Figure 5: Negative stain EM of DegP and DegP-loaded DNA hosts. | Nature Communications

Figure 5: Negative stain EM of DegP and DegP-loaded DNA hosts.

From: Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions

Figure 5

(a) Representative digital micrograph area of negatively stained DegP12 and DegP24-loaded DNA cages. Scale bar, 100 nm. Representative class averages, each containing 50–100 particles, for DegP6 (b), DegP12 (c) and DegP24 (d). Scale bar, 10 nm. The simulated 3D models from the respective crystal structures downfiltered to a resolution of 15 Å are also indicated. PDB-ID codes for DegP6 (b), DegP12 (c) and DegP24 (d) are, respectively, 1ky9, 2zle and 3cS0. Two-dimensional analysis of empty (e), DegP6 (f), DegP12 (g) and DegP24 (h) loaded DNA origami hosts bearing 18 convergent PAs in their cavity (6p120-18cA1). Representative class averages, each containing 25–100 particles, and raw particle images of the corresponding classes are reported, together with a model of each construct, both in top and front view. Scale bar, 10 nm.

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