Malignant pleural effusion (MPE) is the lethal consequence of various human cancers metastatic to the pleural cavity. However, the mechanisms responsible for the development of MPE are still obscure. Here we show that mutant KRAS is important for MPE induction in mice. Pleural disseminated, mutant KRAS bearing tumour cells upregulate and systemically release chemokine ligand 2 (CCL2) into the bloodstream to mobilize myeloid cells from the host bone marrow to the pleural space via the spleen. These cells promote MPE formation, as indicated by splenectomy and splenocyte restoration experiments. In addition, KRAS mutations are frequently detected in human MPE and cell lines isolated thereof, but are often lost during automated analyses, as indicated by manual versus automated examination of Sanger sequencing traces. Finally, the novel KRAS inhibitor deltarasin and a monoclonal antibody directed against CCL2 are equally effective against an experimental mouse model of MPE, a result that holds promise for future efficient therapies against the human condition.
The pleural cavities of two million cancer patients per year are affected by malignant pleural effusion (MPE), caused by primary malignant pleural mesothelioma or by metastatic cancers originating from the lung, breast, gastrointestinal tract or elsewhere1. MPE manifests with vascular leakiness that leads to fluid accumulation in the pleural space and is etiologically associated with fulminant inflammation and neovascularization, rather than mere tumour-induced lymphatic obstruction2. However, the reason why some patients with pleural tumours develop MPE while others do not remains unknown3. This dichotomous phenotype of ‘wet’ pleural carcinomatosis associated with a MPE versus ‘dry’ pleural carcinomatosis without a MPE is critical, since patients with even minimal effusions face a worse prognosis and limited treatment options3,4. Our previous work on experimental mouse models of MPE revealed that pleural tumour-secreted C–C motif chemokine ligand 2 (CCL2) mediates MPE formation by stimulating angiogenesis and vascular leakage and by driving myeloid cells, including monocytes and mast cells, from the bone marrow to the pleural metastatic milieu5,6,7. However, the molecular culprits responsible for tumour cell CCL2 secretion and subsequent MPE precipitation remain unknown.
EGFR, KRAS, PIK3CA, BRAF, MET, EML4/ALK, RET and other mutations have been identified in pleural tumour biopsies and pleural fluid aspirates from MPE patients8,9,10,11,12,13,14,15,16. EGFR mutations were recently implicated in MPE development and patients with KRAS-mutant lung adenocarcinomas were found to have more frequent pleural metastases compared with wild-type ones17,18,19. However, no study has addressed the role of KRAS mutations in MPE development.
We hypothesized that the ability of a tumour cell to induce a MPE once it homes to the pleural space is linked with an underlying molecular signature. To test this and to model the biologic events that follow pleural metastasis, we determined the mutation status of multiple murine and human cancer cell lines and simultaneously tested their ability to induce MPE by directly injecting them into the pleural space of appropriate recipient mice. Our results indicate that pleural homed cancer cells harboring activating KRAS mutations are competent of MPE induction. Moreover, we provide evidence that this genotype-phenotype link is primarily mediated via mutant KRAS-dependent CCL2 signalling that results in the recruitment of CD11b+Gr1+ myeloid cells to the pleural space, a phenomenon requiring intact splenic function. Importantly, we show that KRAS mutations are detectable in human MPE by careful analyses of Sanger sequencing traces and that mutant KRAS-mediated MPE is actionable.
A link between KRAS mutations and MPE
To identify a possible MPE-associated genotype, we cross-examined five murine C57BL/6-derived and five human cancer cell lines for genotype and MPE competence. For this, we directly injected 1.5 × 105 mouse or 106 human tumour cells or 3 × 106 HEK293T benign human embryonic kidney cells into the pleural cavities of C57BL/6 (mouse cells) or NOD/SCID (human cells) mice. In parallel, we Sanqer-sequenced the Kras, Egfr, Pik3ca and Braf transcripts of mouse cells after reverse-transcribing them to cDNAs and amplifying them with specific primers (Supplementary Table 1), and obtained mutation data for KRAS, EGFR, PIK3CA and BRAF genes of human cells from COSMIC20. KRAS mutations of human cells were also verified in-house. Among mouse cells, three Kras-mutant (Lewis lung carcinoma, LLC; MC38 colon adenocarcinoma; and AE17 malignant pleural mesothelioma, bearing heterozygous KrasG12C, KrasG13R, and KrasG12C mutations, respectively) and two Kras wild-type (B16F10 skin melanoma and PANO2 pancreatic adenocarcinoma) cell lines were identified, which were all free of additional mutations in Egfr, Pik3ca or Braf genes (Fig. 1a; Table 1). Among human cells, A549 lung adenocarcinoma cells and their derivatives, long-term passaged (LTP) A549 cells that have suffered Y chromosome loss, featured a heterozygous KRASG12S mutation, while SKMEL2 skin melanoma, HT-29 colon adenocarcinoma, and HEK293T human embryonic kidney cells were KRAS wild-type (Table 1). These human cell lines also had wild-type EGFR, PIK3CA and BRAF genes, with the exception of HT-29 cells that harbor BRAF and PIK3CA mutations20. KRAS-mutant cell lines exhibited enhanced KRAS mRNA expression and RAS activity compared to KRAS wild-type cells (Supplementary Fig. 1a–d). Interestingly, upon pleural injection to appropriate hosts, all cell lines produced extensive pleural carcinomatosis, but exclusively KRAS-mutant cells gave rise to MPE (Fig. 1b–d; Table 1). To definitely test this in an isogenic cellular system, we derived lung adenocarcinoma cell lines from C57BL/6 and FVB mice. For this, C57BL/6 mice received ten and FVB mice four weekly intraperitoneal injections of the lung carcinogen urethane (1 g kg−1), as described elsewhere21,22, and were killed after 10 months, followed by long-term lung tumour culture in vitro23. The resulting cell lines (C57BL/6 and FVB-derived urethane-induced lung adenocarcinoma, CULA and FULA cells, respectively) were tumourigenic when implanted subcutaneously in syngeneic mice. Importantly, three different FULA cell lines had three different Kras mutations (including Q61H, Q61R and G12V mutations), while CULA cells were Kras wild-type (Fig. 1a; Table 1). In accordance with the results from existing cell lines, all Kras-mutant FULA cell lines were MPE-competent, while Kras-wild-type CULA cells were not (Fig. 1b–d; Table 1). In summary, out of the 12 different cell lines tested, six out of six KRAS-mutant cell lines were MPE-competent and none out of six MPE-incompetent, while none out of six KRAS-wild-type cell lines was MPE-competent, and six out of six MPE-incompetent (P=0.0022; χ2-test), indicating a statistically significant association between mutant KRAS and MPE.
