Figure 4 : FLP-7 secretion in response to 5-HT and OA receptors.

From: A tachykinin-like neuroendocrine signalling axis couples central serotonin action and nutrient sensing with peripheral lipid metabolism

Figure 4

(ac) Representative images of vehicle-, 5-HT- and Octopamine (OA)-treated wild-type, mod-1, ser-6, and mod-1;ser-6 animals bearing integrated FLP-7mCherry and CLM::GFP transgenes, respectively. Left panels, GFP expression in coelomocytes; centre panels, secreted FLP-7mCherry uptake in coelomocytes; right panels (merge). Scale bar, 10 μm. (d) The intensity of FLP-7mCherry fluorescence was quantified and normalized to the area of the CLM::GFP. Genotypes are indicated in the figure. Data are expressed as a percentage of the normalized FLP-7mCherry fluorescence intensity of vehicle-treated wild-type animals±s.e.m. (lower panels; n=11–27). ***P<0.001 and NS, not significant by two-way ANOVA for within-group analyses. (a) P<0.01 by two-way ANOVA compared to 5-HT-treated wild-type animals. (b) P<0.05 by two-way ANOVA compared to OA-treated wild-type animals.