Table 1 Two-phase fermentation performances of the engineered E. coli strains under various conditions.

From: Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli

Strain Condition TPA (gl1) Byproduct* p TA (gl1) Overall productivity (gl1h1) Maximum productivity (gl1h1) Yield (mol%)
DH5α/Piii 10; 5; 10§; Manual; 1.4|| 2.743 1.688 0.043 0.226 57.1
DH5α/Piii 20; 5; 10§; Manual; 1.4|| 1.240 2.185 0.020 0.113 31.7
DH5α/Piii 10; 30; 40§; DO-stat; 1.4|| 6.662 0.184 0.278 0.748 96.7
DH5α/Piii 20; 30; 40§; DO-stat; 1.4|| 5.844 6.007 0.126 0.861 44.4
DH5α/Piii 20; 30; 40§; DO-stat; 0.4|| 6.905 1.902 0.150 0.546 74.8
  1. OA, oleyl alcohol; pTA, p-toluic acid; pX, p-xylene; TPA, terephthalic acid.
  2. *Only pTA was detected as the byproduct without other pathway intermediates.
  3. The amount of pX (g) that was fed into the fermenter.
  4. The OD600 value at which point cell culture was induced.
  5. §The OD600 value at which point pX feeding was started.
  6. ||The feeding rate of the OA containing dissolved pX (ml min−1).