Figure 5 : Schematic overview of the non-chromatographic purification of TM peptides.

From: Efficient protein production inspired by how spiders make silk

Figure 5

The hydrophobic nature of surfactant peptides rSP-C33Leu and rKL4 allows for purification through salt precipitation and ethanol extraction. The figure describes each step in the purification protocol and further explains the denotations P1, S1, P2, S2 and T in Fig. 4e,f.