Figure 3: Effects of EtOH on membrane electrical properties of Agrp neurons. | Nature Communications

Figure 3: Effects of EtOH on membrane electrical properties of Agrp neurons.

From: Agrp neuron activity is required for alcohol-induced overeating

Figure 3

(a) Effect of 50 mM EtOH on spike rate in cell-attached recordings. Representative example of six cells. Firing before EtOH=1.85±0.35 Hz, after=3.72±0.52 Hz, paired t-test: t=4.613, d.f.=5, P=0.0058. (b) Effect of 50 mM EtOH on membrane potential, under synaptic blockade with of 10 μM CNQX and 50 μM PTX. Representative example of eight cells, spikes are truncated at +20 mV. Firing before EtOH 1.07±0.22 Hz, after=7.18±1.58 Hz, paired t-test: t=3.757, d.f.=7, P=0.0071. (c) Effect of 50 mM of EtOH on membrane potential in whole-cell recordings, under synaptic blockade with 1 μM TTX. Top, membrane potential response; Bottom, responses to hyperpolarizing current injections (resting potentials artificially aligned to dotted line to facilitate comparison). Representative example of eight cells. Mean depolarization was 9.38±0.82 mV, one-sample t-test: t=11.4, d.f.=7, P<0.0001. (d) Effect of 50 mM EtOH on whole-cell current–voltage relation. Left, membrane voltage ramps (representative example of 5 cells). Right, net EtOH-induced current (means±s.e.m., n=5 cells). (e) Effect of 50 mM EtOH on membrane potential (left, spikes are truncated at 0 mV), and net EtOH-induced current (right), in the presence of 10 mM intracellular BAPTA. Representative examples of nine cells. Firing before EtOH=0.96±0.21 Hz, after=0.92±0.20 Hz, paired t-test: t=1.110, d.f.=8, P=0.2994. (f) Effect of 50 mM EtOH on membrane potential (left, spikes are truncated at 0 mV), and net EtOH-induced current (right), in the presence of 70 μM KB-R7943. Representative example of n=8 cells. Firing before EtOH=0.64±0.19 Hz, after=0.62±0.17 Hz, paired t-test: t=0.4760, d.f.=7, P=0.6485.

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