Nanodiamonds are of interest as nontoxic substrates for targeted drug delivery and as highly biostable fluorescent markers for cellular tracking. Beyond optical techniques, however, options for noninvasive imaging of nanodiamonds in vivo are severely limited. Here, we demonstrate that the Overhauser effect, a proton–electron polarization transfer technique, can enable high-contrast magnetic resonance imaging (MRI) of nanodiamonds in water at room temperature and ultra-low magnetic field. The technique transfers spin polarization from paramagnetic impurities at nanodiamond surfaces to 1H spins in the surrounding water solution, creating MRI contrast on-demand. We examine the conditions required for maximum enhancement as well as the ultimate sensitivity of the technique. The ability to perform continuous in situ hyperpolarization via the Overhauser mechanism, in combination with the excellent in vivo stability of nanodiamond, raises the possibility of performing noninvasive in vivo tracking of nanodiamond over indefinitely long periods of time.
Nanoparticles are rapidly emerging as powerful theranostic substrates1 for the targeted delivery of vaccines2, chemotherapy agents3, immunotheraputics4, and as a means of tracking tumour distribution on whole-body scales5,6. Biocompatible nanodiamonds (NDs) are ideal examples, featuring surfaces that are readily functionalized to enable tissue growth and their selective uptake by disease processes7,8,9,10. Imaging NDs in vivo has been mostly limited to subcellular environments that are optically accessible11,12. Without imaging modalities beyond optical fluorescence realization of the full theranostic potential of ND to track and investigate complex disease processes, such as metastatic disease, is unlikely.
Magnetic resonance imaging (MRI) is the gold standard for noninvasive high-contrast imaging, but has proven ineffective for directly detecting NDs in vivo due to the low abundance and small gyromagnetic ratio of spin-1/2 13C nuclei that comprise the carbon lattice. Dynamic nuclear polarization (DNP) of the 13C nuclei at cryogenic temperatures can, in principle, overcome the inherently small nuclear spin polarization of diamond by boosting it some 10,000 times to enable MRI contrast from nanoparticle compounds13,14,15,16. Despite these prospects, hyperpolarized nuclei relax to their thermal polarization in a time that, for submicron particles, is short enough to limit the usefulness of the method in an imaging context13,17.
An alternative approach to tracking ND relies on functionalizing the ND surface with paramagnetic Gd(III) chelates to create complexes for imaging with conventional T1-weighted MRI18. However, this approach faces the challenges of a large background signal, and concern for the long-term toxicity of gadolinium-based compounds19.
Here, we demonstrate a different means of imaging and tracking water–ND solutions using Overhauser-enhanced MRI (OMRI)20,21,22,23. Operation at ultra-low magnetic field (ULF) enables efficient Overhauser polarization transfer between electronic and nuclear spins in a radio frequency (RF) regime compatible with in vivo use. RF pulsing of the electron paramagnetic resonance (EPR) transition between MRI signal acquisitions continually transfers spin polarization from the paramagnetic centres at the surface of ND to 1H nuclei in the surrounding water24. The presence of ND in the solution thus leads to an enhancement in the 1H MRI signal that can readily produce images with contrast sensitive to ND concentrations. The ability to perform in situ hyperpolarization overcomes the limitations imposed by short spin relaxation times of smaller particles and enables switchable tracking of ND solutions with no polarization transport losses over indefinite timescales. In addition to producing images to demonstrate this new approach, we investigate the conditions that lead to maximum sensitivity to the presence of ND, presenting data characterizing the efficiency of the Overhauser mechanism as a function of particle concentration and size. These results significantly enhance the theranostic capabilities of non-toxic, biofunctionalized ND, opening the possibility that MRI can be used to monitor and track ND compounds in vivo.
The Overhauser effect in ND solutions
Various types of ND were used in this study, including high-pressure high-temperature (HPHT), natural (NAT) and detonation (DET) NDs in sizes from 4 to 125 nm. We focus on results obtained from HPHT 18 nm and HPHT 125 nm NDs as typical representatives of the general behaviour observed. An air oxidization process, known to etch the ND surface, produces additional variants of NDs for comparison with the commercially sourced varieties25. Aqueous solutions of ND in deionized (DI) water were prepared using high-power probe sonication, with HPHT NDs exhibiting the most stability in solution. HPHT 125 nm solutions show no aggregation over a period of months and a zeta potential of −55 mV (see Methods for further details on ND preparation as well as Supplementary Note 1 and Supplementary Fig. 1a for zeta potential measurements).
