. I., Raida, M. & Burke, B. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 196, 801–810 (2012).
Kwon, K. & Beckett, D. Function of a conserved sequence motif in biotin holoenzyme synthetases. Protein Sci. 9, 1530–1539 (2000).
Kim, D. I. et al. Probing nuclear pore complex architecture with proximity-dependent biotinylation. Proc. Natl Acad. Sci. USA 111, E2453–E2461 (2014).
Lambert, J. P., Tucholska, M., Go, C., Knight, J. D. & Gingras, A. C. Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. J. Proteomics 118, 81–94 (2015).
De Munter, S. et al. Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions. FEBS Lett. 591, 415–424 (2017).
Banaszynski, L. A., Liu, C. W. & Wandless, T. J. Characterization of the FKBP.rapamycin.FRB ternary complex. J. Am. Chem. Soc. 127, 4715–4721 (2005).
Wilson, K. P., Shewchuk, L. M., Brennan, R. G., Otsuka, A. J. & Matthews, B. W. Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains. Proc. Natl Acad. Sci. USA 89, 9257–9261 (1992).
Chapman-Smith, A., Mulhern, T. D., Whelan, F., Cronan, J. E. Jr & Wallace, J. C. The C-terminal domain of biotin protein ligase from E. coli is required for catalytic activity. Protein Sci. 10, 2608–2617 (2001).
Chakravartty, V. & Cronan, J. E. The wing of a winged helix-turn-helix transcription factor organizes the active site of BirA, a bifunctional repressor/ligase. J. Biol. Chem. 288, 36029–36039 (2013).
Luker, K. E. et al. Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals. Proc. Natl Acad. Sci. USA 101, 12288–12293 (2004).
Hutchins, J. R. & Clarke, P. R. Many fingers on the mitotic trigger: post-translational regulation of the Cdc25C phosphatase. Cell Cycle 3, 41–45 (2004).
Morris, M. C., Heitz, A., Mery, J., Heitz, F. & Divita, G. An essential phosphorylation-site domain of human cdc25C interacts with both 14-3-3 and cyclins. J. Biol. Chem. 275, 28849–28857 (2000).
Xu, X. et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat. Commun. 8, 14059 (2017).
Bonnet, J., Coopman, P. & Morris, M. C. Characterization of centrosomal localization and dynamics of Cdc25C phosphatase in mitosis. Cell Cycle 7, 1991–1998 (2008).
Tahbaz, N., Carmichael, J. B. & Hobman, T. C. GERp95 belongs to a family of signal-transducing proteins and requires Hsp90 activity for stability and Golgi localization. J. Biol. Chem. 276, 43294–43299 (2001).
MacRae, I. J., Ma, E., Zhou, M., Robinson, C. V. & Doudna, J. A. In vitro reconstitution of the human RISC-loading complex. Proc. Natl Acad. Sci. USA 105, 512–517 (2008).
Nakanishi, K. Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins? Wiley Interdiscip. Rev. RNA 7, 637–660 (2016).
Leung, A. K., Calabrese, J. M. & Sharp, P. A. Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Proc. Natl Acad. Sci. USA 103, 18125–18130 (2006).
Liu, J., Valencia-Sanchez, M. A., Hannon, G. J. & Parker, R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell. Biol. 7, 719–723 (2005).
Mauri, M. et al. Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution. Nucleic Acids Res. 45, 938–950 (2017).
Chendrimada, T. P. et al. TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature 436, 740–744 (2005).
Haase, A. D. et al. TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing. EMBO Rep. 6, 961–967 (2005).
Srivastava, M. et al. Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis. Nat. Commun. 6, 6253 (2015).
Muddashetty, R. S. et al. Reversible inhibition of PSD-95 mRNA translation by miR-125a, FMRP phosphorylation, and mGluR signaling. Mol. Cell 42, 673–688 (2011).
McCann, C. et al. The Ataxin-2 protein is required for microRNA function and synapse-specific long-term olfactory habituation. Proc. Natl Acad. Sci. USA 108, E655–E662 (2011).
Nishi, K., Nishi, A., Nagasawa, T. & Ui-Tei, K. Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19, 17–35 (2013).
Schraivogel, D. et al. Importin-beta facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. 43, 7447–7461 (2015).
Ozgur, S., Chekulaeva, M. & Stoecklin, G. Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies. Mol. Cell Biol. 30, 4308–4323 (2010).
Mathys, H. et al. Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression. Mol. Cell 54, 751–765 (2014).
Iki, T., Yoshikawa, M., Meshi, T. & Ishikawa, M. Cyclophilin 40 facilitates HSP90-mediated RISC assembly in plants. EMBO J. 31, 267–278 (2012).
Martinez, N. J., Chang, H. M., Borrajo Jde, R. & Gregory, R. I. The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly. RNA 19, 1583–1593 (2013).
Pare, J. M., LaPointe, P. & Hobman, T. C. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol. Biol. Cell 24, 2303–2310 (2013).
Woehrer, S. L. et al. A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms. EMBO J. 34, 559–577 (2015).
Iki, T. et al. In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90. Mol. Cell 39, 282–291 (2010).
