(a) HPRT mutations in scrambled control-, shTET2- and shTET2+TET2opt HeLa cells. (b) Proportions of Tet2−/− tumour transitions, transversions and indels. (c) Comparison of numbers of nonsynonymous somatic mutations by WES MDS and MDS/MPN cases with (n=41) and without (n=154) TET2 mutations (P=0.001, Wilcoxon’s rank-sum test). (d) Comparison of numbers of nonsynonymous somatic mutations in AML cases with and without TET2 mutations (P=0.04, Wilcoxon’s rank-sum test) available only for primary AMLs through TCGA. Box plots: upper whisker, maximum value less than 75%+1.5 × IQR; upper box end, 75%; bar, 50%; lower box end, 25%; lower whisker, least value more than 25%−1. × IQR. IQR, interquartile range.