Figure 5: Myeloid cells. | Nature Communications

Figure 5: Myeloid cells.

From: The comparative immunology of wild and laboratory mice, Mus musculus domesticus

Figure 5

(a) The flow cytometry gating strategy to identify CD11b+ CD11c myeloid cells and the proportion of myeloid cells among splenic leukocytes in wild (shaded) and laboratory (unshaded) mice, (b) gating myeloid cells on F4/80 and Ly6G expression to define M1 (tissue resident macrophages), M2 (monocytes), M3 (hypergranulocytic myeloid cells, HGMC) and M4 (polymorphonuclear leukocytes, PMN) subsets, (c) Ly6G expression confirming the presence of three cell populations in laboratory mice and four populations in wild mice, (d) side scatter characteristics of the M1–M4 populations in wild (shaded, n≥115) and laboratory (unshaded n≥57) mice; note that too few cells were present in the M3 gate in laboratory mice to accurately determine a side scatter statistic, (e) scatter characteristics of M3 (left) and M4 (right) cells, revealing a low forward scatter neutrophil population (M5) and a high forward scatter myeloid derived suppressor cell population (M6) among the M4 cells, (f) proportions of M1, M2, M3 and M4 subpopulations among the myeloid cell population in wild (shaded) and laboratory (unshaded) mice, and (g) gating of CD11c+ dendritic cells and their proportions among splenocytes in wild and laboratory mice. For the box plots, box centres are medians, and box limits the 25th and 75th percentiles, the whiskers 1.5 times the interquartile range and outliers are represented by dots. Asterisks denote significant differences as *P<0.05, ***P<0.001 (Mann–Whitney U test; Supplementary Table 2), and § denotes that there are additional sex effects detailed in Supplementary Table 2. Sample sizes are shown in Supplementary Table 2 and Supplementary Data 1.

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