Myeloid cells in mutant KRAS-dependent MPE
Kras-mutant tumour cell-triggered MPE was clinically important as mice with MPE succumbed significantly (P<0.0001; log-rank test) earlier compared with mice with dry pleural carcinomatosis from Kras-wild-type cells (Supplementary Fig. 1e). In addition to early lethality, mutant Kras-dependent MPE development was associated with a massive influx of myeloid cells into the pleural space. This was investigated using irradiated C57BL/6 chimeras reconstituted with luminescent bone marrow from ubiquitously luminescent CAG.Luc.eGFP donor mice fully backcrossed to the C57BL/6 strain6,7,24. Fourteen days after pleural tumour cell injection, only chimeras injected with Kras-mutant tumour cells showed an increased thoracic bioluminescent signal (Supplementary Fig. 1f). This KRAS-dependent inflammatory response associated with MPE formation was predominated by both polymorphonuclear and mononuclear myeloid cells that expressed both CD11b and Gr1, and either Ly6c or Ly6g (Fig. 1e,f; Supplementary Figs 1g and 2a). MPE development triggered by KRAS-mutant cancer cells was associated with an influx of increased numbers of all kinds of myeloid cells into the pleural space, but not with the presence of newly-appearing morphologically or molecularly distinct cell types, since differential pleural cell counts and flow cytometry results were similar percentage-wise in mice with or without a MPE (Supplementary Fig. 2a). In addition to triggering a myeloid inflammatory response, KRAS-mutant pleural tumours and MPEs showed enhanced angiogenic and vasoactive potential in several in vivo assay systems compared with KRAS-wild-type tumours (Supplementary Fig. 2b–d). However, we did not detect an increased proliferative or clonogenic capacity specifically characterizing KRAS-mutant cells and tumours compared with KRAS-wild-type ones (Supplementary Fig. 2e–i). This was in accord with the equal total mass of pleural tumors per mouse observed across pleural-injected KRAS-mutant and wild-type tumour cells (Fig. 1b). Collectively, these results suggested that mutant KRAS-driven MPE is associated with induction of an inflammatory, angiogenic, and vasoactive response in the pleural space, but not necessarily with enhanced pleural tumour growth.
Mutant KRAS promotes MPE
To corroborate the link between KRAS mutations and MPE, we undertook both shRNA-mediated KRAS silencing in cell lines harboring mutant KRAS, as well as mutant KRAS overexpression in cell lines harboring wild-type KRAS. Stable transduction of six different Kras-mutant mouse tumour cells with lentiviral-delivered Kras-specific shRNA (shKras) resulted in diminished expression of both murine Kras isoforms (2A and 2B) and decreased RAS signalling compared with random (control, shC) shRNA, whereas overexpression of mutant KrasG12C isoforms in murine and human cell lines carrying wild-type KRAS via retroviral transduction enhanced the respective KRAS protein levels and increased RAS signalling (Supplementary Fig. 3). Manipulation of KRAS signalling did not result in obvious enhancements of tumour cell proliferation or survival in vitro; on the contrary, overexpression of KrasG12C2A in PANO2 cells and of KrasG12C2B in B16F10 cells slowed their growth rate (Supplementary Fig. 4). However, upon direct inoculation of all parental and daughter KRAS-modulated cell lines into the pleural space of appropriate (C57BL/6, FVB or NOD/SCID) host mice, all mice developed similar extent of pleural carcinomatosis, but expression of mutant KRAS was a cardinal determinant of MPE in all cell lines examined (Fig. 2a,b; Table 2; Supplementary Fig. 5). More specifically, Kras silencing universally abrogated MPE formation by LLC, MC38, AE17 and three different FULA cell lines, that is, in cells harbouring either KRAS G12C (LLC & AE17 cells), G13R (MC38 cells), G12V, Q61H or Q61R (FULA cells) mutations, whereas oncogenic KrasG12C expression conferred MPE competence to B16F10, PANO2, SKMEL2, and HEK293T cells. Remarkably: (i) expression of KrasG12C isoform 2A conferred enhanced MPE competence to PANO2 cells compared with KrasG12C isoform 2B, although the later was more abundantly expressed by KRAS-mutant cancer cells; and (ii) mutant KRAS expression converted even benign HEK293T cells to MPE competence (Fig. 2a,b; Table 2; Supplementary Fig. 5). Using pleural injection of parental and KRAS-modulated MC38 and PANO2 cells into C57BL/6 chimeras reconstituted with CAG.Luc.eGFP bone marrow, we identified that mutant KRAS is not only responsible for MPE development, but also for the associated pleural influx of CD11b+Gr1+ cells (Fig. 2c; Supplementary Fig. 5c,d). Taken together, these results indicated that mutant KRAS is dispensable for pleural tumour growth, but important for MPE development and for the associated systemic recruitment of CD11b+Gr1+ myeloid cells, and suggested that KRAS must be responsible for the secretion of a solute mediator of MPE by tumour cells.
KRAS-mutant tumour cells signal via CCL2 to host cells
To identify the MPE mediator(s) downstream of mutant KRAS and to tease out the transcriptional signature of mutant KRAS on tumour cells, we performed comparative microarray-based transcriptome profiling of Kras-mutant and wild-type mouse tumour cells versus benign airway cells. Unsupervised clustering according to global gene expression revealed that Kras-mutant cell lines clustered closely together (Supplementary Fig. 6a). Individual gene analysis identified 25 transcripts overrepresented more than 10-fold in KRAS-mutant, but not in KRAS-wild-type, cell lines compared with benign cells (Fig. 3a; Table 3). Microarray results were verified by qPCR and ELISA (Fig. 3b; Supplementary Figs 6b,c). Furthermore: (i) manipulation of mutant KRAS expression resulted in parallel changes in Ccl2 expression; (ii) cell culture media conditioned by KRAS-mutant tumour cells featured markedly elevated CCL2 levels compared with media conditioned by KRAS-wild-type tumour cells; and (iii) mice bearing in their pleural space KRAS-mutant tumour cells featured markedly elevated serum CCL2 levels compared with mice harbouring KRAS-wild-type tumour cells (Supplementary Fig. 6d–g). To corroborate CCL2 as the downstream effector of mutant KRAS that mediates MPE in vivo, we directly delivered LLC (KrasG12C), MC38 (KrasG13R), and PANO2 cells overexpressing KrasG12C isoform 2A into the pleural space of Ccr2-gene-deficient mice (Ccr2−/−; the gene encoding the cognate receptor of CCL2)25 and C57BL/6 controls. In accord with our hypothesis, Ccr2−/− mice were protected against MPE induced by all three Kras-mutant tumour cell lines and displayed reduced CCR2 expression by pleural fluid cells and decreased accumulation of CD11b+Gr1+ cells in the pleural space (Fig. 3c; Supplementary Fig. 6h,i). Collectively, these data suggest that mutant KRAS drives MPE development via systemic CCL2 signalling to CCR2+ host cells.
