Figure 4: HOPX partially suppresses EMT in NPC cells. | Nature Communications

Figure 4: HOPX partially suppresses EMT in NPC cells.

From: HOPX hypermethylation promotes metastasis via activating SNAIL transcription in nasopharyngeal carcinoma

Figure 4

(a) Phase contrast images (× 200) of N2-Tert and NP69 cells expressing control NC or HOPX-siRNAs (si-1 and si-2). Scale bar, 100 μm. (b,c) Immunofluorescence images (× 600) for HOPX, ECADHERIN, α-CATENIN and VIMENTIN expression in N2-Tert and NP69 cells transiently expressing control NC or HOPX-siRNAs (si-1 and si-2) (b), and CNE2 and SUNE1 cells stably overexpressed the vector or HOPX (c). Scale bar, 100 μm. (d,e) Western blotting analysis of ECADHERIN, α-CADHERIN, VIMENTIN, FIBRONECTIN, HOPX and GAPDH expression in N2-Tert and NP69 cells transfected with the control NC or HOPX-siRNAs (d), and CNE2 and SUNE1 cells stably overexpressed the vector or HOPX (e). These data are representative of three independent experiments.

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