Figure 6: HHH traps at intermediate concentrations of FW. | Nature Communications

Figure 6: HHH traps at intermediate concentrations of FW.

From: Mechanism of partial agonism in AMPA-type glutamate receptors

Figure 6

(a) Trapping at different concentrations of FW. Three records from different patches showing trapping after the application of Zn2+ (10 μM) for 100 nM (dark blue), 3 μM (orange) and 100 μM FW (light blue). The inset shows the current after trapping displaying a robust reduction occurring at 3 μM. The fits (dots) are double exponentials fitted to the recovery after trapping the arrows and symbols indicate measurements plotted in c. (b) Currents from WT GluA2 were not modified at any concentration of FW. (c) Dose–response curves for FW in Zn2+ and 100 μM CTZ for WT GluA2 (EC50=1.7±0.2 μM) (closed blue circles) and the mutant HHH (closed yellow circles) (EC50=2.7±0.3 μM). The active fraction for WT GluA2 is flat (blue open dots). The active fraction for the HHH mutant (open yellow circles) is fit by a log-normal function with a maximum inhibition at 3 μM FW. All error bars are s.e.m. (n=5).

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