Mutant KRAS recruits splenic CD11b+Gr1+ cells to MPE
We next sought to identify the systemic recruitment patterns of myeloid cells during MPE development. For this, C57BL/6 chimeras reconstituted with CAG.Luc.eGFP bone marrow were inoculated with Kras-mutant pleural tumour cells and were serially imaged for bioluminescence. Although immediately after pleural tumour cell delivery the myeloid-emitted bioluminescent signal was primarily identified over the hematopoietic bones, it sequentially translocated to the upper left abdomen (days 10–12 post-tumour cell injection) before appearing in the thorax at days 12–14 post tumour cell injection (Supplementary Fig. 7a). Splenectomy abolished this abdominal myeloid-borne signal that was recapitulated from explanted spleens (Fig. 3d). In addition, CCR2+CD68+ myeloid cells were identified in the splenic marginal zones and the pleural cavities of mice with MPE induced by KRAS-mutant cells, but not of naive mice (Supplementary Fig. 7b). These results suggested that CD11b+Gr1+ myeloid cells are mobilized by mutant KRAS-driven CCL2-mediated signalling from the bone marrow to MPE via the spleen. On the basis of this evidence and the existing literature26,27,28, we hypothesized that the splenic passage of CD11b+Gr1+ cells is essential for MPE formation. To test this, we delivered MC38 cells (KrasG13R) or PANO2 cells expressing mutant KrasG12C isoform 2A or 2B to the pleural cavities of splenectomized and sham-operated C57BL/6 mice after allowing two weeks for recovery. Indeed, splenectomy markedly protected C57BL/6 mice from MPE, prolonged their survival, and prevented pleural accumulation of CD11b+Gr1+ myeloid cells (Fig. 3e; Supplementary Fig. 7c,d). Similarly, splenectomy protected NOD/SCID mice from A549-induced MPE (KRASG12S; Supplementary Fig. 7e), further suggesting that myeloid and not lymphoid splenic cells promote MPE in these lymphoid-deficient mice. Splenectomy-conferred protection was long-lived, as even mice collected 30 days post-tumour cell injection did not have MPE (Supplementary Fig. 7c). To address whether splenic CD11b+Gr1+ cells are required for MPE development, tumour-naive and tumour-bearing CAG.Luc.eGFP mice were used as splenocyte donors to splenectomized pleural MC38 (KrasG13R)-bearing C57BL/6 mice. These CAG.Luc.eGFP donors received pleural injections of saline (naive splenocyte), control shRNA-expressing MC38 cells (MC38 shC-educated splenocyte) or Kras specific-shRNA expressing MC38 cells (MC38 shKras-educated splenocyte) and 13 days later, their spleens were collected and processed to single-cell suspensions. In parallel, splenectomized or sham-operated C57BL/6 hosts received pleural MC38 cell injections. At post-injection day 9, splenectomized animals received five million intravenous splenocytes obtained from naive, shC or shKras MC38-bearing donors whereas, at post-injection day 13, these mice were analysed for MPE incidence, volume, survival, and for Luc+CD11b+Gr1+ recruited pleural cells (derived from transplanted splenocytes). Interestingly, only splenocytes from donors inoculated with MC38 cells bearing intact mutant KRAS signalling were able to translocate to the pleura and promote MPE formation in splenectomized mice harbouring pleural MC38 cells (Supplementary Fig. 7f–h). Taken together, these results indicated that KRAS-mutant pleural tumours induce the sequential recruitment of CD11b+Gr1+ cells from the bone marrow to the spleen and into the pleural cavity. Furthermore, that during MPE formation, bone marrow-borne, splenic CD11b+Gr1+ cells are conditioned by solute mediators secreted by KRAS-mutant pleural tumours (possibly CCL2) and functionally contribute to MPE development.
KRAS mutations in human MPE
We next Sanger-sequenced the KRAS transcripts of 12 human MPEs caused by metastatic lung adenocarcinomas according to established protocols16. Interestingly, KRAS mutations were present in numerous MPEs, but were not always readily detectable by automated Sanger sequencing trace analysis using BioEdit software29, since mutant base traces were often hidden underneath wild-type traces superimposed by the other KRAS allele, or by tumour-infiltrating stromal cells (Fig. 4a,b; Table 4). We also analysed recently published data of the site of recurrence of 481 resected non-small cell lung cancers according to KRAS and EGFR mutation status, and found that KRAS mutations overall were highly significantly (P<0.0001; Fischer’s exact test) associated with pleural recurrence (Table 4). We went on to derive cell lines from eight patients with lung adenocarcinoma-induced MPE that were initially tested KRAS wild-type. Interestingly, KRAS mutations were frequently identified in MPE cell lines initially tested wild-type (Fig. 4c–e; Table 4). These results suggested that: (i) KRAS mutations are present in a substantial proportion of patients with lung adenocarcinoma-caused MPE in Europe; (ii) KRAS mutation frequency may be underestimated in MPE samples analysed automatically; and (iii) our observations in mice may also hold true in humans.
