Figure 1 : AP expression in mouse postnatal skeletal muscle.

From: Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells

Figure 1

(ad) Immunofluorescence analysis on 1-month-old mouse muscle sections shows localization of AP within vessel-associated cells. (a) AP+ cells are stained in red, nuclei are stained in blue by DAPI (b) NG2+ cells are stained in green, (c) Ve-Cadherin+ cells are stained in cyan, (d) merged image. (eh) AP is expressed by PDGFRβ+/SMA+ pericytes. (e) AP+ cells are stained in red, nuclei are stained in grey by DAPI, (f) PDGFRβ+ cells are stained in green, nuclei are stained in grey by DAPI (g) SMA+ cells are stained in blue and nuclei in grey by DAPI, (h) merged image. (il) AP is expressed by Pecam+ endothelial cells, surrounded by an AP-negative pericyte. (i) AP+ cells are stained in red, nuclei are stained in grey by DAPI, (j) Pecam+ cells are stained in green, nuclei are stained in grey by DAPI, (k) NG2+ cells are stained in blue, nuclei are stained in grey by DAPI, (l) merged image. (m) Immunostained TA muscle (15-days-old) shows a Pax7+ satellite cell (green arrow), and an AP+ vessel-associated cell (red arrow); Laminin in magenta. We counted 170 AP+/Pax7− cells and 102 AP−/Pax7+ cells in TA muscle and 438 AP+/Pax7− cells and 138 AP−/Pax7+ cells in Soleus muscle: no co-localization is detected. (n,o) Myf5nlacZ/+TA muscle sections stained for X-gal (blue) and AP (red); Pecam is stained brown in (o). (ad) Scale bar, 100 μm. (eo) Scale bar, 20 μm.