Figure 6 : Brain tight junctions and cytokine expression.

From: Low-dose penicillin in early life induces long-term changes in murine gut microbiota, brain cytokines and behavior

Figure 6

(ad) mRNA expression of tight junctions measured in the hippocampus and the frontal cortex of male and female mice. (eg) Representative western blot of tight junctions proteins claudin-5 and occludin performed in hippocampus of male mice and quantification by densitometry normalized to the loading control β-actin (in total: n=5 CT, 8 AB and 4 AB/JB1 mice). Full blots are shown in Supplementary Fig. 22. (hk) mRNA expression of cytokines and chemokine in hippocampus and frontal cortex of male and female mice. Results are means±s.e.m. (Hippocampus n=24 males (9 CT, 11 AB, 4 AB/JB1) and 39 females (15 CT, 11 AB, 13 AB/JB1); frontal cortex n=28 males (11 CT, 12 AB, 5 AB/JB1) and 42 females (17 CT, 13 AB, 12 AB/JB1)) (one-way ANOVA) *P<0.05, **P<0.01, ***P<0.001 versus CT. #P<0.05 versus AB and $P<0.10 versus AB. AB, antibiotic; AB/JB1, antibiotic and L. rhamnosus JB-1; CT, control.