Targeting KRAS is effective against MPE development
To determine the potential efficacy of KRAS inhibition against MPE, the novel KRAS inhibitor deltarasin30 was administered daily intraperitoneally at 15 mg kg−1, side-by-side with a saline control treatment, to mice with established pleural tumours. For this, treatments commenced at day 4–14 post-mouse tumour cell injections and at day 14 post-human tumour cell injections to allow initial tumour implantation in the pleural space6,7. At day 13 after pleural injection of MC38 cells (KrasG13R), deltarasin-treated C57BL/6 mice developed fewer and smaller MPEs, retarded pleural tumour dissemination and decreased pleural CD11b+Gr1+ accumulation compared with controls (Fig. 5a; Table 5). Furthermore, in vitro treatment of MC38 cells with deltarasin resulted in almost complete elimination of CCL2 secretion (Fig. 5b). To test the impact of KRAS blockade in a more human-relevant setting, NOD/SCID mice received deltarasin and control treatments starting at two weeks after pleural delivery of one million A549 cells (KRASG12S). At day 30 after tumour cell injection, deltarasin-treated mice had markedly decreased MPE volume and incidence compared with controls (Fig. 5c; Table 5). We also explored direct intrapleural targeted deltarasin delivery against experimental MPE, since chronic KRAS inhibition may result in marked toxicity. For this, C57BL/6 mice received pleural MC38 cells, followed by a single intrapleural injection of liposomal-encapsulated deltarasin (15 mg kg−1; one single dose equal to the daily intraperitoneal drug dose administration) or empty liposomes31,32 on day seven post-tumour cells. Interestingly, single-dose intrapleural liposomal deltarasin exhibited equal efficacy with repetitive intaperitoneal drug treatment, halting both MPE accumulation and CD11b+Gr1+ cell influx (Fig. 5d; Table 5). We finally cross-examined the effects of deltarasin and of a well-characterized neutralizing anti-CCL2 antibody6,7,33,34. For this, C57BL/6 mice received intrapleural PANO2 cells stably expressing KrasG12C isoforms 2A or 2B. After 4 or 14 days, respectively, mice started receiving daily intraperitoneal deltarasin (15 mg kg−1) or anti-CCL2 antibody (50 mg kg−1) every 3 days. Control mice received daily saline injections and IgG2a control antibody (50 mg kg−1) every 3 days. Interestingly, both treatments were equally effective in reducing MPE incidence and volume, as well as CD11b+Gr1+ cell accumulation (Fig. 5e; Table 5). These results indicated that deltarasin is effective in halting MPE induction by KRAS-mutant tumour cells and suggested that mutant KRAS-driven MPE in humans may also be actionable.
The dichotomous phenotype of primary and metastatic pleural tumours, some of which are associated with an MPE whereas others are not, is of paramount clinical importance, and prompted us to hypothesize that a causative molecular signature underlines MPE formation2,3,4. We show that cancer cells bearing different KRAS mutations cause MPE upon pleural dissemination and that mutant KRAS is important for experimental MPE development. Furthermore, that mutant KRAS-driven MPE is attributed to a CCL2-dependent signalling cascade that is necessary for the sequential translocation of CD11b+Gr1+ cells from the bone marrow to the spleen and the tumour-involved pleural cavity, where, in turn, these cells promote MPE formation. Proof-of-principle clinical data indicate that KRAS mutations are present in a substantial proportion of MPE patients in Europe and that they might be underestimated by automated sequencing analyses. Finally, we show that pharmacologic interception of this newly identified KRAS-driven, CCL2-mediated pathway to MPE can prevent MPE development.
The newly identified genotype-disease connection between mutant KRAS and MPE was corroborated using 12 different isogenic cellular systems of parental and daughter KRAS-modulated cell lines. In each and single one of these systems, mutant KRAS was required and sufficient for MPE. Particularly impressive was the switch of PANO2 cells upon KrasG12C2A expression from complete MPE incompetence during a month’s observation to acute and lethal MPE induction within 7 days. The same is true for benign HEK293T that were rendered MPE-proficient by isolated expression of KrasG12C2B per se. But is the proposed mutant KRAS-MPE link clinically relevant? KRAS mutations are not frequently found in human MPE as opposed to EGFR and EML4/ALK mutations8,9,10,11,12,13,14,15,16. First, we believe that KRAS mutations are not looked for because they are considered not actionable and mutually exclusive to EGFR mutations, notions that are currently being revisited8,9,10,11,12,13,14,15,16,35,36. We show the clinical data that indicate that KRAS mutations are frequent in European patients with MPE from lung adenocarcinoma and that they might be underappreciated. A recent study also showed how KRAS mutations can be missed in MPE samples, but persist in cultured cell lines derived from the same patients16, a finding recapitulated in patients from our centre. To this end, most MPE sequencing studies were performed in Asian populations with high EGFR and EML/ALK and low KRAS mutation frequencies8,9,10,11,12,13,14,15,16,35,36. A recent study of European patients with resected lung tumours clearly showed that KRAS mutations are linked with pleural spread19. Third, pleural tumours are diffuse-multifocal and probably multiclonal36 and it is conceivable that mutant KRAS MPE-initiating cells escape detection in focal pleural tumour tissue biopsies and low-volume pleural fluid aspirates.
We do not claim that KRAS mutations are the only ones that cause MPE in humans and postulate mutant KRAS effects to be class effects shared by all driver mutations aligned along the KRAS pathway, including EGFR, KRAS, PIK3CA, BRAF, MET, EML4/ALK, RET and others. To this end, mutant EGFR was recently shown to cause MPE when expressed in H1299 human lung adenocarcinoma cells17. However, possible pathogenic roles for other lung cancer drivers in MPE remain to be shown. Together with the advent of MPE sequencing techniques8,9,10,11,12,13,14,15,16, such developments could lead to targeted therapies for MPE in the near future. Moreover, MPE is a clinically heterogeneous set of diseases from a number of primary sites. KRAS mutations are more relevant to patients with lung, pancreatic, and colon cancers and leukaemias. In other tumors (that is, breast cancer) other mutations may be functionally involved in MPE formation (that is, HER2), a postulation that awaits experimental confirmation. To this end, future human studies aimed at identifying genotype–phenotype linkages in various tumours need to be tailored appropriately and need not rely on cross-sectional frequency observation design. Prospectively genotyped, case-matched, and longitudinally observed patient cohorts are more likely to give answers to questions such as the KRAS-MPE link proposed here.
In addition to the novel cancer genome-phenotype association, we further show here that mutant KRAS-driven MPE is mediated via CCL2-dependent paracrine signalling to CD11b+Gr1+ myeloid cells. The well-studied cell-autonomous effects of mutant KRAS conferring addictive proliferation advantages to the tumour cell35,37 may be complemented by this paracrine axis and may temporally precede its clinical manifestation, since mutant KRAS likely promotes pleural metastasis prior to MPE development18,19. As opposed to neutrophil chemoattractants such as CXCL1, CXCL2 (ref. 25), tumour-elaborated CCL2 is a potent monocyte/macrophage mobiliser promoting angiogenesis and metastasis34,38 and was identified here as the transcriptional target of mutant KRAS in tumour cells. This finding complements previous observations implicating CCL2 in mutant KRAS-driven inflammation in the lung epithelium39 and in MPE formation5,6. Interestingly, mutant HRAS also induces IL-8 signalling40 and the results imply that different RAS proteins may control distinct chemokine repertoires in order to mobilize defined myeloid cell subsets to tumour sites.
