(a) Time lapse fluorescence images of a single DNA molecule. The blue circles show the 2D positions of the molecule determined by the tracking. Scale bar=1 μm. (b) Diffusion trajectory of ColE1 DNA mapped onto the latticed 2D space with 0.16 μm side length of the lattice (See Supplementary Note 1). (c) MSD-Δt profile of ColE1 DNA (Δt=6.4 ms). Rg shows the radius of gyration of ColE1 DNA. The red and blue circles highlight the parts of the MSD-Δt profile that show sub-diffusive and linear-diffusive regimes. The green line is the theoretical MSD-Δt profile. (d) Temporal P25 profile of ColE1 DNA. μp shows the mean P25 value.