Figure 1: VLP-hCEA eradicates intestinal tumours and substantially extends the lifespan of hCEA-Tg/ApcMin/+ mice. | Nature Communications

Figure 1: VLP-hCEA eradicates intestinal tumours and substantially extends the lifespan of hCEA-Tg/ApcMin/+ mice.

From: Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of ApcMin/+ mice

Figure 1

(a) VLP-hCEA immunization scheme. (b) Methylene blue staining of the small intestines from hCEA-Tg/ApcMin/+ mice that were orally immunized with either PBS or VLP-hCEA pseudovirus. Ruler scale, 1 mm. (c) Representative haematoxylin and eosin (H&E) staining of small intestines after PBS or VLP-hCEA oral immunization in hCEA-Tg/ApcMin/+ mice. Scale bars, 200 μm. (d) The number of intestinal polyps of different sizes from hCEA-Tg/ApcMin/+ mice (n=7 for both groups, including 4 male and 3 female mice per group) that were immunized as in (b). Results are shown as mean±s.d. Student’s t-test was performed to determine the statistical significance. Data are representative of three independent experiments. (e) Representative pictures of spleens from PBS or VLP-hCEA orally immunized hCEA-Tg/ApcMin/+ mice. (f) The ratios of mouse spleen to whole body weight of hCEA-Tg/ApcMin/+ mice (n=7 for both groups, including 4 male and 3 female mice per group) that were immunized as described in (b). Results are shown as mean±s.d. Student’s t-test was performed to determine the statistical significance. Data are representative of three independent experiments. (g) Survival of hCEA-Tg/ApcMin/+ mice that were orally immunized with PBS (n=18, including 11 male and 7 female mice) or VLP-hCEA (n=24, including 13 male and 11 female mice). Log-rank test was performed to determine the statistical significance. Data are representative of two independent experiments. (h) Body weights of 20-week-old hCEA-Tg/ApcMin/+ mice that were orally immunized with either PBS (n=8, including 4 male and 4 female mice) or VLP-hCEA (n=10, including 6 male and 4 female mice). Results are shown as mean±s.d. Student’s t-test was performed to determine the statistical significance. Data are representative of three independent experiments. ***P<0.001.

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