Our present and previous findings5,6,7 indicate that pleural tumour-originated CCL2 mobilizes two distinct cell populations from the bone marrow: mast cells and CD11b+Gr1+ cells41,42, both known to respond to CCL2 (refs 7, 25), to facilitate breast cancer metastasis to the lungs34, and to sustain tumour growth by promoting angiogenesis28. CD11b+Gr1+ cells were previously identified in MPE42 along with mast cells that were shown to promote MPE by fostering tumour growth and vascular permeability7. Here we show that the spleen is an important intermediate organ for MPE development, similar to other tumour models, with its marginal zone functioning as a reservoir for bone-marrow-derived CD11b+Gr1+ cell progenitors that are subsequently rapidly deployed to tumour sites43,44. Our experiments, in line with the work of others26,27,28,43,44 incriminate the spleen as a pro-tumour organ and suggest that the splenic environment is essential for CD11b+Gr1+ cell recruitment to MPE. As splenectomy provided marked protection to mice against incipient MPE, splenectomy at the time of pleurodesis or catheter placement may yield considerable benefit to patients with MPE, a notion worth exploring.
Finally, we present evidence that mutant KRAS-mediated MPE is actionable by the novel inhibitor of KRAS membrane transport deltarasin, lending hope for clinical targeting of the oncogene in the future30,35. Importantly, a CCL2 neutralizing antibody6,7,33,34 was as effective as deltarasin, strengthening the KRAS-CCL2 connection and indicating that intercepting downstream of mutant oncogene targets may be an alternative to their direct targeting. In addition to the clinical significance of KRAS and other driver mutations of lung and other cancers in MPE that needs to be established, open questions that remain include whether the hypoxic pleural environment impacts MPE development and whether it triggers phenotypic changes in pleural metastasized tumour cells, including the KRAS/CCL2 axis reported here.
In summary, we show that KRAS mutations are causally linked with MPE in mice. We also show that this link rests on a defined innate immune response and that it might be at play in humans with the condition. We believe that this work opens up avenues of potential progress towards aetiologic MPE therapy, by providing preclinical proof-of-concept data on immediate and feasible targeted interventions, such as splenectomy and KRAS and CCL2 blockade, which could provide meaningful benefits to patients with MPE in the future.
Human MPE samples from twenty patients with lung adenocarcinoma-associated MPE from Institution 1 were obtained and biobanked according to a prospectively placed, standardized and Institutional Ethics Committee-approved protocol (approval number 22699/21.11.2013) that abides by the Declaration of Helsinki. Written informed consent was obtained from each patient. Mouse experiments were carefully designed and were prospectively approved by the Veterinary Administration of the Prefecture of Western Greece (protocol approval numbers 3741/16.11.2010, 60291/3035/19.03.2012, and 118018/578/30.04.2014), and were conducted according to Directive 2010/63/EU (http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033: 0079:EN:PDF).
Evans’ blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay powder were from Sigma-Aldrich (St Louis, MO); D-luciferin was from Gold Biotechnology (St Louis, MO); Mouse Gene ST2.0 microarrays and relevant reagents were from Affymetrix (Santa Clara, CA); murine CCL2, CXCL1, and CXCL2 and human CCL2 ELISA kits were from Peprotech EC (London, UK); primer sets and antibodies are listed in Supplementary Tables 1 and 2, respectively; RAS activation assay was from Merck Millipore (Darmstadt, Germany); deltarasin was from MedChem Express (Princeton, NJ) and from Cayman Europe (Tallinn, Estonia); anti-mouse CCL2 neutralizing antibody, as well as IgG2a control antibody were from Oncology Discovery Research, Janssen R&D LLC (Radnor, PA)33,34; 1, 2-Distearoyl-sn-glycero-3-phosphocholine, phosphatidylglycerol and cholesterol were from Avanti Polar Lipids, Inc. (Alabaster, AL).
C57BL/6 (#000664), NOD/SCID (#001303), CAG.Luc.eGFP (#008450), and Ccr2−/− (#004999) mice from Jackson Laboratories (Bar Harbor, ME) were bred in the University of Patras Center for Animal Models of Disease. All experiments entailing murine cell lines were done using mice on the C57BL/6 background or CAG.Luc.eGFP mice backcrossed >F12 to the C57BL/6 background. All experiments entailing human cell lines were done using mice on the NOD/ShiLtJ background. Nine hundred and seventy-five sex-, weight (20–25 g)- and age (6–12 week)-matched male and female (50% of mice from each sex were enrolled in each experimental arm) experimental mice were used for these studies. The exact animal numbers per experiment are given in Tables 1, 2 and 5 and in the Legends to Figures.
C57BL/6 mouse B16F10 skin melanoma and PANO2 pancreatic and Lewis lung carcinomas (LLC), as well as human SKMEL2 skin melanoma, A549 lung and HT-29 colon adenocarcinomas were from the National Cancer Institute Tumour Repository (Frederick, MD); human HEK293T embryonic kidney cells were from the American Type Culture Collection (Manassas, VA); C57BL/6 mouse MC38 colon adenocarcinoma cells were a gift from Dr Barbara Fingleton (Vanderbilt University, Nashville, TN, USA)6,7,45, C57BL/6 mouse AE17 malignant pleural mesothelioma cells from Dr Y.C. Gary Lee (University of Western Australia, Perth, Australia)46, and human LTP A549 cells that have suffered chromosome Y loss from Dr Haralabos P. Kalofonos (University of Patras, Greece). Primary lung adenocarcinoma cells from C57BL/6 and FVB mice (CULA and FULA cells, respectively) were generated as described elsewhere23. Briefly, C57BL/6 and FVB mice received ten and four consecutive weekly intraperitoneal injections of urethane (1 g kg−1) and were killed ten months later. Lung tumours were isolated under sterile conditions, strained to single cell suspensions, and cultured for>100 passages over two years. Primary airway cells were derived by culturing stripped murine tracheal epithelium. Cell lines were authenticated annually using the short tandem repeat method, microarray, and Sanger sequencing and were tested for Mycoplasma Spp. biannually by PCR using designated primers (Supplementary Table 1). All cell lines were cultured at 37 °C in 5% CO2-95% air using full culture medium (DMEM supplemented with 10% FBS, 2 mM L-glutamine, 1 mM pyruvate, 100 U ml−1 penicillin, and 100 mg ml−1 streptomycin). For in vivo injections, cells were collected with trypsin, incubated with Trypan blue, counted by microscopy in a haemocytometer, their concentration was adjusted in PBS, and cell were injected through a left intercostal space or in the skin, as described elsewhere5,6,7. Only 95% viable cells were used for in vivo injections.
