Figure 7: Time-concentration curves of XDT and DT as well as Taxol in the reactions and the Taxol yields in the one-pot reaction system. | Nature Communications

Figure 7: Time-concentration curves of XDT and DT as well as Taxol in the reactions and the Taxol yields in the one-pot reaction system.

From: Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production

Figure 7

(a) Time–concentration curves of Taxol with DT as the substrate and catalysed by different concentrations of DBATG38R/F301V. (b) Time–concentration curves of DT with XDT as the substrate and catalysed by different concentrations of LXYL-P1-2. (c) Time–concentration curves of DT and Taxol catalysed by 1.5 mg ml−1 of DBATG38R/F301V without DBATG38R/F301V supplement. (d) Time-concentration curves of DT and Taxol catalysed by 1.5 mg ml−1 of DBATG38R/F301V with DBATG38R/F301V supplement (downward black triangle, adding time). (e) Time–concentration curves of XDT, DT and Taxol in the one-pot reaction system (0.5 mg ml−1 of LXYL-P1-2 plus 1.5 mg ml−1 of DBATG38R/F301V) without DBATG38R/F301V supplement. (f) Time–concentration curves of XDT, DT and Taxol in the one-pot reaction system with DBATG38R/F301V supplement (downward black triangle, adding time). (g) Scaling-up of the one-pot reaction. (h) HPLC analysis of the one-pot reaction at 15 h (reaction volume: 50 ml). The data represent the means±s.d., n=3.

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