The basis for detecting and imaging ND in solution is shown in Fig. 1a. Image contrast arises from the Overhauser effect, which as a starting point requires a reservoir of partially polarized electron spins26. Driving these electrons with a resonant AC magnetic field transfers spin polarization to the interacting 1H nuclei in the surrounding solution27,28. NDs provide such a reservoir in the form of paramagnetic impurities such as nitrogen vacancy centres, substitutional nitrogen (P1) centres and unpaired electrons at the nanoparticle surface29,30. We first characterize our NDs using EPR spectroscopy, determining their impurity content and suitability for Overhauser imaging.
The EPR spectra of our HPHT 18 nm ND solution is shown in Fig. 1b and is fit by a two-component spin-1/2 model comprising a broad (1.2 mT) component and a narrow (0.2 mT) component (solid lines in figure)31. Air oxidation of NDs reduces the amplitude of the broad component in the spectra, as shown in Fig. 1c, presumably by removing the paramagnetic centres at the surface. Our results are consistent with previous studies suggesting that the broad component is due to disordered dangling bonds at the surface of the ND with the narrow component arising from lattice defects in the crystalline core32. Other types of ND studied here demonstrate similar spectral components (see Supplementary Fig. 2 for further EPR data).
Having established that ND provides a paramagnetic reservoir suitable for the Overhauser effect, we turn now to address the additional conditions that must be satisfied to enable imaging. In Fig. 1d we show the energy level diagram of a system comprising an electron coupled to a 1H nucleus in an external magnetic field. When the EPR transition is pumped, the relative sizes of the cross relaxation transitions w0 and w2 will cause nuclei to accumulate in spin up or down states. This accumulation gives a nuclear enhancement , defined as the ensemble average of the z-component of the nuclear magnetization Mz over the nuclear magnetization under thermal equlibrium conditions M0, that is, . The enhancement generated by the Overhauser mechanism is a function of four parameters33:
where ρ is the coupling factor between electron and nuclear spins, f the leakage factor, s the saturation factor and γe and γn are the electron and nuclear gyromagnetic ratios. Addressing first the coupling factor, ρ, we note that when there is dipolar coupling but no hyperfine contact interaction between spins, ρ takes a positive value determined by the correlation time of the two spins, diffusion coefficients and the EPR frequency. Relatively long correlation times are expected at ND surfaces due to the formation of a nanophase of water with 1 nm thickness at the ND–water interface34. Assuming free diffusion of water at a distance of 1 nm from the ND surface, we follow refs 35, 36, 37, 38 and plot the field dependence of ρ for a calculated correlation time of 430 ns, as shown in Fig. 1e (see Supplementary Note 2 for details of calculation). Not surprisingly, given the long correlation time between spins, increasing the magnetic field above a few milli-Tesla rapidly suppresses the mutual flip–flip of dipolar coupled electron and nuclear spins and thus the nuclear enhancement possible via the Overhauser effect. Our choice of magnetic field for Overhauser imaging is thus constrained to the ULF regime, where serendipitously the frequency of the EPR field produces minimal heating from dielectric loss associated with water at 20 °C (ref. 39). Figure 1e also explains why previous DNP studies of ND solutions at 340 mT did not show Overhauser enhancement of freely diffusing water molecules in the bulk solution40. Instead, they showed solid effect DNP of 1H nuclei adsorbed to the ND surface.
To demonstrate that NDs can be detected via the Overhauser effect at ULF, we set B0=6.5 mT and apply an RF magnetic field at the EPR frequency of 190 MHz to an HPHT 125 nm, 100 mg ml−1 sample. The 1H signal from the water surrounding the ND is then detected through standard inductive nuclear magnetic resonance (NMR) techniques after the 1H system has reached equilibrium (see Methods). Under these conditions, we observe an enhancement of −4.0 in the 1H spin polarization when EPR power is applied, as shown in Fig. 2a.
Examining the enhancement produced by different types of ND, Fig. 2b shows the sensitivity of the Overhauser technique to nanoparticle concentration. We draw attention to the data for the HPHT 18 nm NDs, which indicates that at concentrations of 1 mg ml−1, a 33% change in 1H polarization of the solution can be observed. Natural NDs produce a small enhancement relative to HPHT NDs, probably due to a relatively low concentration of paramagnetic defects, as seen in their EPR spectra (see Supplementary Fig. 2 for spectra).
Having demonstrated that Overhauser enhancement is possible with ND, we return to equation (1) to further consider the conditions needed for optimal imaging. The saturation factor s describes the proportion of the EPR linewidth that is driven and takes a maximum value of 1 when the electron transitions are completely saturated at high RF power. To measure the EPR linewidth at ULF, we sweep B0 while driving electron transitions at 140 MHz. As shown in Fig. 2c, HPHT 18 nm and HPHT 125 nm solutions show a linewidth for the enhancement of ∼0.3 mT at a frequency consistent with the gyromagnetic ratio of a free electron. This result indicates that the paramagnetic centres responsible for the Overhauser effect can be fully saturated with a resonant AC magnetic field of magnitude 1 mT, which is easily achieved in our spectroscopic probe41. Accordingly, we observe that, for EPR powers above 30 W, the Overhauser enhancement saturates, (shown in Supplementary Fig. 1b), and the maximal saturation factor is reached.
