The flow cytometry gating strategy and proportions of subsets of CD3+ T cells in wild (shaded) and laboratory (unshaded) mice for (a) CD4+ cells, (b) CD4+ Treg cells, (c) CD8+ cells and their maturation state and (d) terminally differentiated CD8+ cells. CD4+ and CD8+ effector/effector memory cells are defined as CD62L− CD44hi and central memory cells are CD62L+ CD44hi. The box centres are medians, and box limits the 25th and 75th percentiles, the whiskers 1.5 times the interquartile range and outliers are represented by dots. Asterisks denote significant differences as *P<0.05, **P<0.01, ***P<0.001 (Mann–Whitney U test; Supplementary Table 2), and § denotes that there are additional sex effects detailed in Supplementary Table 2. The gating strategy for CD3+ lymphocytes is shown in Supplementary Fig. 1. Sample sizes are shown in Supplementary Table 2 and Supplementary Data 1.