Sequencing plasmids and microarrays
Total cellular RNA was isolated using Trizol (Invitrogen, Thermo Fisher Scientific, Waltham, MA) followed by RNAeasy column purification and genomic DNA removal (Qiagen, Hilden, Germany). One μg purified total RNA was reverse transcribed using an Oligo(dT)18 primer and Superscript III (Invitrogen, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions. For sequencing reactions, Kras, Egfr, Braf and Pik3ca cDNAs (or parts of these cDNAs) were amplified in PCR reactions using the corresponding primers (Supplementary Table 1) and Phusion Hot Start Flex polymerase (New England Biolabs, Ipswich, MA). cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel, Düren, Germany) and were directly Sanger-sequenced with their corresponding forward and reverse primers by VBC Biotech (Vienna, Austria). For RNA interference, the following proprietary lentiviral shRNA pools of three were obtained from Santa Cruz Biotechnology (Palo Alto, CA): random control shRNA (shC, sc-108080-V), GFP control (sc-108084-V), and anti-Kras.shRNA (shKras, sc-33876-V). Anti-Kras lentiviral shRNA target sequences were: 5′-CTACAGGAAACAAGTAGTA-3′, 5′-GAACAGTAGACACGAAACA-3′ and 5′-CCATTCAGTTTCCATGTTA-3′. For this study, the following new plasmids were constructed in-house and were deposited with Addgene (https://www.addgene.org/Georgios_Stathopoulos/), accompanied by their full sequence files: (i) a pMIGR1-based puromycin resistance bicistronic retroviral expression vector (Addgene ID 64335) was constructed by replacing the eGFP sequences of pMIGR1 vector downstream of IRES with puromycin resistance sequences; (ii) a pMIGR1-based hygromycin resistance bicistronic retroviral expression vector (Addgene ID 64374) was constructed by replacing the eGFP sequences of pMIGR1 vector downstream of IRES with hygromycin resistance sequences. Both puromycin and hygromycin resistance genes were subcloned by restriction enzymes from available construct; (iii) the derivatives of these vectors, namely eGFP.retro.puro (Addgene ID 64336), eGFP.retro.hygro (Addgene ID 64375), eGFP.KRASwt-2B retro.puro (Addgene ID 64371), eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372), eGFP.KRASG12C-2B.retro.hygro (Addgene ID 64376), eGFP.KRASG12C-2A.retro.puro (Addgene ID 64373). Murine KrasG12C isoform 2A and 2B cDNAs were amplified using a first stand synthesis reverse transcription reaction from 1 μg total LLC RNA using specific primers (Supplementary Table 1) and Phusion Hot Start Flex polymerase (New England Biolabs, Ipswich, MA). Similarly, wild type Kras2B cDNA was amplified using a first stand synthesis reverse transcription reaction from 1 μg total PANO2 cellular RNA.The corresponding cDNAs were cloned into a peGFP.C1 vector (Clontech, Mountain View, CA) between BglII and EcoRI restriction sites, in-frame with eGFP and were verified by sequencing. The eGFP, as well as the wild type eGFP.KRAS2B and mutant eGFP.KRASG12C2A and 2B-encoding sequences were restricted out from the corresponding peGFP.C1-based constructs described above using enzymes AgeI and SmaI, were gel purified, and were subcloned into the (AgeI/HpaI-restricted) retroviral bicistronic expression vectors upstream of IRES-puromycin (ID 64336) and/or IRES-hygromycin (ID 64375) resistance genes, respectively. Retroviral particles were obtained by transfecting a 3 mm well of confluent HEK293T cells with the desired bicistronic retroviral vectors (expressing either eGFP or wild type or mutant eGFP.KRAS) along with VSV-G envelope expressing plasmid pMD2.G (Addgene ID12259) and pCMV-Gag-Pol expressing the retroviral structure proteins (Cell Biolabs Inc, San Diego, CA) at 1.5:1:1 mass stoichiometry via the CaCl2/BES method. After two days, the cultured medium (2 ml for each retroviral type) was collected and passed through a 45 μM filter to remove cellular debris. This supernatant material was supplemented with 8 ml medium and was subsequently overlaid on a 100 mm plate containing 70% confluent mouse cancer cells. After 48 h, the medium was removed and the cells were incubated with selection medium (full cell culture medium supplemented with either 2–10 μg ml−1 puromycin or 50–100 μg ml−1 hygromycin). Stable clones were selected and subcultured. All other cell lines were transfected with standard DNA amounts using X-Fect (Clontech, Mountain View, CA).
Experimental pleural carcinomatosis was induced by pleural delivery of 1.5 × 105 murine cancer cells, 106 human cancer cells or 3 × 106 HEK293T cells. MPE models and splenectomy have been described elsewhere5,6,7. For bioluminescence imaging, mice were serially imaged on a Xenogen Lumina II and data were analysed using Living Image v.4.2 (Perkin-Elmer, Waltham, MA), after delivery of 1 mg intravenous D-luciferin (Gold Biotechnology, St Louis, MO) by retro-orbital injection. For splenocyte give-back, spleens were removed under sterile conditions from CAG.Luc.eGFP donors (n=3 per group), 13 days after intrapleural injection with saline or tumour cells. Single-cell suspensions were prepared by passing spleens through 70 μm nylon cell strainers (BD Biosciences, Bedford, MA), followed by delivery of 100 μl saline containing 5 × 106 splenocytes to splenectomized hosts.
For induction of malignant pleural carcinomatosis with or without a MPE, mice were anaesthetized using isoflurane inhalation and received intrapleural injections of 100 μl PBS containing 150,000 murine, 1,000,000 human cancer cells or 3,000,000 HEK293T cells. Mice were killed when moribund for survival and pleural fluid and tumour analyses. Mice with pleural fluid volume ≥100 μl (equals the initial injection volume) were judged to have a MPE (‘wet’ pleural metastasis, that is, active pleural fluid exudation) and were subjected to pleural fluid aspiration, whereas animals with pleural fluid volume <100 μl were judged not to have a MPE (‘dry’ pleural carcinomatosis) and were subjected to pleural lavage. For this, 1 ml normal saline was injected intrapleurally and was withdrawn after 30 s. Following pleural fluid or lavage retrieval, the chest was opened and pleural tumours were stripped and weighed. For subcutaneous tumour formation, mice received 1,000,000 subcutaneous cancer cells and were followed longitudinally. Three vertical tumour dimensions (δ1, δ2 and δ3) were monitored serially and tumour volume was calculated using the formula π × δ1 × δ2 × δ3/6. Both models have been described elsewhere5,6,7.