The remaining parameter in equation (1) is the leakage factor f, which describes how effectively electrons relax the nuclear spin environment, taking a maximum value of 1 when all nuclear spin relaxation is via the paramagnetic solute. The leakage factor of a given solution can be calculated from:
where T1 is the spin–lattice relaxation time of 1H spins in ND solution, shown in Fig. 2d, and T01 is the spin–lattice relaxation time of the undoped solvent26. We note that ND solutions with shorter T1, and hence larger f, do not necessarily give a higher Overhauser enhancement as equation (1) would predict. For example, NAT 125 nm NDs show a T1 relaxivity more than double that of HPHT 125 nm NDs, despite showing a much smaller enhancement in Fig. 2b. Presumably, ρ is suppressed in the quasistatic nanophase by slow diffusion of water molecules and the increased paramagnetic nuclear relaxation rate40. 1H nuclei ‘trapped’ in the nanophase will experience rapid spin–lattice relaxation, giving the f we observe and an overall enhancement that depends on the specifics of each ND surface. A detailed understanding of dynamics in the nanophase compared to freely diffusing bulk water is thus crucial to calculation of the factors in equation (1) (see Supplementary Note 3 for further discussion).
Solutions prepared with air-oxidized ND consistently exhibit reduced enhancements and higher T1 relaxivity, as shown for 18 nm HPHT air-oxidized NDs in Fig. 2b,d. The increased nuclear spin–lattice relaxation rate will contribute to a reduction in Overhauser enhancement and we speculate that the enhancement is further reduced due to a lower concentration of paramagnetic centres after removal of surface impurities by air oxidation.
Overhauser-enhanced MRI with ND
With conditions that lead to a significant Overhauser enhancement now established, we demonstrate this approach as the basis for detecting ND solutions using ultra-low-field MRI. Imaging is performed using a custom proton–electron, double resonant probe in an open-access, low-field, human MRI scanner operating at a B0 of 6.5 mT (ref. 42). To display the MRI contrast possible between a ND solution and water we make use of the phantom illustrated in Fig. 3a, which consists of glass vials filled with 500 μl of either DI water or aqueous solutions of HPHT 125 nm ND at 100 mg ml−1 and is organized in a diamond-shaped pattern.
MRI at ultra-low field of the phantom was performed using a high-efficiency balanced steady-state free precession (bSSFP) MRI sequence in which 1/3 of the imaging time is spent acquiring signal (see Methods for details)43. Although good spatial resolution is achieved, no discernible contrast is evident between ND solution vials and water vials, as shown in Fig. 3b. This is not surprising given that contrast using the bSSFP sequence is produced via 1H concentration weighted by the ratio T2/T1, which is approximately equal for all vials in the phantom (see Fig. 2d for T1 and Supplementary Fig. 1c for T2). We note that obtaining relaxation contrast with bSSFP at ULF is usually challenging, as when B0→0, it is a general result that T2/T1→1 (ref. 44).
The phantom was then imaged with an OMRI bSSFP sequence, as shown in Fig. 3c. The OMRI bSSFP sequence is equivalent to the regular bSSFP sequence, except the EPR transition of the ND solution is driven during the phase encode period24. The maximum time period without Overhauser saturation in our OMRI bSSFP sequence is 28 ms. As this is much shorter than the T1 and T2 of the ND solution, the polarization approaches a steady state during OMRI bSSFP, ensuring that hyperpolarized signal is continually present for acquisition. The appearance of water vials in the OMRI bSSFP image is unchanged from the regular bSSFP image. However, the ND solutions demonstrate significant relative contrast, with a change in magnitude and inversion of signal phase, as a result of the negative enhancement from the Overhauser effect. The switchable nature of the Overhauser contrast allows us to take the difference of the signal in MRI and OMRI images to generate the image in Fig. 3d. Such a difference image suppresses the background signal, clearly showing the spatial distribution of NDs.
Having demonstrated ND imaging with OMRI, we now consider the sensitivity of the technique in our current Overhauser setup. We calculate the signal-to-noise ratio (SNR) as the magnitude of the MRI signal in a region of interest divided by the root mean square value of the background signal. In Fig. 4a we show the schematic of a phantom containing vials with various concentrations of HPHT 18 nm ND in a container of water. This phantom is imaged with bSSFP, as shown in Fig. 4b. The vials in this image have an SNR of 43, with the glass vial walls clearly outlining their positions. Next, we define the contrast-to-noise ratio (CNR) as the difference in signal between MRI and OMRI scans in a region of interest divided by the root mean square value of the background signal. Taking the subsequent Overhauser scan, shown in Fig. 4c and resulting difference image, shown in Fig. 4d, clearly shows the presence of ND at concentrations of 10, 3 and 1 mg ml−1 with CNR values of 27, 18 and 9, respectively.
