Figure 1 : Neuropeptide signalling is required for 5-HT-mediated fat loss.

From: A tachykinin-like neuroendocrine signalling axis couples central serotonin action and nutrient sensing with peripheral lipid metabolism

Figure 1

(a,c) Vehicle- and 5-HT-treated animals were fixed and stained with oil Red O. Genotypes are indicated in the figure. Fat content for each genotype was quantified and expressed as a percentage of vehicle-treated wild-type animals±s.e.m. (n=10–14). ***P<0.001 and NS, not significant by two-way ANOVA. (b) Representative images of vehicle- or 5-HT-treatment, fixed and stained with oil Red O in the indicated genotypes. Animals are oriented facing upwards with the pharynx at the anterior end. Lipid droplets that store body fat are restricted to the intestinal cells. (d) Wild-type animals (black bars) overexpressing the flp-7 transgene (OX; grey bars) grown on vector or atgl-1 RNAi containing bacteria were fixed and stained with oil Red O. Data are expressed as a percentage of body fat in wild-type (non-transgenic) animals±s.e.m. (lower panels; n=11–21). *P<0.05 and **P<0.01 by two-way ANOVA. (e) Representative images of vehicle- and 5-HT-treated wild-type animals and flp-7 mutants bearing an integrated atgl-1::GFP transgene. Scale bar, 50 μm. (f) The fluorescence intensity of atgl-1 expression in vehicle- and 5-HT-treated wild-type animals and flp-7 mutants was quantified and is expressed as a percentage of vehicle-treated wild-type animals±s.e.m. (17–27). **P<0.01, ***P<0.001 and NS, not significant by two-way ANOVA. (g) Feeding rate is expressed as a percentage of vehicle-treated wild-type animals±s.e.m. (n=27). Genotypes are indicated in the figure **P<0.01 and NS, not significant by two-way ANOVA. (h) Egg-laying rates were measured in vehicle- and 5-HT-treated wild-type animals and flp-7 mutants. For each genotype and condition, the average number of eggs laid was counted as described in the methods. Data are expressed as an average±s.e.m. (n=10). **P<0.01 and ***P<0.001, by two-way ANOVA. (i) The number of body bends over a 20-s interval was counted in the presence and absence of the bacterial food so`urce. Data are expressed as an average±s.e.m. (n=10–16). ***P<0.001 by two-way ANOVA.