Figure 2 : The tachykinin-related neuropeptide FLP-7 functions in the ASI neurons to regulate 5-HT-induced fat loss.

From: A tachykinin-like neuroendocrine signalling axis couples central serotonin action and nutrient sensing with peripheral lipid metabolism

Figure 2

(a) Fluorescent image of a transgenic animal bearing a polycistronic flp-7::mCherry transgene under the control of the endogenous flp-7 promoter. White arrowheads indicate expression in ASI neurons, the open arrowhead indicates expression in ALA and the grey arrowhead indicates expression in AVG. A, anterior; P, posterior; V, ventral; D, dorsal. Scale bar, 20 μm. (b) Fat content of vehicle- and 5-HT-treated flp-7 mutants bearing a flp-7 transgene using the indicated promoters was measured. Relative to non-transgenic controls, transgenic flp-7 animals bearing the flp-7 transgene under the control of the endogenous flp-7 promoter and the heterologous ASI promoters daf-7 and str-3 restore 5-HT-induced fat loss indistinguishably from wild-type animals. Data are expressed as a proportion of fat retained upon 5-HT treatment±s.e.m. (lower panels; n=8–20). **P<0.01 and ***P<0.001 by two-way ANOVA. (c) flp-7 was inactivated in wild-type animals using RNAi-mediated antisense. Relative to non-transgenic controls, transgenic wild-type animals bearing flp-7 sense-antisense transgenes under the control of the ASI-specific daf-7 promoter suppress 5-HT-induced fat loss, as seen in flp-7 mutants. Data are expressed as a proportion of fat retained upon 5-HT treatment±s.e.m. (lower panels; n=10–24). ***P<0.001 by two-way ANOVA.