Images of the 1 mg ml−1 vial in Fig. 4 were acquired with 23 μg of ND per pixel, or a particle molar sensitivity of 150 nM for 18 nm particles. We note that this particle mass sensitivity is equivalent to that reported for other hyperpolarized MRI particle imaging modalities17.
NDs are non-toxic at high concentrations and resist in vivo degradation for periods of over a month45. Thus, the results presented here illustrate the potential of ND OMRI as a practical methodology for long-term biological imaging, providing new types of contrast and functionality. All imaging was performed on systems designed for in vivo OMRI with RF powers acceptable for use in vivo46, raising the possibility of biological applications. In particular, NDs may be of diagnostic use for diseased organs where nanoparticle accumulation can be an effective marker of pathology, such as the brain47, liver48 and lymph nodes2. Current diagnostic methodologies, using the properties of iron oxide nanoparticles49, suffer from the long biodistribution times of nanoparticles as the precomparison scan is taken before nanoparticle administration. In the resulting interval before a postcomparison scan various types of biological noise are introduced that make difference imaging infeasible50. The ability to perform interleaved MRI and OMRI scans with ND could overcome this limitation.
We have demonstrated sensitivity to ND at concentrations as low as 1 mg ml−1. An upgraded version of our scanner with higher strength imaging gradients will enable slice selection without compromise to the bSSFP acquisition protocol. Hence, we now anticipate sensitivity changes from the implementation of slice selection. The SNR in a slice-selected image is given by SNR=κVvoxel, where Vvoxel is the volume of a voxel, tacq is the total acquisition time and κ is a constant that depends on the magnetic field strength, hardware sensitivity, imaging sequence and acquisition parameters as well as the composition and spin–relaxation properties of the material being imaged51. Hence, based on the measurements in Fig. 4, for the same acquistion time and slice selection in a 5 mm slab with 1 mm × 1 mm pixel size, we calculate that HPHT 18 nm ND at 1 mg ml−1 will be on the threshold of detectability with a CNR of 2. For higher nanoparticle concentrations, imaging times could be significantly accelerated. For example, HPHT 18 nm ND at 10 mg ml−1 will have a CNR of 2, with 5 mm3 voxels and a total MRI and OMRI acquisition time of 2.5 min.
The long-term clearance of nanoparticles is of interest for assessing biocompatibility. Studying the retention of NDs in the liver at present requires organ harvesting, which limits long-term studies45,52. We estimate that for NDs to be present at 1 mg ml−1 in a 3 ml mouse liver53 would require injection of 5 mg of ND into a 20 g mouse, assuming 60% accumulation in the liver53,54. This dose is significant as it is a factor of 30 lower than that used in a recent in vivo demonstration of hyperpolarized silicon microparticle imaging17. Nanoparticle accumulation could be noninvasively imaged at this concentration with 5 mm3 voxels in the 3,000 mm3 liver. This would provide a long-term probe of the fate of nanoparticles in the liver, with significant scope for increasing the voxel size or acquisition time if increased sensitivity is required.
Given that nanoparticles about 25 nm in size are known to preferentially accumulate only in healthy lymphatic tissue2, the ability to detect and image ND with OMRI may also enable isolation of disease in swollen lymph glands, avoiding the need for biopsy55,56,57. Such a technique could prove useful for the diagnosis of lymph node tumours, which is vital to the treatment of metastatic prostate cancer6.
The 1H enhancement we observe with the Overhauser effect is approximately two orders of magnitude larger, when accounting for ND concentration, than seen with solid effect hyperpolarization of water molecules adsorbed to ND surfaces40. Further, there is potential to increase the Overhauser enhancement towards the theoretical maximum of −330 (ref. 26), over 80 times larger than seen here, by modification of the ND surface. Tailored NDs with impurities selected to remove alternate spin–lattice relaxation mechanisms could be surface treated to increase diffusion of water at the nanosolid–liquid interface58,59, maximizing the coupling and leakage factors in equation (1). Likely, other nanoparticles that display the Overhauser effect in solution also exist. However, identification of these nanoparticles is nontrivial because of significant variation in the surface defects and the hydrophilicity of nanoparticles.
As Overhauser contrast arises via interactions at the nanoparticle surface, we recognize that surface functionalization for targeted molecular imaging must complement the observed enhancement. In this way, therapeutic agents attached to the surface could suppress the Overhauser effect by increasing the distance between radicals at their surface and free water, leaving them ‘dark’ in OMRI scans60. After targeted drug release, the Overhauser effect could return to normal, showing up ‘bright’ with OMRI and enabling effective tracking of the site of drug delivery. The dependence of Overhauser enhancement on diffusion may also allow the technique to be used as a probe of localized hydration dynamics35. The OMRI approach may also enable the hyperpolarization of fluids flowing across the surface of diamond nanostructures61,62,63.