Cytology histology and microscopy
Before further processing, MPE fluids were subjected to red blood cell lysis in ten-fold excess red blood cells lysis buffer (155 mM NH4Cl, 12 mM NaHCO3, 0,1 mM EDTA). Total pleural cell counts were determined microscopically on a haemocytometer and cytocentrifugal specimens (5 × 104 cells each) of total pleural fluid cells (or of CD45+CD11b+Gr1+ sorted cells) were fixed with methanol for 2 min. Cells were stained with May–Grünwald working solution (May–Grünwald stain in 1 mM Na2HPO4, 2.5 mM KH2PO4, pH=6.4) for 6 min, then with Giemsa working solution (Giemsa stain in 2 mM Na2HPO4, 5 mM KH2PO4, pH=6.4) for 40 min, then washed with H2O, and dried. Slides were mounted with Entellan (Merck Millipore, Darmstadt, Germany) and coverslipped and were microscopically analysed. For flow cytometry and fluorescence-activated cell sorting (FACS) staining, 106 nucleated pleural fluid cells suspended in 50 μl FACS buffer (PBS supplemented with 2% FBS and 0,1% NaN3) were stained with the indicated antibodies according to manufacturer’s instructions (Supplementary Table 2) for 20 min in the dark, were washed with FACS buffer from excess antibody, and were resuspended in 1 ml FACS buffer for further analysis. Spleens fixed in 10% formaldehyde overnight were embedded in paraffin and stored at room temperature. Five-micrometre paraffin sections were mounted on glass slides. Pleural tumours fixed in 10% paraffin or in 4% paraformaldehyde overnight were embedded in paraffin or in optimal cutting temperature (OCT; Sakura, Tokyo, Japan) and were stored at room temperature or −80 °C, respectively, till further analyses. Five-micrometre-thick paraffin or 10-μm-thick cryosections were mounted on glass slides. Sections were labelled using the indicated antibodies (Supplementary Table 2) and were counterstained with Hoechst 33258 or with Envision colour development system (Dako, Carpinteria, CA). Immunoreactivity was quantified as described previously5,6,7. Bright-field and fluorescent microscopy were carried out using either an AxioObserver D1 inverted microscope (Zeiss, Jena, Germany) or an SP5 confocal microscope (Leica, Heidelberg, Germany).
Flow cytometry and cell sorting
A CyFlow ML instrument with FloMax Software (Partec, Münster, Germany) was used for cell cytometry, sorting, and data analysis. CD45+CD11b+Gr1+ cells were identified, enumerated by true volumetric counting, and their total numbers were calculated as fraction of total MPE cell counts, or were sorted and collected in FACS buffer at 5 × 104 cells per ml.
In vitro cancer cell proliferation was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For this, 2 × 104 cells per well were plated onto 96-well plates. Daily thereafter, 15 μl of 5 mM MTT working solution in PBS was added to wells to be measured that day. The plate was incubated for 4 h at 37 °C in a 5% CO2 humidified incubator followed by addition of 100 μl acidified isopropanol per well for sediment solubilization and absorbance measurement at 492 nm on a MR-96 A photometer (Mindray, Shenzhen, China). For soft agar colony formation assay, 7.5 × 103 cells were plated in 60 mm culture vessels in semi-solid 0.7% agarose in full culture medium and were incubated for 30 days at 37 °C in a 5% CO2 humidified incubator. 2 ml fresh culture medium was added to each vessel biweekly. After incubation, 500 μl MTT working solution was added to each vessel and plates were dried, inverted, photographed and colonies were counted.
Vascular permeability assays
Mice with MPE received 0.8 mg intravenous Evans’ blue and were killed after 1 h for determination of MPE levels of the albumin-binding dye5,6,7. Intradermal injections of 50 μl cell-free pleural lavage or MPE were performed at different spots of the shaved dorsal mouse skin followed immediately by Evans’ blue injections as above, and euthanasia, skin inversion and imaging after one hour. The surface area of dye leak was determined using Fiji academic freeware (http://fiji.sc/Fiji), as described elsewhere5,6,7.
Chick chorioallantoic membrane assay
Fertilized White Leghorn chicken eggs, as soon as embryogenesis started, were placed for incubation under constant humidity at 37 °C. On day 4, a square window was opened in the shell and then sealed with adhesive tape. On day 9, a 1 cm2 rubber O-ring was placed on the surface of the CAM and 50 μl PBS or cell-free MPE or pleural lavage supernatants were added inside the ring’s restricted area. After 48 h, CAM tissues were fixed in Carson’s solution (saline-buffered formalin) and angiogenesis was evaluated using Fiji.
Real-time quantitative PCR and microarray hybridization and analysis
qPCR was performed using first strand synthesis reactions along with the indicated primers and KAPA SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems, Carlsbad, CA). The CT values from triplicate qPCR reactions were extracted from the StepOne cycler (Applied Biosystems, Carlsbad, CA) onto Excel spreadsheets and were analysed with the relative quantification method 2–ΔΔCT. The expression level of a given mRNA per sample/condition was determined relatively to reference gene mRNA levels. For microarray, triplicate cultures of 106 cells (for each cell line/condition) were subjected to RNA extraction as above. Five μg of pooled total RNA were tested for RNA quality on an ABI2000 bioanalyser (Agilent Technologies, Sta. Clara, CA), labelled, and hybridized to GeneChip Mouse Gene 1.0 or 2.0 ST arrays according to manufacturer’s instructions (Affymetrix, Sta. Clara, CA). For microarray analysis, the Affymetrix Expression Console was used (parameters: annotation confidence, full; summarization method: iter-PLIER include DABG; background: PM-GCBG; normalization method: none), followed by normalization of all arrays together using a Lowess multi-array algorithm. Intensity-dependent estimation of noise was used for statistical analysis of differential expression. Unsupervised hierarchical clustering of microarray data and Venn diagrams were performed using the MatLab 2014 program. Microarray data are publicly available at the GEO database (http://www.ncbi.nlm.nih.gov/geo/; Accession ID: GSE58190 and GSE85021).
