The subset of T cell traits shown in Fig. 2a are expanded. (a,b) T-cell traits for non-CD4 T cells (a), including CD8 T, γδ T and NKT cells (defined as CD1d-multimer binding), and for CD4 T cells (b). Traits were arranged according to the subsets from which they were defined: ‘Differentiation’—subsets defined by the markers CD27, CD28, CD31, CD45RA, CD57, CD95, CD127 and CD244 (Staining panel 1; see Supplementary Fig. 2 in Roederer et al.8). ‘Activation’—subsets defined by the markers CD25, CD38, HLA-DR and CD279/PD-1 (Staining panel 2). ‘Polarization’—subsets defined by the markers CCR4, CCR6, CCR10, CXCR3 and CXCR5 (Staining panel 3). ‘Treg’—subsets defined by the markers CD25, CD127, CD39, CD45RO and CD73 (Staining panel 2). (c) Traits for only Treg cells (expressing CD39 and/or CD73). Of the 20 traits showing shared environmental influence, 18 arise from subsets co-expressing CD25 and CD73 with variable expression of other markers.