In conclusion, we have used recent advances in ULF MRI to extend the usefulness of OMRI to nanoparticle imaging. The ability to noninvasively image biocompatible NDs with switchable contrast at biologically relevant concentrations is promising for a range of diagnostic applications. Switchable contrast allows suppression of the background signal present in other T1- and T2-based nanoparticle MRI modalities18,64,65. Furthermore, the long-term biological stability of NDs in vivo, as well as the unlimited repeatability of the hyperpolarization sequence, raises the possibility of imaging metabolic processes over dramatically longer timescales than is possible with ex situ hyperpolarization techniques13,17.
ND solution preparation
NDs used in this study were sourced from Microdiamant (Switzerland). ND types used were: monocrystalline, synthetic HPHT NDs in 18 nm (0–30 nm, median diameter 18 nm) and 125 nm (0–250 nm, median diameter 125 nm) sizes; monocrystalline, 125 nm NAT NDs (0–250 nm, median diameter 125 nm); and polycrystalline DET ND (cluster size 250–1,000 nm, median 500 nm; individual particle size 4–8 nm). Size specifications were provided by the manufacturer. Air-oxidized NDs were prepared by placing them in a furnace at standard pressure for 1 h at 550 °C after an initial temperature ramp25. ND samples were mixed with DI water and sonicated with a Branson probe sonicator at 120 W and 50% duty cycle for 40 min to disaggregate ND clusters.
Particle size and zeta potential measurements were performed on ND solutions in a Beckman Coulter Delsa Nano C Particle Analyzer. Particle size measurements confirmed that monocrystalline NDs were well dispersed in water after sonication. Particle sizes of monocrystalline NDs were found to be consistent with manufacturer specifications. DET NDs still displayed some clustering and inconsistent particle size in solution after probe sonication.
CW EPR measurements were performed on 100 mg ml−1 ND samples in a Bruker ElexSys E500 EPR system. Modulation frequency was 100 kHz at an amplitude of 0.1 G and incident microwave power of 0.6725, mW. Sample volumes in the cavity were kept consistent to allow comparison of relative peak heights. Individual EPR components were simulated in EasySpin31 and a least-squares analysis was used to find the best fit to the data while varying g-factor, linewidth and amplitude.
Spectroscopic measurements at 6.5 mT
The 1H enhancement of ND solutions was measured by saturating the EPR transition at 190 MHz for a period of 5T1 using 31 W of RF power before a standard NMR FID acquisition at 276 kHz. The enhancement is given as the ratio of the magnitude of the hyperpolarized FID to the magnitude an FID taken at thermal equilibrium with no EPR power. A high filling factor Alderman–Grant resonator was used for EPR with a solenoid-producing orthogonal B1 used for NMR detection. T1 relaxation times were measured using a conventional inversion recovery acquisition and fit with a least-squares analysis.
DNP linewidth measurements
The linewidth of the Overhauser effect was studied by measuring the 1H enhancement at various magnetic field strengths while driving the EPR resonator at a frequency of 140 MHz and 25 W. The EPR frequency was lowered from 190 MHz to capture the enhancement profile either side of the peak without exceeding the maximum field accessible in our ULF magnet. 1H enhancement was measured as the magnetic field was stepped between 4 and 7 mT. NMR detection was performed at the 1H resonance for any given field strength, with a low Q solenoidal coil.
Imaging was performed at 6.5 mT in our ultra-low-field MRI scanner42 using a bSSFP OMRI sequence at room temperature24. The homebuilt imaging probe consists of an Alderman–Grant resonator (EPR: 190 MHz) and a solenoid (1H: 276 kHz). The EPR resonator was pulsed on during the phase encode steps, with 69 W delivered to the EPR resonator at a duty cycle of 52%. Gradient strength was a maximum of 1 mT m−1 (see Supplementary Note 4 and Supplementary Fig. 3 for further imaging sequence and probe details). Images in Figs 3 and 4 were acquired with a 256 × 40 matrix size and cropped. Data were acquired in two dimensions with a pixel size of 1.0 mm × 0.76 mm over a 30 mm × 30 mm field of view and interpolated by zero filling and Gaussian filtering in k-space to give 0.25 mm × 0.19 mm pixels. The phantom thickness was 20 and 30 mm in Figs 3 and 4, respectively. The standard MRI images were acquired with 200 averages (11 min 24 s). The OMRI images in Figs 3c and 4c were acquired with 80 averages (4 min 14 s) and 200 averages (11 min 24 s), respectively. Image magnitude was scaled for an accurate comparison between scans with different numbers of averages. Pixels in Fig. 4 with a magnitude less than five times the root mean square value of the background signal were thresholded to zero.
