Table 2 Characterization of natural killer cell populations of wild mice and their comparison to laboratory mice.

From: The comparative immunology of wild and laboratory mice, Mus musculus domesticus

Parameter Laboratory Wild  
Female Male Female versus Male Female Male Female versus Male Lab. versus Wild
1NKp46+ (%) 1.9 (0.5–4.7; 36) 3.1 (0.2–7.4; 24) U=636** 1.5 (0.7–3.5; 60) 1.5 (0.2–5.4; 77) U=2419 H=28.3***, LF>WF*, LM>WF***, LM>WM***
2NKp46+ stage 1 (%) 9.2 (3.2–38.0; 36) 6.3 (2.5–27.2; 22) U=147*** 10.4 (4.6–39.1; 53) 12.3 (3.6–45.4; 64) U=1575 H=22.4***, LM<WF***, LM<WM***
2NKp46+ stage 2 (%) 17.9 (8.3–88.4; 36) 18.3 (10.4–93.3; 22) U=508 29.6 (8.0–71.0; 53) 36.2 (15.2–71.0; 64) U=2031 U=1362***
2NKp46+ stage 3 (%) 29.7 (0.06–55.8; 36) 34.7 (0.06–50.8; 22) U=431 21.7 (7.3–50.0; 53) 20.3 (5.4–45.5; 64) U=1747 U=4656***
2NKp46+ stage 4 (%) 38.4 (0.08–58.7; 36) 34.2 (0.06–52.9; 21) U=298 32.4 (4.3–58.9; 53) 26.6 (5.1–54.1; 64) U=1423 U=4431**
NK stages 1&2 : 3&4 ratio 0.40 (0.13–665; 36) 0.34 (0.16–1666; 22) U=363 0.80 (0.26–7.33; 53) 0.99 (0.25–6.33; 64) U=1954 U=1542***
NKp46+ stage 1 CD69 (MFI) 94 (35–335; 36) 92 (51–207; 21) U=352 132 (38–282; 53) 150 (81–525; 64) U=2166** U=1119***
NKp46+ stage 2 CD69 (MFI) 232 (71–533; 36) 126 (76–254; 21) U=214** 203 (50–681; 53) 202 (110–991; 64) U=1884 H=14.8**, LM<WF*, LM<WM***
NKp46+ stage 3 CD69 (MFI) 119 (57–515; 36) 85 (69–1104; 21) U=316 221 (50–916; 53) 261 (120–1142; 64) U=2105* H=54.2***, LF<WF***, LF<WM***, LM<WF***, LM<WM***
NKp46+ stage 4 CD69 (MFI) 85 (30–207; 36) 65 (45–120; 20) U=234* 200 (67–750; 53) 212 (89–984; 64) U=2082* H=89.0***, LF<WF***, LF<WM***, LM<WF***, LM<WM***
NKp46+ stage 1 KLRG1 (MFI) 91 (67–324; 18) 83 (47–200; 19) U=135 92 (19–261; 43) 106 (17–341; 46) U=1113 U=1574
NKp46+ stage 2 KLRG1 (MFI) 30 (1–44; 15) 20 (7–72; 17) U=136 9 (1–38; 24) 9 (1–65; 27) U=350 U=1256***
NKp46+ stage 3 KLRG1 (MFI) 133 (79–351; 18) 93 (57–967; 19) U=110 57 (5–280; 41) 71 (10–1585; 42) U=105 U=2334***
NKp46+ stage 4 KLRG1 (MFI) 1358 (727–1978; 18) 1121 (602–2730; 19) U=114 926 (55–2872; 43) 1143 (99–3895; 49) U=1279 U=2158**
2NKp46+ Ly49D+ (%) 48.5 (0.6–81.0; 31) 54.6 (47.2–60.5; 18) U=421** 71.1 (41.8–80.4; 56) 71.3 (52.8–86.7; 69) U=2039 H=80.9***, LF<WF***, LF<WM***, LM<WF***, LM<WM***
2NKp46+ Ly49G2+ (%) 39.6 (0.2–96.3; 31) 47.6 (44.2–53.1; 18) U=424** 30.3 (12.9–59.4; 56) 29.4 (6.7–66.3; 69) U=1810 H=34.2***, LF>WM*, LM>WF***, LM>WM***
2NKp46+ Ly49H+ (%) 46.0 (0.1–88.9; 31) 58.3 (44.9–64.8; 18) U=475*** 0.13 (0.0–16.7; 56) 0.12 (0.0–46.0; 69) U=1774 H=97.8, P<0.001***, LF>WF***, LF>WM***, LM>WF**, LM>WM***
2NKp46+ Ly49D+G2- (%) 23.55 (0.55–49.50; 31) 24.41 (18.45–27.99; 18) U=343 44.57 (15.62–63.68; 56) 46.00 (20.95–68.29; 69) U=2022 U=431***
2NKp46+ Ly49D+G2+ (%) 23.30 (0.07–96.30; 31) 31.35 (26.70–33.82; 18) U=424** 26.76 (11.60–53.10; 56) 25.40 (5.79–61.72; 69) U=1793 H=11.9**, LM>WM*
2NKp46+ Ly49D-G2+ (%) 15.34 (0.00–38.65; 31) 16.70 (13.27–22.35; 18 U=372 4.18 (0.74–6.80; 56) 3.88 (0.31–7.62; 69) U=1722 U=5765***
2NKp46+ Ly49D-G2- (%) 35.09 (0.02–75.40; 31) 26.76 (23.97–32.80; 18) U=111*** 23.24 (14.68–56.24; 56) 24.03 (11.03–41.76; 69) U=1981 H=37.9***, LF>WF***, LF>WM***
  1. The table shows the natural killer cell populations for wild and laboratory mice, shown as medians (range; n). Statistical results show comparisons between sex within animal source (that is, laboratory or wild mice), and between animal source. Where there exists a significant difference between sex, mice were grouped by sex and animal source as: laboratory female, laboratory male, wild female and wild male (LF, LM, WF, WM, respectively), with Kruskal–Wallis H-tests (and post hoc, Dunn–Bonferonni, pairwise comparisons) conducted with significant differences reported. All non-normal data were analysed by Mann–Whitney U-tests, unless stated otherwise. Asterisks denote significant differences as *P<0.05, **P<0.01, ***P<0.001. Superscripts define cell populations as a percentage of: 1spleen cells; 2NK cells.