(a) Frequency of thrombi formed in inferior vena cava 48 h after flow restriction in WT and N138G mice treated with or without anti-L-selectin F(ab′)2. For each experimental group, the number of mice forming thrombi relative to the total number of mice is shown. (b) Representative thrombi. Arrow indicates direction of blood flow. Bar, 1 mm. (c) Thrombus length and weight in WT and N138G mice treated with or without anti-L-selectin F(ab′)2. Each symbol represents an individual thrombus. Horizontal bars represent median values. (d) Representative cryosections of leukocyte-rich areas of thrombi stained with anti-Ly6G mAb to identify neutrophils and with DAPI to identify cell nuclei. Bar, 100 μm. (e) Stitched cryosections of entire thrombus stained with anti-Ly6G mAb. Bar, 1 mm. (f) Percentage of Ly6G-positive area per thrombus area in stitched sections. Data are mean±s.e.m. from seven mice per group. (g) Upper panel, representative western blot of thrombus lysates probed with antibodies to Ly6G and fibrin. The Aα, Bβ and γ chains of fibrin are marked. The full-length blot is shown in Supplementary Fig. 8. Lower panel, normalized densitometric ratio of Ly6G to fibrin Aα chain. Data are mean±s.d. from eight mice per group. *P<0.05; **P<0.01 (two-tailed Student’s t test).