Iwasaki, S. et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol. Cell 39, 292–299 (2010).
Echeverria, P. C. & Picard, D. Molecular chaperones, essential partners of steroid hormone receptors for activity and mobility. Biochim. Biophys. Acta 1803, 641–649 (2010).
Iwasaki, S. et al. Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex. Nature 521, 533–536 (2015).
Kakihara, Y. & Houry, W. A. The R2TP complex: discovery and functions. Biochim. Biophys. Acta 1823, 101–107 (2012).
Lee, Y. et al. The role of PACT in the RNA silencing pathway. EMBO J. 25, 522–532 (2006).
Morita, M. et al. A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development. Mol. Cell Biol. 32, 3585–3593 (2012).
Rudel, S., Flatley, A., Weinmann, L., Kremmer, E. & Meister, G. A multifunctional human Argonaute2-specific monoclonal antibody. RNA 14, 1244–1253 (2008).
Ash, M. R. et al. Conserved beta-hairpin recognition by the GYF domains of Smy2 and GIGYF2 in mRNA surveillance and vesicular transport complexes. Structure 18, 944–954 (2010).
Bazzini, A. A., Lee, M. T. & Giraldez, A. J. Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish. Science 336, 233–237 (2012).
Béthune, J., Artus-Revel, C. G. & Filipowicz, W. Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells. EMBO Rep. 13, 716–723 (2012).
Djuranovic, S., Nahvi, A. & Green, R. miRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science 336, 237–240 (2012).
Mishima, Y. et al. Translational inhibition by deadenylation-independent mechanisms is central to microRNA-mediated silencing in zebrafish. Proc. Natl Acad. Sci. USA 109, 1104–1109 (2012).
Braun, J. E., Huntzinger, E. & Izaurralde, E. A molecular link between miRISCs and deadenylases provides new insight into the mechanism of gene silencing by microRNAs. Cold Spring Harb. Perspect. Biol. 4, a012328 (2012).
Andersen, J. S. et al. Proteomic characterization of the human centrosome by protein correlation profiling. Nature 426, 570–574 (2003).
Havugimana, P. C. et al. A census of human soluble protein complexes. Cell 150, 1068–1081 (2012).
Lam, S. S. et al. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat. Methods 12, 51–54 (2015).
Nagaraj, N. et al. Deep proteome and transcriptome mapping of a human cancer cell line. Mol. Syst. Biol. 7, 548 (2011).
Kim, Y. J., Maizel, A. & Chen, X. Traffic into silence: endomembranes and post-transcriptional RNA silencing. EMBO J. 33, 968–980 (2014).
Miki, T. et al. A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13, 931–937 (2016).
Stelter, P. et al. Monitoring spatiotemporal biogenesis of macromolecular assemblies by pulse-chase epitope labeling. Mol. Cell 47, 788–796 (2012).
Weidenfeld, I. et al. Inducible expression of coding and inhibitory RNAs from retargetable genomic loci. Nucleic Acids Res. 37, e50 (2009).
Dyballa, N. & Metzger, S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J. Vis. Exp. 30, e1431 (2009).
Cox, J. & Mann, M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat. Biotechnol. 26, 1367–1372 (2008).
Cox, J. et al. Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. Mol. Cell Proteomics 13, 2513–2526 (2014).
Tyanova, S. et al. The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat. Methods 13, 731–740 (2016).
Richardson, J. E. & Bult, C. J. Visual annotation display (VLAD): a tool for finding functional themes in lists of genes. Mamm. Genome 26, 567–573 (2015).
Szklarczyk, D. et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res. 43, D447–D452 (2015).
Vizcaino, J. A. et al. The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 41, D1063–D1069 (2013).
We thank Petra Hubbe for excellent technical support. We thank M. Chekulaeva (Berlin), G. Meister (Regensburg), D. Grimm (Heidelberg), S. Winkler (Nottingham), C. Freund (Berlin) and G. Stoecklin (Mannheim) for providing us with plasmids (see Supplementary Data), and K. Schönig (Mannheim) for the HeLa 11ht cell line. We thank the Ruiz de Almodóvar group (Heidelberg), especially P. Himmels for the help and support with the immunofluorescence and sharing antibodies. We thank F. Wieland, H. Hillen and J. Schopp for comments on the manuscript, Life Science Editors for editing assistance. This work was supported by the excellence initiative of the German research council (DFG-EXC81), I.M.S. and J.B. were partially supported by a collaborative research grant (DFG-SFB638) of the German research council.
The authors declare no competing financial interests.
Supplementary Figures, Supplementary Tables and Supplementary Method (PDF 13526 kb)
Proteomics dataset for Cdc25C/14-3-3e split BioID (XLSX 199 kb)
Proteomics dataset for TNRC6C/Ago2 and Dicer/Ago2 split BioID (XLSX 280 kb)
Proteomics dataset for Ago2 BioID (XLSX 246 kb)
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Schopp, I., Amaya Ramirez, C., Debeljak, J. et al. Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nat Commun 8, 15690 (2017). https://doi.org/10.1038/ncomms15690
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