The authors declare that the data supporting the findings of this study are available within the article and its Supplementary Information. Raw data is available from the corresponding author on request.
How to cite this article: Waddington, D. E. J. et al. Nanodiamond-enhanced MRI via in situ hyperpolarization. Nat. Commun. 8, 15118 doi: 10.1038/ncomms15118 (2017).
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Min, Y., Caster, J. M., Eblan, M. J. & Wang, A. Z. Clinical translation of nanomedicine. Chem. Rev. 115, 11147–11190 (2015).
Reddy, S. T. et al. Exploiting lymphatic transport and complement activation in nanoparticle vaccines. Nat. Biotechnol. 25, 1159–1164 (2007).
Gaur, S. et al. Pharmacodynamic and pharmacogenomic study of the nanoparticle conjugate of camptothecin CRLX101 for the treatment of cancer. Nanomed. Nanotechnol. 10, 1477–1486 (2014).
Almeida, J. P. M., Figueroa, E. R. & Drezek, R. A. Gold nanoparticle mediated cancer immunotherapy. Nanomed. Nanotechnol. 10, 503–514 (2014).
Liu, Y. et al. Theranostic near-infrared fluorescent nanoplatform for imaging and systemic siRNA delivery to metastatic anaplastic thyroid cancer. Proc. Natl Acad. Sci. USA 113, 7750–7755 (2016).
Harisinghani, M. G. et al. Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. N. Engl. J. Med. 348, 2491–2499 (2003).
Chow, E. K. et al. Nanodiamond therapeutic delivery agents mediate enhanced chemoresistant tumor treatment. Sci. Transl. Med. 3, 73ra21 (2011).
Xi, G. et al. Convection-enhanced delivery of nanodiamond drug delivery platforms for intracranial tumor treatment. Nanomed. Nanotechnol. 10, 381–391 (2014).
Thalhammer, A., Edgington, R. J., Cingolani, L. A., Schoepfer, R. & Jackman, R. B. The use of nanodiamond monolayer coatings to promote the formation of functional neuronal networks. Biomaterials 31, 2097–2104 (2010).
Zhang, Q. et al. Fluorescent PLLA-nanodiamond composites for bone tissue engineering. Biomaterials 32, 87–94 (2011).
Schrand, A. M. et al. Are diamond nanoparticles cytotoxic? J. Phys. Chem. B 111, 2–7 (2007).
McGuinness, L. P. et al. Quantum measurement and orientation tracking of fluorescent nanodiamonds inside living cells. Nat. Nanotechnol. 6, 358–363 (2011).
Rej, E., Gaebel, T., Boele, T., Waddington, D. E. J. & Reilly, D. J. Hyperpolarized nanodiamond with long spin–relaxation times. Nat. Commun. 6, 8459 (2015).
Casabianca, L. B., Shames, A. I., Panich, A. M., Shenderova, O. & Frydman, L. Factors affecting DNP NMR in polycrystalline diamond samples. J. Phys. Chem. C 115, 19041–19048 (2011).
Dutta, P., Martinez, G. V. & Gillies, R. J. Nanodiamond as a new hyperpolarizing agent and its 13C MRS. J. Phys. Chem. Lett. 5, 597–600 (2014).
Bretschneider, C. O. et al. On the potential of dynamic nuclear polarization enhanced diamonds in solid-state and dissolution 13C NMR spectroscopy. Chem. Phys. Chem. 17, 2691–2701 (2016).
Cassidy, M. C., Chan, H. R., Ross, B. D., Bhattacharya, P. K. & Marcus, C. M. In vivo magnetic resonance imaging of hyperpolarized silicon particles. Nat. Nanotechnol. 8, 363–368 (2013).
Manus, L. M. et al. Gd(III)-nanodiamond conjugates for MRI contrast enhancement. Nano Lett. 10, 484–489 (2010).
McDonald, R. J. et al. Intracranial gadolinium deposition after contrast-enhanced MR imaging. Radiology 275, 772–782 (2015).
Lurie, D. J., Li, H., Petryakov, S. & Zweier, J. L. Development of a PEDRI free-radical imager using a 0.38 T clinical MRI system. Magn. Reson. Med. 47, 181–186 (2002).
Golman, K., Leunbach, I., Petersson, J. S., Holz, D. & Overweg, J. Overhauser-enhanced MRI. Acad. Radiol. 9, (Suppl 1): S104–S108 (2002).
Koonjoo, N. et al. In vivo Overhauser-enhanced MRI of proteolytic activity. Contrast Media Mol. Imag. 9, 363–371 (2014).
Ichikawa, K. & Yasukawa, K. Imaging in vivo redox status in high spatial resolution with OMRI. Free Radical Res. 46, 1004–1010 (2012).
