(a) Effect of EtOH on daily food intake (one 2 g kg−1 i.p. EtOH injection per day for 3 days; see Methods). Values are averages per mouse per day. Pre=3 days before EtOH, post=3 days after ethanol (mice i.p. injected with saline on non-EtOH days). One-way repeated measures analysis of variance: males, treatment F(2, 10)=21.82, P=0.0002, n=6 mice; females, treatment F(2, 6)=7.628, P=0.0225, n=4 mice. Numbers between bars are P values from Tuckey’s multiple comparison corrections. Bars and error brackets are means±s.e.m. (b) EtOH-induced food intake in males versus females from experiment in A. Two-tailed unpaired t-test, t=0.7286, d.f.=8, P value indicated between bars. Numbers above bars are P values from one-sample t-tests: males, t=3.3672, d.f.=5; females, t=3.5242, d.f.=3. Bars and error brackets are means±s.e.m. (c) Food intake induced by EtOH injection (arrowed, 2 g kg−1), shown at hourly time points (n=4 male mice, ‘food intake increase’ was calculated by subtracting food intake after saline injection from food intake after EtOH injection, for each mouse and time point). Values are means±s.e.m.