The concentrations of nine cytokines (IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, IL-13, MIP-2α) produced by splenic lymphocytes stimulated with anti-CD3/anti-CD28, CpG, LPS or PG compared with the RPMI control in wild (shaded) and laboratory (unshaded) mice, shown on a log10 scale. The box centres are medians, and box limits the 25th and 75th percentiles, the whiskers 1.5 times the interquartile range, and outliers are represented by dots. The dotted horizontal lines show the median lower limit of quantification defined from standard curves across all analysed plates for each cytokine. Asterisks denote significant differences as *P<0.05, **P<0.01, ***P<0.001 (Mann–Whitney U test; Supplementary Table 5). Sample sizes are shown in Supplementary Data 1 and Supplementary Table 5.