Figure 6 : The nutrient sensor AAK-2/AMPK regulates FLP-7 release from ASI neurons.

From: A tachykinin-like neuroendocrine signalling axis couples central serotonin action and nutrient sensing with peripheral lipid metabolism

Figure 6

(a) Representative images of wild-type, aak-2, ctrc-1 and aak-2;crtc-1 animals, with the indicated rescuing transgenes in the FLP-7mCherry and CLM::GFP secretion line. Left panels, GFP expression in coelomocytes; centre panels, secreted FLP-7mCherry uptake in coelomocytes; right panels (merge). Scale bar, 10 μm. (b) For vehicle- and 5-HT-treated animals bearing integrated FLP-7mCherry and CLM::GFP transgenes, the intensity of FLP-7mCherry fluorescence within a single coelomocyte was quantified and normalized to the area of CLM::GFP expression. Genotypes are indicated in the figure. Data are expressed as a percentage of the normalized FLP-7mCherry fluorescence intensity of vehicle-treated wild-type animals±s.e.m. (n=10–25 animals). *P<0.05 and **P<0.01, ***P<0.001 by one-way ANOVA. (c) mCherry fluorescence intensity values are plotted against GFP fluorescence intensity values for each animal across representative experimental conditions, n=58. (d) Fat content was quantified in wild-type animals and aak-2, crtc-1, aak-2;crtc-1 mutants and aak-2;crtc-1 mutants bearing a aak-2 transgene the ASI-specific gpa-4 promoter, as indicated. Data are expressed as a proportion of fat retained upon 5-HT treatment±s.e.m. (n=18–20). **P<0.01 and ***P<0.001 by two-way ANOVA.