(a–e) Azole formation in the FIT–GodD/E system was investigated with GodA*1–9 (a), GodA*1–6 (b), GodA*1–3 (c), GodA*1–3-T2C (d) and GodA*1–3-V3Y (e). (f,g) The order of Dha formation was investigated with GodA*-S4A (f) and GodA*-S14A (g) using the FIT–GodD/E/F/LazF system. (h–j) Sequence requirement of the LP region in the FIT–GodD/E/F/LazF system was investigated with GodA*LP(−20)–(−1) (h), GodA*LP(−15)–(−1) (i) and GodA*LP(−10)–(−1) (j). (k,l) noncanonical azole formation on the designer GodA* analogue in the FIT–GodD/E system was investigated with GodA*-10SA11 (k) and GodA*-tandem (l). Calculated and observed m/z values in MALDI–TOF–MS analyses are summarized in Supplementary Data.