Sarracanie, M., Armstrong, B. D., Stockmann, J. & Rosen, M. S. High speed 3D Overhauser-enhanced MRI using combined b-SSFP and compressed sensing. Magn. Reson. Med. 71, 735–745 (2013).
Gaebel, T. et al. Size-reduction of nanodiamonds via air oxidation. Diam. Rel. Mater. 21, 28–32 (2012).
Ravera, E., Luchinat, C. & Parigi, G. Basic facts and perspectives of Overhauser DNP NMR. J. Magn. Reson. 264, 78–87 (2016).
Clarkson, R. B. et al. Electron paramagnetic resonance and dynamic nuclear polarization of char suspensions: surface science and oximetry. Phys. Med. Biol. 43, 1907–1920 (1998).
Ardenkjaer-Larsen, J. H. et al. EPR and DNP properties of certain novel single electron contrast agents intended for oximetric imaging. J. Magn. Reson. 133, MN981438 (1998).
Panich, A. M., Sergeev, N. A., Shames, A. I., Osipov, V. Y. & Boudou, J. P. Size dependence of 13C nuclear spin–lattice relaxation in micro- and nanodiamonds. J. Phys. Condens. Matter 27, 72203 (2015).
Cui, J.-F., Fang, X.-W. & Schmidt-Rohr, K. Quantification of C=C and C=O surface carbons in detonation nanodiamond by NMR. J. Phys. Chem. C 118, 9621–9627 (2014).
Stoll, S. & Schweiger, A. EasySpin, a comprehensive software package for spectral simulation and analysis in EPR. J. Magn. Reson. 178, 42–55 (2006).
Yavkin, B. V., Mamin, G. V., Gafurov, M. R. & Orlinskii, S. B. Size-dependent concentration of N0 paramagnetic centres in HPHT nanodiamonds. Magn. Reson. Solids 17, 15101 (2015).
Günther, U. L. Dynamic nuclear hyperpolarization in liquids. Top. Curr. Chem. 335, 23–69 (2011).
Korobov, M. V., Avramenko, N. V., Bogachev, A. G., Rozhkova, N. N. & Osawa, E. Nanophase of water in nano-diamond gel. J. Phys. Chem. C 111, 7330–7334 (2007).
Armstrong, B. D. & Han, S. Overhauser dynamic nuclear polarization to study local water dynamics. J. Am. Chem. Soc. 131, 4641–4647 (2009).
Tóth, É., Helm, L. & Merbach, A. E. Relaxivity of MRI contrast agents. Top. Curr. Chem. 221, 61–101 (2002).
Hwang, L.-P. & Freed, J. H. Dynamic effects of pair correlation functions on spin relaxation by translational diffusion in liquids. J. Chem. Phys. 63, 4017–4025 (1975).
Freed, J. Dynamic effects of pair correlation functions on spin relaxation by translational diffusion in liquids. II. Finite jumps and independent T1 processes. J. Chem. Phys. 68, 4034–4037 (1978).
Gabriel, C., Gabriel, S., Grant, E. H., Halstead, B. S. J. & Mingos, D. M. P. Dielectric parameters relevant to microwave dielectric heating. Chem. Soc. Rev. 27, 213–223 (1998).
Rej, E., Gaebel, T., Waddington, D. E. J. & Reilly, D. J. Hyperpolarized nanodiamond surfaces. J. Am. Chem. Soc. 139, 193–199 (2017).
Mispelter, J., Mikaela, L. & Briguet, A. NMR Probeheads for Biophysical and Biomedical Experiments Imperial College Press (2006).
Sarracanie, M. et al. Low-cost high-performance MRI. Sci. Rep. 5, 15177 (2015).
Scheffler, K. & Lehnhardt, S. Principles and applications of balanced SSFP techniques. Eur. Radiol. 13, 2409–2418 (2003).
Bottomley, P. A., Foster, T. H., Argersinger, R. E. & Pfeifer, L. M. A review of normal tissue hydrogen NMR relaxation times and relaxation mechanisms from 1–100 MHz: dependence on tissue type, NMR frequency, temperature, species, excision, and age. Med. Phys. 11, 425–448 (1984).
Vaijayanthimala, V. et al. The long-term stability and biocompatibility of fluorescent nanodiamond as an in vivo contrast agent. Biomaterials 33, 7794–7802 (2012).
Massot, P. et al. In vivo high-resolution 3D overhauser-enhanced MRI in mice at 0.2 T. Contrast Media Mol. Imaging 7, 45–50 (2012).
Saraiva, C. et al. Nanoparticle-mediated brain drug delivery: overcoming blood–brain barrier to treat neurodegenerative diseases. J. Control Rel. 235, 34–47 (2016).
