(a) Logic circuit diagram, seesaw circuit diagram and experimental data of a two-layer logic circuit. (b) Measuring the effective concentrations of the gate species. Three independent circuits were used to measure the effective concentrations of two gates fully triggered by x1 and x2, respectively, comparing with the effective concentration of x3 (using signal strand w18,53). (c) Experimental data of the two-layer logic circuit using adjusted nominal gate concentration. 1 × =100 nM.