(a) Schematic cross-section of the cochlea showing cells of the sensory epithelium (organ of Corti) including inner pillar cells (IPCs), outer pillar cells (OPCs), Deiters’ cells (DCs), Hensen cell (HC), outer hair cell (OHC), inner hair cell (IHC), Claudius cells (CCs) and major non-cellular elements (basilar membrane (BM), tectorial membrane (TM) and reticular laminar (RL); modified with permission from Fig. 1 (ref. 49). (b) Confocal micrograph of 10 μm cryosection taken from middle turn of cochlea in c to identify details of cells and noncellular structures in the organ of Corti. Modified from c. Rows of confocal micrographs of 10 μm cryosections of the apical, middle and basal turns of the organ of Corti and stria vascularis. The rows are organized in columns of pairs of micrographs at each location from CD-1Cx30WT/WT, CD-1Cx30A88V/A88V and CBA/J mice. The left of each pair of micrographs shows the unstained section. In the right of each pair, the Cx30 (red) expression is revealed with a selective antibody and nuclei are counterstained with DAPI (blue). OHCs, DCs and spiral lamina cells are intact in all cochlea turns of CBA/J and CD-1Cx30A88V/A88V mice but not in the basal turn of CD-1Cx30WT/WT mice. Cx30 appears to be localized in the membranes in basal cells of the stria vascularis, DCs, IPCs, OPCs and spiral lamina cells of the intact OC, that is, in all turns of the cochleae of CBA/J and CD-1Cx30A88V/A88V mice. Scale Bar, 35 μm (b) and 80 μm for all micrographs in c, see blue and grey squares in a for location of histology shown in rows 1–3 (organ of Corti) and 4 (stria vascularis), respectively. All mice were 3 months old.