Blanco, E., Shen, H. & Ferrari, M. Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat. Biotechnol. 33, 941–951 (2015).
Rosen, J. E., Chan, L., Shieh, D.-B. & Gu, F. X. Iron oxide nanoparticles for targeted cancer imaging and diagnostics. Nanomedicine 8, 275–290 (2012).
Zhu, B. et al. Selective magnetic resonance imaging of magnetic nanoparticles by acoustically induced rotary saturation. Magn. Reson. Med. 75, 97–106 (2016).
Haacke, E. M., Brown, R. W., Thompson, M. R. & Venkatesan, R. Magnetic Resonance Imaging: Physical Principles and Sequence Design Wiley (1999).
Moore, L. et al. Biocompatibility assessment of detonation nanodiamond in non-human primates and rats using histological, hematologic, and urine analysis. ACS Nano 10, 7385–7400 (2016).
Yuan, Y., Chen, Y., Liu, J. H., Wang, H. & Liu, Y. Biodistribution and fate of nanodiamonds in vivo. Diam. Rel. Mater. 18, 95–100 (2009).
Zhang, X. et al. Biodistribution and toxicity of nanodiamonds in mice after intratracheal instillation. Toxicol. Lett. 198, 237–243 (2010).
Liersch, R., Hirakawa, S., Berdel, W. E., Mesters, R. M. & Detmar, M. Induced lymphatic sinus hyperplasia in sentinel lymph nodes by VEGF-C as the earliest premetastatic indicator. Int. J. Oncol. 41, 2073–2078 (2012).
Zbinden, G. Fine-Needle Aspiration Biopsy of the Rat Liver: Cytological, Cytochemical and Biochemical Methods Pergamon Press (1980).
Trencsenyi, G. et al. Metastatic hepatocarcinoma He/De tumor model in rat. J. Cancer 5, 548–558 (2014).
Mochalin, V. N., Shenderova, O., Ho, D. & Gogotsi, Y. The properties and applications of nanodiamonds. Nat. Nanotechnol. 7, 11–23 (2012).
Duan, X. et al. Surface-tailored nanodiamonds as excellent metal-free catalysts for organic oxidation. Carbon 103, 404–411 (2016).
Mi, P. et al. A pH-activatable nanoparticle with signal-amplification capabilities for non-invasive imaging of tumour malignancy. Nat. Nanotechnol. 11, 724–730 (2016).
Lingwood, M. D., Siaw, T. A., Chan, H. R. & Ross, B. D. Hyperpolarized water as an MR imaging contrast agent: feasibility of in vivo imaging in a rat model. Radiology 265, 418–425 (2012).
Abrams, D., Trusheim, M. E., Englund, D. R., Shattuck, M. D. & Meriles, C. A. Dynamic nuclear spin polarization of liquids and gases in contact with nanostructured diamond. Nano Lett. 14, 2471–2478 (2014).
Meriles, C. A. & Doherty, M. W. Generation of spin-polarized currents via cross-relaxation with dynamically pumped paramagnetic impurities. Appl. Phys. Lett. 105, 022403 (2014).
Bouchard, L.-S. et al. Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles. Proc. Natl Acad. Sci. USA 106, 4085–4089 (2009).
Xing, Y. & Dai, L. Nanodiamonds for nanomedicine. Nanomedicine UK 4, 207–218 (2009).
We thank B.D. Armstrong for valuable discussions. This work was supported by the Australian Research Council Centre of Excellence Scheme (Grant No. EQuS CE110001013) ARC DP1094439, the Lockheed Martin Corporation, US Army Medical Research and Materiel Command (USAMRMC), Defense Medical Research and Development Program (DMRDP) award W81XWH-11-2-0076 (DM09094) and the US Army Research Office (ARO) Multidisciplinary University Research Initiative (MURI) award W911NF-15-1-0548. D.E.J.W. was supported by ANSTO and the Australian-American Fulbright Commison. N.S. was supported by the Swiss National Science Foundation (P300P2_147768).
The authors declare no competing financial interests.
About this article
Cite this article
Waddington, D., Sarracanie, M., Zhang, H. et al. Nanodiamond-enhanced MRI via in situ hyperpolarization. Nat Commun 8, 15118 (2017). https://doi.org/10.1038/ncomms15118
Solid-state 13C NMR of carbon nanostructures (milled graphite, graphene, carbon nanotubes, nanodiamonds, fullerenes) in 2000–2019: a mini-review
Fullerenes, Nanotubes and Carbon Nanostructures (2020)
Physical Review B (2020)
Computational study on the electronic g-tensors of hydrophilic and hydrophobic nanodiamonds interacting with water
The Journal of Chemical Physics (2020)
Nature Communications (2019)
Enhancement of nuclear spin coherence times by driving dynamic nuclear polarization at defect centers in solids
Physical Review B (2019)