RAS activation assay and immunoblotting
Total protein extracts were prepared by lysing 107 cultured cells in Mg2+ Lysis/Wash buffer (25 mM HEPES pH=7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% glycerol). RAS activation assay was performed with RAS Activation Assay Kit (Merck Millipore, Darmstadt, Germany) by incubating total cell lysates with Raf-1 Ras-Binding Domain (RBD)-coated agarose beads according to the manufacturer’s instructions. Total protein extracts and Raf-1 RBD agarose bead samples were analysed using 6–20% SDS–PAGE followed by standard blotting to PVDF membranes (Merck Millipore, Darmstadt, Germany). Membranes were incubated with primary antibodies, followed by incubation with the appropriate HRP-conjugated secondary antibodies at the manufacturers’ indicated dilutions (Supplementary Table 2), and were visualized by chemiluminescence film exposure, using the enhanced chemiluminescence substrate (Merck Millipore, Darmstadt, Germany). Full uncropped immunoblots are shown in Supplementary Figures 8–10.
Enzyme-linked immunosorbent assays (ELISA)
CCL2, CXCL1 and CXCL2 levels of cell culture supernatants, as well as CCL2 levels of cell-free MPE and corresponding sera were determined using dedicated murine and human ELISA kits according to the manufacturer’s instructions (Peprotech, London, UK and R&D, Minneapolis, MN).
Bone marrow transplantation
For adoptive bone marrow replacement, C57BL/6 hosts received ten million intravenous bone marrow cells flushed from the femurs and tibias of CAG.Luc.eGFP donors, 12 h after total-body irradiation (1,100 rad), as described elsewhere6,7. One mouse in each experiment was not engrafted (sentinel) and was observed till moribund between days 5 and 15 post-irradiation. The mice were left to recover at least for one month before subjecting them to experimentally induced MPE.
Liposome preparation and physicochemical characterization
Deltarasin-encapsulating liposomes were prepared by the DRV technique as described elsewhere31,32 by freeze drying 30 mg of empty DSPC/PG/Chol (9:1:5 (mol:mol:mol)) unilamelar sonicated vesicles with 1 ml of deltarasin solution (5 mg ml−1) in PBS, or plain PBS (for empty liposomes), followed by controlled re-hydration. The liposome size was decreased by extrusion though Lipo-so-fast extruder, polycarbonate membranes (Avestin Europe) with 400 nm diameter pore. Liposomes lipid concentration, size distribution and surface charge (zeta-sizer, Malvern) were estimated as reported elsewhere31,32, as well as drug encapsulation efficiency after measuring the non-liposomal drug absorption at 284 nm.
In vivo drug treatments
Treatments were initiated 4–14 days post-mouse and 14 days post-human pleural tumour cell injections. Deltarasin (15 mg kg−1 in 100 μl PBS), or 100 μl PBS were given daily intraperitoneally. Anti-mouse CCL2 or IgG2a control antibodies were delivered intraperitoneally at 50 mg kg−1 in 100 μl PBS every three days6,7. Deltarasin-encapsulating liposomes were prepared as described above and elsewhere31,32 and were delivered intrapleurally seven days post-intrapleural MC38 cells.
MPE cell concentrates from patients with lung adenocarcinoma-associated MPE from Institution 1 (n=20) were obtained and biobanked according to standard protocols, were handled similar to mouse MPE samples, and were subjected to RNA extraction, cDNA synthesis, PCR with human KRAS-specific primers (Supplementary Table 1), and direct Sanger sequencing. Some MPE cells were cultured for one month, tumor cell clones were picked and pooled, were inoculated into the flank of NOD/SCID mice to test their tumorigenicity, and were sequenced as above.
Sample size was calculated using G*power (http://www.gpower.hhu.de/;47) assuming errors α=0.05 and β=0.05, and effect size d=1.5. Experiments were done in a fractionated fashion until statistical significance (P<0.05 with α<0.05) was reached or ruled out (P>0.05 with β<0.05). No data were excluded from analyses and controls and intervention animals were enrolled as necessary per interim power analyses. Greater numbers of animals were added to follow-up experiments in groups where outliers increased the dispersion of the data, generating uneven experimental groups. Animals were allocated to treatments by alternation, and transgenic animals were enrolled case-control-wise. Data acquisition was blinded on samples previously coded by a non-blinded investigator. All data were examined for normality by Kolmogorov–Smirnov test and were found to be normally distributed. Values are given as frequencies, mean±s.e.m., or mean±s.d., as indicated. Sample size (n) refers to biological replicates, except from cell line qPCR data, where n refers to technical replicates. Differences in frequencies were examined by χ2 or Fischer’s exact tests, as appropriate. Differences in means were examined by two-tailed Student’s t-test, or one-way or two-way ANOVA with Bonferroni post-tests, as appropriate. Survival proportions were examined by Kaplan–Meier analysis using the log-rank test for overall and pairwise comparisons. P values are two-tailed, and P<0.05 was considered significant. Analyses and plots were done on Prism v5.0 (GraphPad Software, La Jolla, CA).
The microarray data have been deposited at the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/) under the accession codes GSE58190 and GSE85021). The vectors have been deposited at the Addgene plasmid depository (http://www.addgene.org/Georgios_Stathopoulos/) and plasmid ID’s are given in the text. Primary mouse lung adenocarcinoma cells were deposited at the Laboratory for Molecular Respiratory Carcinogenesis (LMRC) core cell line facility (http://www.lmrc.upatras.gr/) and are available upon request (email@example.com). The authors declare that all the other data supporting the findings of this study are available within the article and its Supplementary Information files and from the corresponding author upon reasonable request.
How to cite this article: Αgalioti, T. et al. Mutant KRAS promotes malignant pleural effusion formation. Nat. Commun. 8, 15205 doi: 10.1038/ncomms15205 (2017).
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This work was supported by European Research Council 2010 Starting Independent Investigator and 2015 Proof of Concept Grants (#260524 and #679345, respectively; to G.T.S.). We thank the University of Patras Centre for Animal Models of Disease and Advanced Light Microscopy Core for experimental support. The authors also thank Drs Barbara Fingleton (Vanderbilt University, Nashville, TN, U.S.A.), Y.C. Gary Lee (University of Western Australia, Perth, Australia), and Haralabos P. Kalofonos (University of Patras, Greece) for the kind provision of cell lines critical to this work.
L.A.S. is an employee of the company that produces the anti-CCL2 antibodies. The remaining authors declare no competing financial interests.
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Agalioti, T., Giannou, A., Krontira, A. et al. Mutant KRAS promotes malignant pleural effusion formation. Nat Commun 8, 15205 (2017). https://doi.org/10.1038/ncomms15205
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